Learning Set 3: Lesson 1
- 1. Proteins Move Based on Size lactase tyrosinase Learning Set 3 : Lesson 1 : Slide 1
- 2. Analyzing LDL receptor A B C LDL receptor Why did these LDL receptor proteins move Normal LDL Receptors farther down the columns? George’s LDL Receptors Learning Set 3 : Lesson 1 : Slide 2
- 3. What Is The Difference Here? Sequence of amino acids in LDL receptor protein: Column A MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQF WRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDC EDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDC KDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLV AQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTE VASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIV VDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSL AVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPD GMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVR ALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDV Column B VCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTL DRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADT KGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVN GGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQY LCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSH QALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLED DV Column C MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQF WRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDC EDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDC KDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLV AQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTK Learning Set 3 : Lesson 1 : Slide 3
- 4. DNA Location Learning Set 3 : Lesson 2 : Slide 4
- 5. Chromosomes Learning Set 3 : Lesson 2 : Slide 5
- 6. Pieces to DNA Model 6 T (Orange) 1 base 12 spacers 1 cap 6 A (Blue) (light brown) (transparent) (white) 6 G (Green) 6 C (Yellow) 1 rod (grey) 2 side chains composed of alternating deoxyribose (red) and phosphate (purple) Learning Set 3 : Lesson 3 : Slide 6
- 7. DNA Base Pairs Given DNA Matching DNA Strand T G G C G C T G T G Learning Set 3 : Lesson 3 : Slide 7
- 8. Pieces to LDL Receptor Protein DNA Model Learning Set 3 : Lesson 3 : Slide 8
- 9. Marshmallow Color Key Marshmallow Color Chemical Base Orange Thymine (T) Pink Adenine (A) Green Guanine (G) Yellow Cytosine (C) Learning Set 3 : Lesson 3 : Slide 9
- 10. Step by Step Pictures Step #1: Learning Set 3 : Lesson 3 : Slide 10
- 11. Step by Step Pictures (cont.) Step #2: Learning Set 3: Lesson 3: Slide 11
- 12. Step by Step Pictures (cont.) Step #3: Learning Set 3 : Lesson 3 : Slide 12
- 13. Chromosomes to DNA to Genes How small is small? Learning Set 3 : Lesson 4 : Slide 13
- 14. From DNA to Proteins Transcription -Translation Learning Set 3 : Lesson 4 : Slide 14
- 15. From DNA to Proteins 1 Transcription & Translation Overview Learning Set 3 : Lesson 4 : Slide 15
- 16. Symbols for Transcription - Translation Models Learning Set 3 : Lesson 4 : Slide 16
- 17. DNA vs. RNA DNA RNA Learning Set 3 : Lesson 4 : Slide 17
- 18. DNA vs mRNA DNA RNA Learning Set 3 : Lesson 4 : Slide 18
- 19. From DNA to Proteins 2 Transcription & Translation Interactive Modeling Learning Set 3 : Lesson 4 : Slide 19
- 20. Coding Amino Acids RNA amino acid Learning Set 3 : Lesson 4 : Slide 20
- 21. LDL Receptor Learning Set 3 : Lesson 4 : Slide 21
- 22. Where are our genes? Learning Set 3 : Lesson 4 : Slide 22
- 23. Location of LDL Receptor Gene Learning Set 3 : Lesson 4 : Slide 23
- 24. LDL Receptor Gene Segment focused on in class Learning Set 3 : Lesson 3 : Slide 24
- 25. Marshmallow DNA Model AC C G C GACAC Learning Set 3 : Lesson 4 : Slide 25
- 26. RNA Marshmallow Color Key Marshmallow Color Chemical Base White Uracil (U) Pink Adenine (A) Green Guanine (G) Yellow Cytosine (C) Learning Set 3 : Lesson 4: Slide 26
- 27. Coding Amino Acids RNA amino acid Learning Set 3 : Lesson 4 : Slide 27
- 28. Reviewing George’s Symptoms Familial Hypercholesterolemia (FH) • Very high cholesterol in blood • Waxy patches on the skin • Chest pain and heart attacks at a young age • Build up of fatty deposits on under skin and in arteries Knees and Fingers Coronary Artery: a heart artery Fatty Deposit Fatty Deposit 28 Learning Set 3 : Lesson 5 : Slide 28
- 29. George’s Family History George Heart attack: 25y Learning Set 3 : Lesson 5 : Slide 29
- 30. Why George had a Heart Attack Fatty Deposits Cross Section of In Coronary Artery Coronary Artery Tear in Normal artery wall Fatty Deposit Fatty Narrowed deposits artery made in blocked by artery blood clot wall • Fatty deposits made in artery wall, high blood pressure • Arteries harden and narrow due to fat accumulation • Blood flow is reduced • Oxygen supply to heart reduced • Can cause chest pain heart attack or death in severe cases 30 Learning Set 3 : Lesson 5 : Slide 30
- 31. Cholesterol: Many Roles Cholesterol Estrogen Testosterone Bile Acids 31 Learning Set 3 : Lesson 5 : Slide 31
- 32. Low density lipoprotein (LDL) Receptor cholesterol Cholesterol in LDL LDL Receptor outside cell Plasma Membrane cell inside cell Takes LDL into cell nucleus 32 Learning Set 3 : Lesson 5 : Slide 32
- 34. Analyzing George’s LDL receptor A B C LDL receptor George’s LDL receptor 34 Learning Set 3 : Lesson 5: Slide 34
- 35. Why did Rachel look at George’s DNA? One of George’s chromosomes, chromosome 19, had an arrow pointing at one spot on it. This arrow indicated something was different at this spot on chromosome 19. What does DNA have to do with the LDL receptor that causes FH disease? 35 Learning Set 3 : Lesson 5 : Slide 35
- 36. Analyzing the Family’s DNA George 36 Learning Set 3 : Lesson 5 : Slide 36