Connective auxin transport contributes to strigolactone-mediated shoot branching control independent of the transcription factor BRC1
Fig 4
ABCB19 contributes to stem auxin transport.
(A) Bulk stem auxin transport through 15 mm basal inflorescence internodes of 6-week old Col-0 and abcb19 plants. Transport was determined as basal accumulation of radiolabeled auxin, quantified as counts per minute (CPM) in the basal 5 mm of stem after 18 hours of incubation of the apical end 1 μM 14C-IAA. (B) Progression of a 10-minute pulse of 5 μM 14C-IAA applied to the apical end of 24 mm long basal inflorescence internodes of 6-week old Col-0 and abcb19 plants assessed at 30, 60 and 90 minutes after application of the pulse (left to right), shown as mean CPM in 2 mm sections of the stems. For A the boxes span the first to third quartile and the line represents the median. The whiskers indicate the variability outside the upper and lower quartiles and outliers are indicated by individual points. Tukey’s HSD test was carried out after obtaining the least-square means for a linear model fitting the data and different letters indicate statistically significant differences at p < 0.05, n = 24. For B the error bars represent the 95% confidence interval of the mean, n = 8 for each point. No significant differences were detected at any time point (unadjusted Wilcoxon rank-sum tests).
doi: https://doi.org/10.1371/journal.pgen.1008023.g004