functional system of the cell
- 1. Functional systems of the cell Prepared by Fatima Sundus
- 2. If a cell is to live and grow and reproduce, it must obtain nutrients and other substances from the surrounding fluids. Most substances pass through the cell membrane by diffusion and active transport
- 3. Diffusion diffusion is the movement of molecules from a region of higher concentration to a region of lower concentration down the concentration gradient.
- 6. Very large particles enter the cell by a specialized function of the cell membrane called endocytosis. The principal forms of endocytosis are 1. pinocytosis 2. phagocytosis.
- 7. Pinocytosis means ingestion of minute particles that form vesicles of extracellular fluid and particulate constituents inside the cell cytoplasm. Phagocytosis means ingestion of large particles, such as bacteria, whole cells, or portions of degenerating tissue.
- 8. Pinocytosis. • Pinocytosis occurs continually in the cell membranes of most cells For example, it occurs so rapidly in macrophages • Pinocytosis is the only means by which most large macromolecules, such as most protein molecules, can enter cells.
- 10. • molecules attach to cell- surface receptors concentrated in clathrin- coated pits • receptor binding induces invagination • ATP-dependent and involves recruitment of actin and myosin
- 11. Phagocytosis. • it involves large particles rather than molecules. • Only certain cells have the capability of phagocytosis, • most notably the tissue macrophages and white blood cells
- 12. • Phagocytosis is initiated when a particle such as a bacterium,a dead cell, or tissue debris binds with receptors on the surface of the phagocyte. • In the case of bacteria, each bacterium is usually already attached to a specific antibody, and it is the antibody that attaches to the phagocyte receptors, dragging the bacterium along with it. This intermediation of antibodies is called opsonization
- 15. Phagocytosis occurs in the following steps: 1. The cell membrane receptors attach to the surface ligands of the particle. 2. The edges of the membrane around the points of attachment evaginate outward to surround the entire particle 3. Actin and other contractile fibrils in the cytoplasm surround the phagocytic vesicle and contract around its outer edge, pushing the vesicle to the interior 4. The contractile proteins then pinch the stem of the vesicle so completely that the vesicle separates from the cell membrane
- 16. Pinocytotic and Phagocytic Foreign Substances are digested Inside the Cell by Lysosomes after a pinocytotic or phagocytic vesicle appears inside a cell, lysosomes become attached to the vesicle and empty their acid hydrolases to the inside of the vesicle
- 18. Regression of Tissues and Autolysis of Damaged Cells • regression occurs in the uterus after pregnancy, • in muscles during long periods of inactivity, • in mammary glands at the end of lactation. Functions of lysosomes
- 19. Autolysis. • the destruction of cells or tissues by their own enzymes, especially those released by lysosomes. • The lysosomes also contain bactericidal agents that can kill phagocytized bacteria before they can cause cellular damage. These agents include (1) lysozyme, which dissolves the bacterial cell membrane; (2) lysoferrin, which binds iron and stops bacterial growth (3) acid at a pH of about 5.0, which activates the hydrolases and inactivates bacterial metabolic systems.
- 20. Recycling of Cell Organelles—Autophagy. Lysosomes play a key role in the process of autophagy, which literally means “to eat oneself”
- 22. Processing of Endoplasmic Secretions by the Golgi Apparatus—Formation of Vesicles.
- 23. ATP production Step 1. • Carbohydrates are converted into glucose • Proteins are converted into amino acids • Fats are converted into fatty acids Step 2. • Glucose, AA, and FA are processed into AcetylCoA Step 3. • AcetylCoA reacts with O2 to ATP NOTE a maximum of 38 molecules ATP are formed per molecule of glucose degraded
- 25. Functional Characteristics of ATP
- 26. ATP is a nucleotide composed of (1) the nitrogenous base adenine (2) the pentose sugar ribose (3) three phosphate radicals. • The last two phosphate radicals are connected with the remainder of the molecule by so-called high-energy phosphate bonds • Under the physical and chemical conditions of the body, each of these high energy bonds contains about 12,000 calories of energy per mole of ATP
- 27. • When ATP releases its energy, a phosphoric acid radical is split away and adenosine diphosphate (ADP) is Formed • To reconstitute the cellular ATP as it is used up, energy derived from the cellular nutrients causes ADP and phosphoric acid to recombine to form new ATP • For these reasons, ATP has been called the energy currency of the cell.
- 28. Chemical Processes in the Formation of ATP—Role of the Mitochondria. Upon entry into the cells, glucose is subjected to enzymes in the cytoplasm that convert it into pyruvic acid (a process called glycolysis). A small amount of ADP is changed into ATP by the energy released during this conversion, but this amount accounts for less than 5 percent of the overall energy metabolism of the cell
- 29. • About 95 percent of the cell’s ATP formation occurs in the mitochondria. • The pyruvic acid derived from glycolysis converted into the compound acetyl-coenzyme A (CoA) in the matrix of mitochondria.
- 30. acetyl-coenzyme A (CoA) is further dissoluted in the mitochondrion matrix, undergoing dissolution in a sequence of chemical reactions called the citric acid cycle, or Krebs cycle.
- 31. • In this citric acid cycle, acetyl-CoA is split into its component parts, hydrogen atoms and carbon dioxide. The carbon dioxide diffuses out of the mitochondria and eventually out of the cell; finally, it is excreted from the body through the lungs. • The hydrogen atoms, conversely, are highly reactive, and they combine with oxygen that has also diffused into the mitochondria. • This combination releases a tremendous amount of energy, which is used by the mitochondria to convert large amounts of ADP to ATP • This overall process for formation of ATP is called the chemiosmotic mechanism of ATP formation.
- 33. Uses of ATP for Cellular Function. Energy from ATP is used to promote three major categories of cellular functions: (1) transport of substances through multiple membranes in the cell (2) synthesis of chemical compounds throughout the cell (3) mechanical work.
- 34. 1) To supply energy for the transport of sodium potassium ions, calcium ions, magnesium ions, phosphate ions, chloride ions, urate ions, hydrogen ions through the cell membrane (2) to promote protein synthesis by the ribosomes phospholipids, cholesterol, purines, pyrimidine 3) to supply the energy needed during muscle contraction, ciliary and ameboid motion
- 35. More than 95 percent of this ATP is formed in the mitochondria, which accounts for the mitochondria being called the “powerhouses” of the cell.