4rth summary rise slideshare
- 1. 4rth Summary- Water Sampling and Testing and Dr. Javier Arce February 28, 2014 Coliphages are bacteriophages that infect coliforms (E. coli). They are microbiological indicators, namely microorganisms whose presence determine whether or not pathogens are present. They are commonly used to indicate if water is polluted by fecal coliforms. The objective of this investigation is to examine water samples for pollution due to animal fecal matter brought from different areas. There are different common water sampling procedures to indicate pollution such as indicator organisms, fecal indicators and molecular identification of indicator organisms. Nonetheless, these procedures have their faults and disadvantages, which is why the coliphage method is used. This method is more efficient since it is fast, there is no need for confirmation tests, it is cost-effective and it is a simple procedure. The purpose of this research is to compare distilled water with non-treated water to confirm the efficiency of water treatments using the coliphage methodThis method will prove whether or not these water treatments are efficiently reducing or eliminating animal fecal matter from water. In this method, E.coli are used since they are ideal for indicating the presence of coliphages. To perform this method, a tryptic soy broth (TSB), used as a growth media for the culturing of bacteria, and an agar flask are removed from the water bath at 60°C. Using aseptic technique, 2 mL of E. coli and 100mLof the water sample are added to the 100 mL of TSB and agar flask when the temperature drops slightly. Finally, 8 empty labeled petri dishes are filled with the solution before it solidifies and until the bottom of each dish is completely filled. Next they are incubated at 36° C. After 24 hours of incubation, there were no microbiological indictors in the dishes, meaning that the water samples showed no fecal contamination, hence there were no phages, and results were negative. Coliphages are used to reveal water quality by detecting bacteria and viruses in water that can cause illnesses. By using these bacteriophages, water quality can be identified in water sources, and it can prevent diseases caused by water pollution.