Basics of uv spectroscopy
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This document provides an overview of ultraviolet spectroscopy. It defines spectroscopy as the study of interaction between matter and electromagnetic radiation. It explains that ultraviolet and visible (UV-Vis) absorption spectroscopy measures the attenuation of light passing through or reflecting from a sample. Some uses of UV spectroscopy mentioned are detection of functional groups, qualitative and quantitative analysis, and showing relationships between compounds. It also defines ultraviolet radiation, noting it comes primarily from the sun and has wavelengths shorter than visible light. The principle of UV spectroscopy is explained as molecules absorbing UV radiation to cause electron transitions between energy levels.
Basics of uv spectroscopy
- 2. Presentation Basics Of Ultravoilet Spectroscopy
- 3. Presented By: Rahat Inayat Ali 14CH23
- 4. Contents * Spectroscopy * Ultravoilet Spectroscopy * Ultravoilet Radiation * Principle of UV spectroscopy
- 5. Spectroscopy • Spectroscopy is the study of the interaction between matter and electromagnetic radiation. Historically, spectroscopy originated through the study of visible light dispersed according to its wavelength, by a prism.
- 6. Ultravoilet Spectroscopy • Ultravoilet and Visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a simple or after reflection from a simple surface.Absorption measurement can be at a single wavelength over an extended spectral range.
- 7. Why we use UV Spectroscopy? • Detection of functional groups • Detection of Impurities • Qualitative Analysis • Quantitative Analysis • Single Compound Without Chromophore • Drugs with chromophoric reagent • It is helps to show the relationship between different group, it is useful to detect the conjugation of the compounds.
- 8. Ultravoilet Radiation • Ultraviolet (UV) light is an electromagnetic radiation with a wavelength from 400 nm to 10 nm, shorter than that of visible light but longer than X-rays. • Where does it come from? • The sun is a major source of ultraviolet rays. Though the sun emits all of the different kinds of electromagnetic radiation, 99% of its rays are in the form of visible light, ultraviolet rays, and infrared rays (also known as heat). Man- made lamps can also emit UV radiation, and are often used for experimental purposes.
- 10. Principle of UV spectroscopy • Ultravoilet absorption spectra arise from transition of electron with in a molecule from a lower level to higher level. • A molecule absorb UV radiation of frequency, the electron in that molecule undergo transition from a lower level to higher level. • The energy can be calculated by equation. » E=hv erg
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