A BRC1-modulated switch in auxin efflux accounts for the competition between Arabidopsis axillary buds
Fig 2
The inhibition of smxl678 buds is rescued by brc1brc2 in 1-node but not in 2-node explants.
(A) PIN1 removal and BRC1 expression phenotype in Col-0, brc1brc2, smxl678, and brc1brc2smxl678. (B) Bud length on 1-node explants measured daily for 10 days. Active buds (blue) are defined as those with a maximum growth rate > 2.5 mm/day, n = 41–72. (C) Number of lag days (number of days before growth rate > 2.5 mm/day) for active buds, n = 41–71 (D) Mitchison plots of top vs. bottom bud lengths on 2-node explants, measured daily for 10 days. The final length of buds is shown with a blue dot, n = 33–67. (E) Percentage of active explants (explants with at least one active bud) from three experimental repeats (where only one percentage is indicated, it is the same across the repeats). Below, relative growth index (RGI) of active explants at day 10. The RGI is defined as the length of the longest bud divided by the total length of both buds. The orange dot indicates the mean, n = 15–58). Letters represent statistically significant differences for p < 0.05 from a multi-level model of three experimental replicates with pairwise comparisons and Bonferroni correction. Data and analysis underlying this figure can be found at https://doi.org/10.17863/CAM.120831.
doi: https://doi.org/10.1371/journal.pbio.3003395.g002