![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Investigating the functional significance of corticospinal and thalamocortical
neurons in learning and performance of complex behavioral tasks in the adult
mammalian motor system
Abstract
Contrary to popular perception, our brains are not simply static systems that process and respond to
external stimuli, but are constantly adapting themselves in a dynamic fashion. One area of the brain that
has been an ideal model for studying learning and associated brain plasticity is the primary motor cortex.
In this study, our goal is to evaluate how distinct populations of neurons in the thalamus and primary
motor cortex contribute to learning and performance of skilled motor tasks in rodents. Thalamocortical
inputs to the motor cortex are postulated to relay information from a variety of nuclei that mediate and
coordinate motor performance, including the basal ganglia and cerebellum. Corticospinal neurons are the
sole source of neuronal output to the spinal cord from the cortex and are postulated to play a direct role in
skilled motor learning in rats. Prior studies attempting to define the function of these neuronal systems in
skilled motor behavior have involved ablation strategies that resulted in damage to non-target cell
populations, thus confounding the interpretation of experimental findings. The goal of the present study
is to use a novel viral approach to selectively eliminate distinct populations of neurons implicated in
motor function and examine subsequent changes in motor performance. It is postulated that behavioral
changes will occur that restrict proper task performance and inhibit motor adaptation.
Above is the condensed form of the project paper that was produced after completing the first
round of experiments performed on subject rats during the summer portion of my research. I will also
provide a copy of the introduction of the research paper which includes, in my words, an explanation of
why we selected to perform a thalamocortical ablation.
I will briefly describe the severalsteps and procedures performed during the development and
analysis of this project. These steps, outlined, are as follows: train rats in forelimb reaching task, inject
toxin, repeat forelimb task, perform further behavioral assays,perfuse rats, remove brain and slice with
microtome, perform immunohistochemistry, analyze ablation, collect and analyze data.
As a MARC scholar I was granted the opportunity to be an intern in the Tuszynski lab, UCSD
Center for NeuralRepair, under the supervision of Dr. James Conner, associate project scientist . My
research experience began with a brief overview of the project and a journal filled folder. I was instructed
to read and analyze the papers and to speculate the outcome of the project. The first two weeks of the
project consisted of training, familiarization with the protocol and required techniques, and reading
roughly 30 scientific journals. My room decorated with the contents of that folder and my whiteboard
representing the intricate connections of the brain and culminated propositions; I was determined to have
the correct answer.
I first learned how to use a microtome. This tool is used to slice 40 micrometer brain sections that
are later mounted on a stage. Spare brains were used for training and soon enough I was confident in my
ability to use this machine. Next, the slices were collected and stained using immunohistochemistry.
During this process,the tissue is stained with antibodies that, once attached to a tagged tracer,expose the
effects of the toxin used to ablate the desired brain cells.
After mastering the complexity of tissue processing I progressed to learn rat training. Tis
required completion of VA medical hospital required training and paperwork; a process that takes up to
three weeks. During this training session I learned how to care for,acclimate, and train the rat subjects
used in almost every lab.](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-1-320.jpg)
![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Every day, for about 8 hours a day, I would meet with Dr. Conner and assist with training. After
training, the rats were surgically injected with the toxin. I was in charge of re-training the rats, post-
surgery, in the forelimb reaching task. A series of behavioral assays were also performed in order to
obtain a better understanding akin to the neurological difficulties observed.
The data was recorder,graphed, and analyzed. Furthermore, video footage was obtained in order
to analyze each movement, frame by frame,with an attempt to pinpoint the potential nuclei that was
malfunctioning. Hours were spent discussing potential candidates and formulating ideas and explanations
that would then be justified by tissue processing.
Once training and data collection was finalized, the animals were perfused and tissue processing
began. Eighteen rats were processed and we were able to see the outcome of the ablation roughly 2
months after the project began. This information was gathered and synthesized into an abstract and final
project paper.
Over summer I worked 40 hours a week to assist with this great research project. I currently still
work with Dr. Conner and the project has moved forward to analyze different connections akin the
network of neurons that partake in the forelimb reaching task. The lab has adopted new techniques that
allow researcher to inhibit or excite a specific population of target neurons by use of a designer ligand that
works on a novel receptor. The use of this technique has just recently been introduced and I look forward
to observing the results and performing behavioral assays. In this manner we can observe behavioral
deficits that arise from both the loss and the strengthening of the thalamacortical tract. Aside from the
thalamocortical and corticospinal tract, we will continue to examine connections within the network that
correlates to the forelimb reaching task. Our next goal will be to analyze the connection that channels
coordination and motor control from the cerebellum to the thalamus by performing a dentate-thalamic
ablation.](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-2-320.jpg)
![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Logistics
Purpose: To presentandexplainthe procedure andoutcome of researchconductedunderthe
supervisionof Dr.JamesConner.Thisopportunitywill strengthenmyabilityasa scientistto
create and organize a presentationandtocommunicate the complexityof thisprojectinaclear
and consistmanner.
Conference:EmergingResearchersNational Conference inSTEM February20-22, 2014
Location: WashingtonD.C
Contact: Donna Behar,ERN coordinator- dbehar@aaas.org
Conference description: The ERN conference isdesignedtopromote scientificenrichment.
Topicsin STEM converge tocreate an experience that,Ibelieve,willstrengthenandenrichmy
scientificcareer. Conferenceeventsinclude: Plenarysession,guestspeakers,poster
presentations,oral presentations,exhibitions,graduate studentpresentations,andnetworking
events.
Budget
Expenses Expense Detail Expense Detail Total
Flight - I will leave on Thursday night the 20th
and return Sunday afternoon, the 23rd
Airfare quoted amount: $557.91
USD
Taxes and fees:$85.84 USD
$643.75 USD
Conference registration fee -Confirmation Number: M7NZDY7NJ56 $400 USD
Lodging -Four Points by Shareton -$107/night + Taxes
-Friday + Saturday night $245.49 USD
Transportation -Taxi: UCSD to San Diego- Lindbergh Intl
Arpt (SAN)
-Taxi: Lindbergh Intl Arpt (SAN) to UCSD
to San Diego
-Taxi: USD Ronald Reagan National Arpt
(DCA) to Hotel (1201 K St NW Washington,
DC 20005)
-Taxi: USD Hotel to airport
-Bus transportation:
From hotel to conference
– $49.65
15 miles
– $49.65
15 miles
– $24.17
4.9 miles
-$24.17
4.9 miles
-$2.42 USD/ trip
$9.68 USD expected
$147.64 USD
Food -Saturday- Sunday Breakfast, Lunch and
dinner
-Washington D.C average
cost/meal $15 USD ( $11-$20
USD)
$120 USD
Poster Printing -$40 USD $40 USD
Total $ 1436.88 USD](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-3-320.jpg)
![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Introduction
Neuroplasticity is the antagonist of the once held dogma; that the brain, once fully developed,
remains stagnant in its ways and resilient to change. It is know now, however, that behavioral
changes and learning elicit new synaptic connections that rearrange neural pathways. This
phenomenon occurs throughout the entirety of the brain which makes it difficult to study.
However, we can isolate this phenomenon by observing changes that occur in the brain due to
motor learning.
The basal ganglia and cerebellum are two important brain regions necessary for learning and
motor performance. These brain regions do not communicate directly with primary motor cortex
but relay information to the primary motor cortex (M1) by way of the ventral lateral and ventral
anterior (Va/Vl)nuclei of thalamus (Cruikshank et al 2010). The basal ganglia has been
postulated to play a role in motivation, motor control, gain control, and motor learning. The
cerebellum corrects and plans motor commands and coordinates proper balance (Kaneko et al
2009). Output from these distinct brain centers converges onto Va/Vl and is then relayed to
primary motor cortex where thalamocortical inputs terminate monosynapticly onto corticospinal
motor neurons in Layer VB.
While prior studies ascertain the general function of these brain regions, the complexity and
significance of each individual connection that collaborates in the overall circuit of each brain
region, be it cerebellum or basal ganglia, remains incomplete.
Prior studies rely on electrolytic or cytotoxic lesions that not only damage the target but also
elicit collateral damage to surrounding areas Thus, a significant problem which stems from such
lesions—difficult as it may be to target a specific area in the brain, it is even more difficult to
target a specific connection and analyze a circuit in the brain.
The present experiment makes use of a novel method that allows for highly selective ablations of
a specific connection in the brain. A rabies pseudotyped lentivirus is used to retrogradely infect
cells providing afferent innervation of a specific brain region with a foreign receptor, the human
form of the IL2 receptor. Later, an immonotoxin selectively targeting the human IL2 receptor is
used to mediate selective ablation of one or more of the infected cell populations.
Using this technique we can hypothesize and observe the functional significance of the
thalamocortical tract akin to a complex motor behavior such as distal forelimb reach and
dexterity. In this manner, learning and performance related brain regions can be disassembled
and analyzed in order to ascertain the origins of motor learning and task performance.
Hypothesis: It is postulated that a thalamocortical ablation should result in behavioral
irregularities parallel to neurological disorders such as Parkinson’s and Huntington’s disease
ataxia, tremors, and dysdiadochokinesis, respectively. Therefore an overall decline in forelimb
reaching performance, inability to adapt to task altercations, and uncoordinated movements are
expected.](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-4-320.jpg)
![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Trip Overview
Trip Name: Trip from San Diego to Washington (For Christian Jaques Hissom )
Start Date: Feb 20, 2014
End Date: Feb 23, 2014
Created:Feb 3, 2014, KIRSTEN KUNG (Modified: Feb 3, 2014)
Description: ERN Conference -- Hissom
Please enter 00 and your 6 digit Event Number, the TEPC number, or Personal travel enter 00999999.: 00902655
Purpose of trip: Business
Agency Record Locator: KFGSBF
Passengers:Christian Hissom
Total Estimated Cost: $643.75 USD
Airfare must be ticketed by an agent by: 02/04/2014 11:00 PM Pacific
Balboa Travel (University of California - San Diego campus)
800-682-6170
800-359-8773
Reservations
Thursday, February 20, 2014
Flight San Diego, CA (SAN) to Atlanta, GA (ATL)
Delta 1792
This flight leaves on Feb 20 and arrives on Feb 21.
Departure: 10:50 PM
Seat:25E (Confirmed)
Lindbergh Intl Arpt (SAN)
Terminal:2
Duration: 3 hours, 54 minutes
Nonstop
Arrival: 05:44 AM
Hartsfield Intl Arpt (ATL)
Terminal:SOUTH TERMINAL
Confirmation: HCYHRT
Status: Confirmed
Additional Details
Aircraft: Boeing 737-900 Distance: 1885 miles
E-Ticket
Cabin: Economy (U) Meal: Refreshments for Purchase
Friday, February 21, 2014
1 hr, 36 min layover at Hartsfield Intl Arpt (ATL)
Flight Atlanta, GA (ATL) to Washington, DC (DCA)
Delta 1138
Departure: 07:20 AM
Seat:34A (Confirmed)
Confirmation: HCYHRT
Status: Confirmed](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-5-320.jpg)
![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Hartsfield Intl Arpt (ATL)
Terminal:SOUTH TERMINAL
Duration: 1 hour, 39 minutes
Nonstop
Arrival: 08:59 AM
Ronald Reagan National Arpt (DCA)
Terminal:B
Additional Details
Aircraft: Douglas MD-85 Distance: 547 miles
E-Ticket
Cabin: Economy (U)
Sunday, February 23, 2014
Flight Washington, DC (DCA) to Minneapolis/St. Paul, MN (MSP)
Delta 1763
Departure: 02:35 PM
Seat:22D (Confirmed)
Ronald Reagan National Arpt (DCA)
Terminal:B
Duration: 2 hours, 48 minutes
Nonstop
Arrival: 04:23 PM
Minneapolis St Paul Intl (MSP)
Terminal:TERMINAL 1 - LINDBERGH
Confirmation: HCYHRT
Status: Confirmed
Additional Details
Aircraft: Douglas MD-90 Distance: 928 miles
E-Ticket
Cabin: Economy (X) Meal: Refreshments for Purchase
1 hr, 17 min layover at Minneapolis St Paul Intl (MSP)
Flight Minneapolis/St. Paul, MN (MSP) to San Diego, CA (SAN)
Delta 1389
Departure: 05:40 PM
Seat:37B (Confirmed)
Minneapolis St Paul Intl (MSP)
Terminal:TERMINAL 1 - LINDBERGH
Duration: 3 hours, 51 minutes
Nonstop
Arrival: 07:31 PM
Lindbergh Intl Arpt (SAN)
Terminal:2
Confirmation: HCYHRT
Status: Confirmed
Additional Details
Aircraft: Boeing 757-200 Distance: 1529 miles
E-Ticket](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-6-320.jpg)
![[AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014
Cabin: Economy (X) Meal: Food for purchase
Total Estimated Cost
Air
Airfare quoted amount: $557.91 USD
Taxes and fees: $85.84 USD
Total Estimated Cost: $643.75 USD
Restrictions
Quote: NONREF/PENALTY/APPLIES
TICKET NOT YET ISSUED. AIRFARE QUOTED IN ITINERARY IS NOT GUARANTEED UNTIL TICKETS ARE ISSUED.](https://image.slidesharecdn.com/49f82223-86d2-4607-9c70-7168c6156227-160226180947/85/AS-application-7-320.jpg)
AS application
- 1. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Investigating the functional significance of corticospinal and thalamocortical neurons in learning and performance of complex behavioral tasks in the adult mammalian motor system Abstract Contrary to popular perception, our brains are not simply static systems that process and respond to external stimuli, but are constantly adapting themselves in a dynamic fashion. One area of the brain that has been an ideal model for studying learning and associated brain plasticity is the primary motor cortex. In this study, our goal is to evaluate how distinct populations of neurons in the thalamus and primary motor cortex contribute to learning and performance of skilled motor tasks in rodents. Thalamocortical inputs to the motor cortex are postulated to relay information from a variety of nuclei that mediate and coordinate motor performance, including the basal ganglia and cerebellum. Corticospinal neurons are the sole source of neuronal output to the spinal cord from the cortex and are postulated to play a direct role in skilled motor learning in rats. Prior studies attempting to define the function of these neuronal systems in skilled motor behavior have involved ablation strategies that resulted in damage to non-target cell populations, thus confounding the interpretation of experimental findings. The goal of the present study is to use a novel viral approach to selectively eliminate distinct populations of neurons implicated in motor function and examine subsequent changes in motor performance. It is postulated that behavioral changes will occur that restrict proper task performance and inhibit motor adaptation. Above is the condensed form of the project paper that was produced after completing the first round of experiments performed on subject rats during the summer portion of my research. I will also provide a copy of the introduction of the research paper which includes, in my words, an explanation of why we selected to perform a thalamocortical ablation. I will briefly describe the severalsteps and procedures performed during the development and analysis of this project. These steps, outlined, are as follows: train rats in forelimb reaching task, inject toxin, repeat forelimb task, perform further behavioral assays,perfuse rats, remove brain and slice with microtome, perform immunohistochemistry, analyze ablation, collect and analyze data. As a MARC scholar I was granted the opportunity to be an intern in the Tuszynski lab, UCSD Center for NeuralRepair, under the supervision of Dr. James Conner, associate project scientist . My research experience began with a brief overview of the project and a journal filled folder. I was instructed to read and analyze the papers and to speculate the outcome of the project. The first two weeks of the project consisted of training, familiarization with the protocol and required techniques, and reading roughly 30 scientific journals. My room decorated with the contents of that folder and my whiteboard representing the intricate connections of the brain and culminated propositions; I was determined to have the correct answer. I first learned how to use a microtome. This tool is used to slice 40 micrometer brain sections that are later mounted on a stage. Spare brains were used for training and soon enough I was confident in my ability to use this machine. Next, the slices were collected and stained using immunohistochemistry. During this process,the tissue is stained with antibodies that, once attached to a tagged tracer,expose the effects of the toxin used to ablate the desired brain cells. After mastering the complexity of tissue processing I progressed to learn rat training. Tis required completion of VA medical hospital required training and paperwork; a process that takes up to three weeks. During this training session I learned how to care for,acclimate, and train the rat subjects used in almost every lab.
- 2. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Every day, for about 8 hours a day, I would meet with Dr. Conner and assist with training. After training, the rats were surgically injected with the toxin. I was in charge of re-training the rats, post- surgery, in the forelimb reaching task. A series of behavioral assays were also performed in order to obtain a better understanding akin to the neurological difficulties observed. The data was recorder,graphed, and analyzed. Furthermore, video footage was obtained in order to analyze each movement, frame by frame,with an attempt to pinpoint the potential nuclei that was malfunctioning. Hours were spent discussing potential candidates and formulating ideas and explanations that would then be justified by tissue processing. Once training and data collection was finalized, the animals were perfused and tissue processing began. Eighteen rats were processed and we were able to see the outcome of the ablation roughly 2 months after the project began. This information was gathered and synthesized into an abstract and final project paper. Over summer I worked 40 hours a week to assist with this great research project. I currently still work with Dr. Conner and the project has moved forward to analyze different connections akin the network of neurons that partake in the forelimb reaching task. The lab has adopted new techniques that allow researcher to inhibit or excite a specific population of target neurons by use of a designer ligand that works on a novel receptor. The use of this technique has just recently been introduced and I look forward to observing the results and performing behavioral assays. In this manner we can observe behavioral deficits that arise from both the loss and the strengthening of the thalamacortical tract. Aside from the thalamocortical and corticospinal tract, we will continue to examine connections within the network that correlates to the forelimb reaching task. Our next goal will be to analyze the connection that channels coordination and motor control from the cerebellum to the thalamus by performing a dentate-thalamic ablation.
- 3. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Logistics Purpose: To presentandexplainthe procedure andoutcome of researchconductedunderthe supervisionof Dr.JamesConner.Thisopportunitywill strengthenmyabilityasa scientistto create and organize a presentationandtocommunicate the complexityof thisprojectinaclear and consistmanner. Conference:EmergingResearchersNational Conference inSTEM February20-22, 2014 Location: WashingtonD.C Contact: Donna Behar,ERN coordinator- dbehar@aaas.org Conference description: The ERN conference isdesignedtopromote scientificenrichment. Topicsin STEM converge tocreate an experience that,Ibelieve,willstrengthenandenrichmy scientificcareer. Conferenceeventsinclude: Plenarysession,guestspeakers,poster presentations,oral presentations,exhibitions,graduate studentpresentations,andnetworking events. Budget Expenses Expense Detail Expense Detail Total Flight - I will leave on Thursday night the 20th and return Sunday afternoon, the 23rd Airfare quoted amount: $557.91 USD Taxes and fees:$85.84 USD $643.75 USD Conference registration fee -Confirmation Number: M7NZDY7NJ56 $400 USD Lodging -Four Points by Shareton -$107/night + Taxes -Friday + Saturday night $245.49 USD Transportation -Taxi: UCSD to San Diego- Lindbergh Intl Arpt (SAN) -Taxi: Lindbergh Intl Arpt (SAN) to UCSD to San Diego -Taxi: USD Ronald Reagan National Arpt (DCA) to Hotel (1201 K St NW Washington, DC 20005) -Taxi: USD Hotel to airport -Bus transportation: From hotel to conference – $49.65 15 miles – $49.65 15 miles – $24.17 4.9 miles -$24.17 4.9 miles -$2.42 USD/ trip $9.68 USD expected $147.64 USD Food -Saturday- Sunday Breakfast, Lunch and dinner -Washington D.C average cost/meal $15 USD ( $11-$20 USD) $120 USD Poster Printing -$40 USD $40 USD Total $ 1436.88 USD
- 4. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Introduction Neuroplasticity is the antagonist of the once held dogma; that the brain, once fully developed, remains stagnant in its ways and resilient to change. It is know now, however, that behavioral changes and learning elicit new synaptic connections that rearrange neural pathways. This phenomenon occurs throughout the entirety of the brain which makes it difficult to study. However, we can isolate this phenomenon by observing changes that occur in the brain due to motor learning. The basal ganglia and cerebellum are two important brain regions necessary for learning and motor performance. These brain regions do not communicate directly with primary motor cortex but relay information to the primary motor cortex (M1) by way of the ventral lateral and ventral anterior (Va/Vl)nuclei of thalamus (Cruikshank et al 2010). The basal ganglia has been postulated to play a role in motivation, motor control, gain control, and motor learning. The cerebellum corrects and plans motor commands and coordinates proper balance (Kaneko et al 2009). Output from these distinct brain centers converges onto Va/Vl and is then relayed to primary motor cortex where thalamocortical inputs terminate monosynapticly onto corticospinal motor neurons in Layer VB. While prior studies ascertain the general function of these brain regions, the complexity and significance of each individual connection that collaborates in the overall circuit of each brain region, be it cerebellum or basal ganglia, remains incomplete. Prior studies rely on electrolytic or cytotoxic lesions that not only damage the target but also elicit collateral damage to surrounding areas Thus, a significant problem which stems from such lesions—difficult as it may be to target a specific area in the brain, it is even more difficult to target a specific connection and analyze a circuit in the brain. The present experiment makes use of a novel method that allows for highly selective ablations of a specific connection in the brain. A rabies pseudotyped lentivirus is used to retrogradely infect cells providing afferent innervation of a specific brain region with a foreign receptor, the human form of the IL2 receptor. Later, an immonotoxin selectively targeting the human IL2 receptor is used to mediate selective ablation of one or more of the infected cell populations. Using this technique we can hypothesize and observe the functional significance of the thalamocortical tract akin to a complex motor behavior such as distal forelimb reach and dexterity. In this manner, learning and performance related brain regions can be disassembled and analyzed in order to ascertain the origins of motor learning and task performance. Hypothesis: It is postulated that a thalamocortical ablation should result in behavioral irregularities parallel to neurological disorders such as Parkinson’s and Huntington’s disease ataxia, tremors, and dysdiadochokinesis, respectively. Therefore an overall decline in forelimb reaching performance, inability to adapt to task altercations, and uncoordinated movements are expected.
- 5. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Trip Overview Trip Name: Trip from San Diego to Washington (For Christian Jaques Hissom ) Start Date: Feb 20, 2014 End Date: Feb 23, 2014 Created:Feb 3, 2014, KIRSTEN KUNG (Modified: Feb 3, 2014) Description: ERN Conference -- Hissom Please enter 00 and your 6 digit Event Number, the TEPC number, or Personal travel enter 00999999.: 00902655 Purpose of trip: Business Agency Record Locator: KFGSBF Passengers:Christian Hissom Total Estimated Cost: $643.75 USD Airfare must be ticketed by an agent by: 02/04/2014 11:00 PM Pacific Balboa Travel (University of California - San Diego campus) 800-682-6170 800-359-8773 Reservations Thursday, February 20, 2014 Flight San Diego, CA (SAN) to Atlanta, GA (ATL) Delta 1792 This flight leaves on Feb 20 and arrives on Feb 21. Departure: 10:50 PM Seat:25E (Confirmed) Lindbergh Intl Arpt (SAN) Terminal:2 Duration: 3 hours, 54 minutes Nonstop Arrival: 05:44 AM Hartsfield Intl Arpt (ATL) Terminal:SOUTH TERMINAL Confirmation: HCYHRT Status: Confirmed Additional Details Aircraft: Boeing 737-900 Distance: 1885 miles E-Ticket Cabin: Economy (U) Meal: Refreshments for Purchase Friday, February 21, 2014 1 hr, 36 min layover at Hartsfield Intl Arpt (ATL) Flight Atlanta, GA (ATL) to Washington, DC (DCA) Delta 1138 Departure: 07:20 AM Seat:34A (Confirmed) Confirmation: HCYHRT Status: Confirmed
- 6. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Hartsfield Intl Arpt (ATL) Terminal:SOUTH TERMINAL Duration: 1 hour, 39 minutes Nonstop Arrival: 08:59 AM Ronald Reagan National Arpt (DCA) Terminal:B Additional Details Aircraft: Douglas MD-85 Distance: 547 miles E-Ticket Cabin: Economy (U) Sunday, February 23, 2014 Flight Washington, DC (DCA) to Minneapolis/St. Paul, MN (MSP) Delta 1763 Departure: 02:35 PM Seat:22D (Confirmed) Ronald Reagan National Arpt (DCA) Terminal:B Duration: 2 hours, 48 minutes Nonstop Arrival: 04:23 PM Minneapolis St Paul Intl (MSP) Terminal:TERMINAL 1 - LINDBERGH Confirmation: HCYHRT Status: Confirmed Additional Details Aircraft: Douglas MD-90 Distance: 928 miles E-Ticket Cabin: Economy (X) Meal: Refreshments for Purchase 1 hr, 17 min layover at Minneapolis St Paul Intl (MSP) Flight Minneapolis/St. Paul, MN (MSP) to San Diego, CA (SAN) Delta 1389 Departure: 05:40 PM Seat:37B (Confirmed) Minneapolis St Paul Intl (MSP) Terminal:TERMINAL 1 - LINDBERGH Duration: 3 hours, 51 minutes Nonstop Arrival: 07:31 PM Lindbergh Intl Arpt (SAN) Terminal:2 Confirmation: HCYHRT Status: Confirmed Additional Details Aircraft: Boeing 757-200 Distance: 1529 miles E-Ticket
- 7. [AS TRAVEL GRANTAPPLICATION- CHRISTIAN JAQUESHISSOM] February8, 2014 Cabin: Economy (X) Meal: Food for purchase Total Estimated Cost Air Airfare quoted amount: $557.91 USD Taxes and fees: $85.84 USD Total Estimated Cost: $643.75 USD Restrictions Quote: NONREF/PENALTY/APPLIES TICKET NOT YET ISSUED. AIRFARE QUOTED IN ITINERARY IS NOT GUARANTEED UNTIL TICKETS ARE ISSUED.