VE-Cadherin Cleavage by LasB Protease from Pseudomonas aeruginosa Facilitates Type III Secretion System Toxicity in Endothelial Cells
Figure 8
E-cadherin is resistant to LasB proteolysis.
Epithelial A549 cells were treated with PAO1 or PAO1ΔxcpR secretomes (10% v/v in culture medium). LB was used as control. Proteins were extracted at different time points p.i., as indicated, and analyzed by Western blot with anti-E-cadherin and anti-ß-actin antibodies. E-cadherin was stable and no degradation products were observed. The averaged signal ratios (n = 3) ± SD of E-cadherin (125 kDa) over ß-tubulin are indicated below each lane.
doi: https://doi.org/10.1371/journal.ppat.1003939.g008