Connective auxin transport contributes to strigolactone-mediated shoot branching control independent of the transcription factor BRC1
Fig 2
Loss of PIN347 reduces stem auxin transport in strigolactone mutants without affecting PIN1.
(A) Bulk stem auxin transport in basal inflorescence stem internodes of 6-week old plants of the genotypes indicated. Transport was determined as basal accumulation of radiolabeled auxin, quantified as counts per minute (CPM) after 6-hours incubation in 1 μM 14C-IAA. (B) Quantification of PIN1-GFP in arbitrary units (A.U) at the basal plasma membrane of xylem parenchyma cells in longitudinal hand sections through basal inflorescence internodes of 6-week old plants of the genotypes indicated, homozygous for PIN1::PIN1-GFP. (C) Quantification of PIN3-GFP, PIN4-GFP and PIN7-GFP in arbitrary units (A.U.) at the basal plasma membrane of xylem parenchyma cells in longitudinal hand sections through basal inflorescence internodes of 6-week old max2 plants. The boxes span the first to third quartile and the line represents the median. The whiskers indicate the variability outside the upper and lower quartiles and outliers are indicated by individual points. Tukey’s HSD test were carried out after obtaining the least-square means for a linear model fitting the data and different letters indicate statistically significant differences at p < 0.05. For (A), n = 19–24. For B and C statistical analyses were carried our using the mean of 5 membranes from 4–8 plants per line.
doi: https://doi.org/10.1371/journal.pgen.1008023.g002