A BRC1-modulated switch in auxin efflux accounts for the competition between Arabidopsis axillary buds
Fig 5
Variation in two model parameters, representing BRC1 expression and PIN1 removal, captures variation in the duration of the bud activation lag phase on 2-node explants for a range of genotypes and treatments.
(A) Heatmap of the number of lag time steps for 25,281 combinations of parameters v0 and μ (159 × 159 grid). The pixel color intensity reflects the mean number of lag days from 50 simulations run for 120 time steps. The genotypes/treatments are assigned the same parameter values as in Fig 4B (iii and iv). (B) Boxplot of the simulated number of lag time steps calculated for each of the genotypes indicated in panel A. The number of lag times steps was calculated from 100 simulations run for 120 time steps. (C) Number of lag days calculated as the number of days before growth rate > 2.5 mm/day for the same dataset as Fig 4A. In the left hand plot, buds were measured for 12 days, except smxl678 which was measured for 19 days. In the right hand plot, all buds were measured for 10 days, n = 12–72. Below, the percentage of active explants, defined as explants with at least one active bud, n = 43–74. The series of genotypes/treatment with a gradual increase in BRC1/v0 and PIN1 removal/µ are shown in light blue. Data and simulations are shown for the longest bud of active explants. Letters represent statistically significant differences for p < 0.05 from a multi-level model with pairwise comparisons and Bonferroni correction. Data and scripts of simulations underlying this figure can be found at https://doi.org/10.17863/CAM.120831.
doi: https://doi.org/10.1371/journal.pbio.3003395.g005