Sydney Kushner Seminar
- 1. Eduardo E. Rivera Dr. Sydney Kushner Polyadenylation helps Regulate FuntiontRNA Levels in Escherichia Coli Sidney Kushner who came from the University of Georgia offered a seminar titled “Polyadenylation Helps Regulate Function tRNA Levels in Escherichia Coli.” The two important terms in the seminar were polyadenylation and how do you make functional tRNA in Escherichia coli. He explained basic biological terms like the definition of polyadenylation which is the addition of a poly(A) tail to an RNA molecule. tRNA are unusual because after they are made they are modified. There are around 30-35 modifications. One of the modifications was inosine, which I found very interesting. Inosine is actually adenasine denominated and is one of the post - transcriptional modifications made in tRNA. Using a wild type E. coli, they separated four tRNA into smaller units using enzymes. They were comparing what they saw in E. coli and in eukaryotes. When they saw that both E. coli and eukaryotes did not look the same in the gel, they thought that if they eliminated enzymes, (which break the tRNA), it would change the results. In conclusion, they found that there is a competition in the enzymes used to break the tRNA. On the other hand, polyadenalyation is effective in making and regulating a tRNA in E. Coli. These findings can help to understand better the functional levels within the tRNAs.