Pseudomonas aeruginosa Pili and Flagella Mediate Distinct Binding and Signaling Events at the Apical and Basolateral Surface of Airway Epithelium
Figure 4
P. aeruginosa internalization at the AP surface of polarized epithelium is mediated by Tfp-dependent binding to N-glycans and subsequent PI3K activation and, at the BL surface, by flagella-dependent binding to HS and subsequent EGFR/PI3K activation.
Calu-3 cells were grown as well polarized monolayers on Transwells for 9 days and treated with heparin, heparinase III (hepIII), mannose (Man), tunicamycin (tun), EGFR inhibitor (AG1478), PI3K inhibitor (LY29004), or in combination. (A) PAO1, (B) PAO1ΔpilA or (C) PAO1ΔfliC were added to the AP or BL chamber for 2 h and standard invasion assays were performed. Shown is the mean +/− SD for 4 independent experiments. *P<0.05 compared to cells infected with PAO1 at the AP surface (black bar). **P<0.05 compared to cells infected with PAO1 at the BL surface (black bar).
doi: https://doi.org/10.1371/journal.ppat.1002616.g004