Bacteria Isolation and Characterization Presentation
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This document summarizes the results of isolating and identifying bacteria from soil samples collected in Puerto Rico. Four soil samples were collected from different locations and dates. Gram staining, PCR electrophoresis, and antibiotic resistance testing were performed on the bacteria isolated from each sample. The results showed that two isolates were gram-positive bacilli and one was a gram-negative coccus. PCR identified one isolate as positive while antibiotic resistance testing showed varying levels of resistance to different antibiotics among the isolates.
Bacteria Isolation and Characterization Presentation
- 1. ISOLATING BACTERIA FROM TROPICAL CONTAMINATED SOILS IN PUERTO RICO RISE SPRING 2015 Sara Diaz Patricia Rodriguez Camille Ruiz Justin Cotto
- 2. SAMPLING LOCATIONS Sample Coordinates Environmental description Location Date/Time Ruiz C - #1 18°6’55.1”N; 66°9’36.2”W Moist (underneath grass), 5 cm; 72°F Cayey, PR January/28/15 7:30 p.m. Cotto J - #2 18.15º N 66.18ºW Moist environment (above grass), 76ºF Cidra, PR January/29/15 8:00 a.m.
- 3. SAMPLING LOCATIONS Sample Coordinates Environmental description Location Date/Time Diaz S -#1 18°15'15.7"N 66°06'06.9"W Moist environment 80°F Aguas Buenas, P.R. January/27/1 5 4:26 p.m. Rodríguez P -#2 18°15'15.7"N 66°06'06.9"W Moist environment 80°F Aguas Buenas, P.R. January/27/1 5 4:26 p.m.
- 4. RESULTS FROM DIFFERENT PROCEDURES Process CLRV30TSA01A CLRV30M01B JCR30TSA01A Gram Stain Negative Positive Positive Bacterial Morphology Coccus Bacillus Bacillus PCR/Electrophoresis #1 Negative Negative Negative PCR/Electrophoresis #2 Positive Negative - Antibiotic Production Negative Negative Negative
- 5. GRAM STAIN RESULTS CLRV30TSA01A CLRV30M01B JCR30TSA01A
- 7. RESULTS FROM DIFFERENT PROCEDURES Process S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01 Gram Stain Positive Positive Bacterial Morphology Bacillus Bacillus PCR/Electrophoresis #1 Negative Negative PCR/Electrophoresis #2 Negative Negative Antibiotic Production Negative Negative
- 8. GRAM STAIN RESULTS S15UPRC-RISEPR30M01 S15UPRC-RISESCDM30T01B
- 9. ANTIBIOTIC RESISTANCETEST RESULTS Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A Kanamycin 2.0 cm 1.0 cm 2.0 cm Penicillin 0.0 cm 0.0 cm - Tetracycline 1.6 cm 2.4 cm 2.2 cm Gentamicin 1.9 cm 0.9 cm - Chloramphenicol 0.0 cm 0.0 cm 2.5 cm Bacitracin 0.0 cm 0.0 cm 1.5 cm Vancomycin 0.0 cm 0.0 cm - Streptomycin 1.6 cm 1.0 cm 1.9 cm
- 12. ANTIBIOTIC RESISTANCETEST RESULTS Antibiotic S15UPRC-RISESCDM30T01B Streptomycin 2.2 cm Chloramphenicol 2.3 cm Erythromycin 1.0 cm Kanamycin 1.6 cm Gentamicin 0.6 cm Antibiotic S15UPRC-RISEPR30M01 Rifampin 0.13 cm Kanamycin 0.16 cm Vancomycin 0.17cm Streptomycin 0.30 cm Tetracyclin 0.14 cm