Genetic, developmental, and neural changes underlying the evolution of butterfly mate preference
Fig 3
Differential gene expression between yellow and white Heliconius cydno alithea males.
(A) Stage- and tissue-specific differential expression between yellow and white H. c. alithea males identified using DESeq2 and a global FDR cutoff of 0.05. (B) Genes with significantly different temporal expression patterns in yellow and white H. c. alithea males in each tissue, calculated using maSigPro. Overlap represents genes with significantly different expression profiles in all overlapping tissues, represented by an Euler diagram. maSigPro fits a curve to each gene in each group (i.e., yellow males, yellow females, white male, white females) over all developmental stages. A gene is considered DE if one or more of its regression coefficients is significantly different between yellow and white H. c. alithea males. (C) Expression profiles of DE K locus genes (left), other top choice locus genes (middle), and additional top DE candidate genes (right) (see Figs 2 and S5). AurB is a protein kinase expressed throughout the fly nervous system [45]; Rich is a Rab guanine nucleotide exchange factor required for synapse formation and function from inner photoreceptor cells (fly R7 + R8, butterfly R1/2 + R9) to medulla neurons [41]. The full list of DE genes can be found in S4–S7 Tables.
doi: https://doi.org/10.1371/journal.pbio.3002989.g003