KAUSHAL SAHU

biochemistry biomolecules chemistry protein function classification plant tissue culture animal cell culture molecular marker science bioinformatics technology carbohydrates polysaccharides types carbohydratees types of protein ddbj primary structure of protein protein motiff ncbi embl amino acid translation protein synthesis pka types of amino acid nanotechnology essential amino acid nanobiotechnology sds page eelectrophoresis chromatography recombination repair structural classification of protein dna sequencing application of bioinformatics application saccharides genetic mapping dna repair transcription functional proteomics p53 gene prb gene lipoprotein biotechnology ribosome global analysis of protein analysis of protein snp cell culture buffer hydrogen bond stem cell culture ph weak bond biomolécule gene bank blast glycoprotein stem cell culture and application application of stem cell culture genetic map insect resistant plant genomic mapping plant secondary metabolites physical map bt gene transgenic animal transgenic mouse transgenic mice genetic manipulated animal enzyme kinetics macromolecules complex protein sequencing chromatin measurement tools of nanotechnology tissue engineering fasta cell cloning swiss prot secondary biological database primary biological database biological database application of nanobiotechnology single nucleotide polymorphism mass spectrometry immunoprecipitation yeast two hybrid system protein protein interaction proteomics functional genomics tool functional genomics physical mapping ion exchange transcription factor secondary metabolites bond interaction disaccharides affinity dna repair and recombination enzyme involved in dna replication polyacrylamide agarose gel electrophoresis types of dna repair protein folding protein ligand interaction hplc splicing enzyme rna splicing mrna splicing recombinase electrophoresis lipid significance tumor suppresor gene acid dissociation constatnt vaccine southern blotting biotechnology information system in india btis respiration drug discovery bioinformatics in drug discovery bioinformatic tools hydrophobic interaction pdb hydrophoic bioinformatics tools application of nanotechnology polynucleotide michelis mentan equation structure and function of lipoprotein protein separation and purification biology rflp rapd enzyme immobilization fluorescence fluorescence spectroscopy stem tem sem database primary and secondary database microarray technology dna chip aflp biochip microarray marker application of cheminformatics general concept of cheminformatics cheminformatics btis net in chhattisgarh excellence center apex center blotting secondary structure of protein ti plasmid ramchandran map disease resistant plant plasmid vector plasmid binary vector virus resistant plant sterilization media shoot induction organogenesis micropropagation embryogenesis glycogen somatic embryogenesis column chromatography structure analysis of protein liquid nitrogen germplasm storage cryopreservation cybrids protoplast fusion protoplast isolation plant biotechnology btis net ph scale pharmaceutical medical field ionic bond ribozyme catalytic rna protein chip protein microarray protein array titration curve types of proteomics monosaccharides myoglobin automated dna sequencing sanger method dna sequencer deoxyribose sugar storage of lipid tpes of storage lipid fat oils waxes plant genomics t dna haploid plant production follicle stimulating hormone (fsh) base excision repair nucleotide excision repair hormones that are glycoprotein thyroid stimulating extra cellular matrix mismatch repair somatic cell genetics totipotency michaelis-menten equation derivation cell genetics techniques of cell cloning michaelis-menten curve application of cell cloning techniques types of recombinase cre recombinase hin recombinase application of tissue engineering tre recombinase rec a recombinase alternative splicing artificial skin dna replication recombination viral glycoprotein enzymes that are glycoprotein aberrant growth control immortalization cell transformation cell lines human chorionic gonadotropin (hcg) holliday model messelson and radding model double strand break model gene conversion synchronous culture cell synchronization non-homologous recombinati site specific recombination transpositional recombination. erythropoietin hormone luteinizing hormone (lh) direct repair system artificial cartilage ligand interaction eukaryotic translation machinery artificial organ translation cycle transgenic fish prokaryotic translation protein modification conformational propeties of polysaccharide co translation modification post translation modification translation machinery receptor mediated endocytosis translation in eukaryotes macromolecules prokaryotic translation machinery organization of macromolecules complex inuline cellulose vector chitin compositional analysis of polysaccharides starch qualitative quantitative sugar classification and reaction sugar resistant plant size exclusion viruse production of disease resistant plant catalytic ab structure of protein characterization of protein gas chromatography mobile phase stationary phase column oven detector helicase peptide bond rotation enzyme involve in dna replication protein involve in dna replication super secondary structure structural motiff hairpin meander alpha corner functional protein dna hybridization nucleic acid hybridization gas rna enzyme structureof protein ramchandran plot map genetic engineering catalytic antibody heteropolysaccharides homopolysaccharides deep freeze culture stage one bt cotton initiation of culture stage one aseptic technique sterilizing instrument abzyame maintanace of aseptic condition culture media michaelis-menten equation enzyme effect micronutrients enzyme effect of organic solvent enzyme catalysis protein stability stability of protein shoot multiplication cytokinin hormone growth regulator denaturation of protein tissue culture media antibody enzyme nutrients preparation of culture media thawing cryoprotectent freezing method embryo culture hardening analysis of proetin biodegradable plastic plastic auxin renewable plastic advantage of biodegradable plastic anther culture culture stage 3 cross linking covalent binding micropropagation stage 3 encapsulation rooting entrapment hybrid selection browning of medium mechnical method adsorption reversible inhibition explant amp production utiization tca transcription termination modificatioon of mrna eukaryotic transcription transcription in eukaryotes rna polymerase ploy a tail tyes of bond h bond h hydrogen biomollecule tailing of mrna g capping of mrna acid constant henderson – hasselbalch equation homologous recombination henderson – hasselbalch equation weak interaction holiday junction model vanderwals bond boond physics thermodynamics law of thermodynamics types of bond strong bond tumor suppressor gene synthesis of membraane protein membrane protein mendels law transcription in prokaryotes charecterstics of molecular marker prokaryotic transcription enzyme kinetics and thermodynamic analysis endocytosis ssr edocytosis protein shorting and targeting plant protein targeting alkaloids metabolic pathway propaoids restriction enzyme protein shorting autoradiography eddible vaccine immunology potato food technology translation in prokaryotes php energy activation energy biological system cre lox recombination actin myocin nucleotides nucleosides phosphoric acid pgment light absorbing pigment chloroplast porphyrin structure of porphyrin chemistry of porphyrin biomedical importance dna replication in prokaryotes prokaryotic dna replication dna replication in eukaryotes eukaryotic dna replication ligase primase phospholipd glycolipid cholestrol membrane lipid bilayers agarose topoisomerase use ssbp tae buffer organ formation in plant atp vander walls bond covelant bond holiday junction mitochondrial enzyme hdlv group 1 intron group two intron hamerhead protein interaction liganad iso enzyme clinical significance alkaline phosphatase lactate dehydrogenase glycosylation mode of action ss bond disulphide bond types of dna damage elerochemical properties ionization dna damage and repair peptide peptide synthesis titration curve of amino acid dna damage cre lox flp frt cre lox flp frt recombination isoprenoid animal cell culture media constituents of culture media history of bioinformatics scope in bioinformatics introduction to ncbi 3d cell culture 2d cell culture application of animal cell culture supramolecules assembly microarray database tertiary structure of protein nanoscale phamacogenomics edman degradation method tertiary biological database blotting techniques northern blotting western blotting centrifugation centrifugal force ultra centrifugation centrifugation techniques chemoheterotrophs photosynthetic microorganism photosynthesis in micro organism photosynthetic bacteria hardy weinberg law hardy weinberg genetic equilibrium population genetics densitometry beers lambert law dna microarray bicarbonate and buffering co2 advanatage of naanobiotechnology drawback of nanobiotechnology balanced salt solutions constituent of animal tissue culture media contact inhibition fasat tumorigenicity transformed cell chromosomal aberation tumorogenecity bioinformatic bioinformatics in biotechnology genetic instability software for bioinformatics bioinformatics relevance with biotechnology bioinfromatics bioinformatics biotechnology molecular medicine microbial genomics animal genomics cell culture based vaccine cell culture and maintenance cell lines used in screening techniques cloning in animal cell culture animal cell growth cultured cell biology and characterization of the cell culture cell lines application animal cell lines bright field microscopy ria radio immuno assay hepatitis b virus spectrometry ir spectrometry raman spectrometry uv visible spectrometry microscope types of microscope pcr types of pcr real time pcr gradient pcr x ray x ray diffraction diffraction immobilization of enzyme encapsualtion antrapment n terminal sequencing c terminal sequencing rna sequencind nucleic acid sequencing rna protein gene sequencing strategies gene sequencing types of polynucleotides conformation properties of polysaccharides conformational properties of polynucleotide conformational propeties of polynucleotide lineweaver burk plot midea constituents electron microscope scanning electron microscope c terminal analysis n terminal analysis transmission electron microscope nucleic acid sequencind scanning transmission electron microscope paper electrophoresis flowcytometry cytophotometry cytometer rna dna high performance liquid chromatography hptlc ion exchange chromatography affinity chromatography mathanogenes bio gas bio gas system mathanobacter elisa elisa test enzyme linked immuno sorbent assay direct elisa indirect elisa competative elisa nmr nuclear magnetic resonance principle of nmr microscopy shikimate pathway metabolic engineering phenylpropanoid pathway map based cloning of genome cloning synthesis of secondaary metabolites sscp single strand conformational polymorphism amplified fragment length polymorphism chloroplast genome plant genome map genetic marker mapping stem cell therapy genome mapping gene medicine gene transfer genomic medicine functional genomics tools functional genomics websites genomic tools reverse genetics forward genetics comparative genomics in eukaryotes caomparative genomics in organelles comparative genomics types of genomics scale up of animal cell culture scale up primary culture and cell line mexam gilberts method pyrosequencing pbr322 pcd program cell death apoptosis cry gene plant transformation technology agroinfection biolistic bombardment gene gun microinjection disease resistance plant nematode resistant resistant plant production agrobacterium tumifacience agro infection pr protein chitinase gluconase lysozyme thionins defensins feature of ti plasmid vir regin orign of repication opine reporter gene gus lux f2 luc gfp production of resistant plant pisa nappa dapa types of protein array application of protein array ethics in animal cell culture types of protein protein interaction ethical issue related to animal biotechnology equipment and material for animal cell culture analysis of protein protein interaction glucose other organic study of proteomics proteomic study computational protein interaction genome sequencing shotgun sequencing hierarchical shotgun sequencing whole genome shotgun sequencing random shotgun sequencing salts snp ditection haplotype single base extension differential hybridization gene marker vitamins amino acids osmolality temperature agriculture evolution oxygen production of viruse resistant plant primary cell culture shoutgun method expessed sequence tag ests est types of molecular marker proteomics tools proteome mapping tyes of proteomics cell lines culture primary culture methods of funtional proteomics tools of proteomics proteomic tools gene primary and established cell lines gene and protein primary and established cell line culture mammalian cell culture genetic code structural proteomics human genome project genome project hugo methosds of study of protein study of protein protein database computational analysis of protein basic techniques for animal cell culture basic techniques of mammalian cell culture ethics related to animal