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1/23/2011C2 BioTechnologies, LLC1By Larry Cosenza
1/23/2011C2 BioTechnologies, LLC2Business OverviewR&D: Design enzymes for industry.Goal: Manufacture enzymes for production of renewable energy.Technology: Fusion Enzyme.Seeking: Licensing and Commercial Partner.
What We DoDesign enzymes for industrial process.Design and construct more robust enzymes.Design and construct enzymes to consolidate industrial bio-process.1/23/2011C2 BioTechnologies, LLC3
How Does This Help?Robust enzymes can reduce variability in process… increasing product yield.Consolidating bio-process reduces operating and consumables costs.Each prototype fusion enzyme can be re-engineered: result in families of products.1/23/2011C2 BioTechnologies, LLC4
1/23/2011C2 BioTechnologies, LLC5RenewableFeedstockWhere does C2 Biotechnologies fit in?Energy
1/23/2011C2 BioTechnologies, LLC6What are Enzymes?Enzymes are proteins, which are produced by all living organisms. Enzymes are responsible for a number of biological activities in plants, animals, human beings and microorganisms.Enzymes are distinguishable from other proteins because of their catalytic activity.Enzymes work by making or breaking chemical bonds.
1/23/2011C2 BioTechnologies, LLC7How Do Enzymes Work?Gluco-amylasea - Amylase
First Product PrototypeAmylase Fusion EnzymeMore robust Active over wide range temperatureActively converts starch into glucoseSimple bacterial production systemTarget markets: ethanol, corn sweetener, fermentation.1/23/2011C2 Biotechnologies, LLC8
Second Technology in DevelopmentCellulase Fusion EnzymeConsolidate bio-processSBIR funded by Department of EnergyTarget market: cellulosic ethanol1/23/2011C2 BioTechnologies, LLC9
1/23/2011C2 BioTechnologies, LLC10Industrial Enzymes MarketCurrent Enzyme Market $3.3 billion.Currently ~13 billion gallons ethanol produced in U.S. nearly all from corn.  Global market expected to grow. Amylase market ~20% of total or ~$660 million.Cellulase market: growing…
1/23/2011C2 BioTechnologies, LLC11Industrial Enzyme Pain PointsC2 BioTechnologiesFits in here.
1/23/2011C2 BioTechnologies, LLC12C2BioTechnologies:Right Time Right PlaceFederal & State legislation mandating use of bio-fuels.Current enzyme market growing.New technology / new products.New markets.Fusion technology addresses industry pain points.
SummaryR&D : Design and Construct Fusion Enzyme Technology for Industrial Use.Products: Amylase & Cellulase Fusion TechnologiesTarget Markets: Energy, Food, Fermentation.Goal: Manufacture Enzymes.Seeking: Licensing and Commercial Partner.1/23/2011C2 BioTechnologies, LLC13

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Web Presentation

  • 2. 1/23/2011C2 BioTechnologies, LLC2Business OverviewR&D: Design enzymes for industry.Goal: Manufacture enzymes for production of renewable energy.Technology: Fusion Enzyme.Seeking: Licensing and Commercial Partner.
  • 3. What We DoDesign enzymes for industrial process.Design and construct more robust enzymes.Design and construct enzymes to consolidate industrial bio-process.1/23/2011C2 BioTechnologies, LLC3
  • 4. How Does This Help?Robust enzymes can reduce variability in process… increasing product yield.Consolidating bio-process reduces operating and consumables costs.Each prototype fusion enzyme can be re-engineered: result in families of products.1/23/2011C2 BioTechnologies, LLC4
  • 5. 1/23/2011C2 BioTechnologies, LLC5RenewableFeedstockWhere does C2 Biotechnologies fit in?Energy
  • 6. 1/23/2011C2 BioTechnologies, LLC6What are Enzymes?Enzymes are proteins, which are produced by all living organisms. Enzymes are responsible for a number of biological activities in plants, animals, human beings and microorganisms.Enzymes are distinguishable from other proteins because of their catalytic activity.Enzymes work by making or breaking chemical bonds.
  • 7. 1/23/2011C2 BioTechnologies, LLC7How Do Enzymes Work?Gluco-amylasea - Amylase
  • 8. First Product PrototypeAmylase Fusion EnzymeMore robust Active over wide range temperatureActively converts starch into glucoseSimple bacterial production systemTarget markets: ethanol, corn sweetener, fermentation.1/23/2011C2 Biotechnologies, LLC8
  • 9. Second Technology in DevelopmentCellulase Fusion EnzymeConsolidate bio-processSBIR funded by Department of EnergyTarget market: cellulosic ethanol1/23/2011C2 BioTechnologies, LLC9
  • 10. 1/23/2011C2 BioTechnologies, LLC10Industrial Enzymes MarketCurrent Enzyme Market $3.3 billion.Currently ~13 billion gallons ethanol produced in U.S. nearly all from corn. Global market expected to grow. Amylase market ~20% of total or ~$660 million.Cellulase market: growing…
  • 11. 1/23/2011C2 BioTechnologies, LLC11Industrial Enzyme Pain PointsC2 BioTechnologiesFits in here.
  • 12. 1/23/2011C2 BioTechnologies, LLC12C2BioTechnologies:Right Time Right PlaceFederal & State legislation mandating use of bio-fuels.Current enzyme market growing.New technology / new products.New markets.Fusion technology addresses industry pain points.
  • 13. SummaryR&D : Design and Construct Fusion Enzyme Technology for Industrial Use.Products: Amylase & Cellulase Fusion TechnologiesTarget Markets: Energy, Food, Fermentation.Goal: Manufacture Enzymes.Seeking: Licensing and Commercial Partner.1/23/2011C2 BioTechnologies, LLC13