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sodium-potassium pump](https://image.slidesharecdn.com/ch-250223010502-fa6e7f5c/85/Ch-05-pptx-for-school-downloaded-on-structure-and-function-of-cells-15-320.jpg)
Ch.05.pptx for school downloaded on structure and function of cells
- 1. Chapter 5 Structure and Function of Plasma Membranes
- 2. You should be able to: 1. Describe the evidence that revealed the structure of the membrane. 2. Explain: selective permeability, amphipathic molecules, aquaporins, diffusion, fluid mosaic model, freeze-fracture, membrane fluidity, peripheral and integral membrane proteins, glycolipids, glycoproteins, concentration gradient, channel and carrier proteins, osmosis, facilitated diffusion, and active transport, osmoregulation, hypertonic, hypotonic, and isotonic solutions, plasmolysis, ion and gated channels, cotransport, exocytosis, endocytosis. Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
- 3. What is the structure of the membrane? 1915-membranes are lipids + proteins Where are the proteins? 1674-cells have membranes
- 4. Fig. 7-5 Lateral movement (~107 times per second) Flip-flop (~ once per month) (a) Movement of phospholipids (b) Membrane fluidity Fluid Viscous Unsaturated hydrocarbon tails with kinks Saturated hydro- carbon tails (c) Cholesterol within the animal cell membrane Cholesterol
- 5. Fig. 7-2 Hydrophilic head WATER RESULTS Membrane proteins Mouse cell Human cell Hybrid cell Mixed proteins after 1 hour N-terminus C-terminus Helix CYTOPLASMIC SIDE EXTRACELLULAR SIDE
- 6. Fig. 7-4 FREEZE-FRACTURE TECHNIQUE Extracellular layer Knife Proteins Inside of extracellular layer RESULTS Inside of cytoplasmic layer Cytoplasmic layer Plasma membrane Where are the proteins?
- 7. Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral protein CYTOPLASMIC SIDE OF MEMBRANE Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate fluid mosaic model - a membrane is a fluid lipid bilayer with a “mosaic” of various proteins embedded in it
- 8. ER 1 Transmembrane glycoproteins Secretory protein Glycolipid 2 Golgi apparatus Vesicle 3 4 Secreted protein Transmembrane glycoprotein Plasma membrane: Cytoplasmic face Extracellular face Membrane glycolipid Birth of a membrane Do membranes have sides?
- 9. (a) Transport ATP (b) Enzymatic activity Enzymes (c) Signal transduction Signal transduction Signaling molecule Receptor (d) Cell-cell recognition Glyco- protein (e) Intercellular joining (f) Attachment Major functions of membrane proteins
- 10. Transport • Osmosis, aquaporins (3e9/sec), concentration gradient Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Which way does the water go?
- 11. Hypotonic solution (a) Animal cell (b) Plant cell H2O Lysed H2O Turgid (normal) H2O H2O H2O H2O Normal Isotonic solution Flaccid H2O H2O Shriveled Plasmolyzed Hypertonic solution Which way does the water go?
- 12. Environment: 0.01 M sucrose 0.01 M glucose 0.01 M fructose “Cell” 0.03 M sucrose 0.02 M glucose Which way does the water go?
- 13. Filling vacuole 50 µm Contracting vacuole
- 14. Fig. 7-17 Passive transport Diffusion Facilitated diffusion Active transport ATP Channel protein Carrier protein
- 15. 2 EXTRACELLULAR FLUID [Na+ ] high [K+ ] low [Na+ ] low [K+ ] high Na+ Na+ Na+ Na+ Na+ Na+ CYTOPLASM ATP ADP P Na+ Na+ Na+ P 3 K+ K+ 6 K+ K+ 5 4 K+ K+ P P 1 sodium-potassium pump
- 17. Proton pump – – – – – – + + + + + + ATP H+ H+ H+ H+ H+ H+ H+ H+ Diffusion of H+ Sucrose-H+ cotransporter Sucrose Sucrose Cotransport Is the sodium-potassium pump a cotransporter?
- 18. PHAGOCYTOSIS EXTRACELLULAR FLUID CYTOPLASM Pseudopodium “Food”or other particle Food vacuole 1 µm Pseudopodium of amoeba Bacterium Food vacuole PINOCYTOSIS Plasma membrane Vesicle 0.5 µm Pinocytosis vesicles forming (arrows) in a cell lining a small blood vessel (TEM)
- 19. Fig. 7-20c RECEPTOR-MEDIATED ENDOCYTOSIS Receptor Coat protein Coated pit Ligand Coat protein Plasma membrane 0.25 µm Coated vesicle A coated pit and a coated vesicle formed during receptor- mediated endocytosis (TEMs)
- 20. You should now be able to: Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings 1. Describe the evidence that revealed the structure of the membrane. 2. Explain: selective permeability, amphipathic molecules, aquaporins, diffusion, fluid mosaic model, freeze-fracture, membrane fluidity, peripheral and integral membrane proteins, glycolipids, glycoproteins, concentration gradient, channel and carrier proteins, osmosis, facilitated diffusion, and active transport, osmoregulation, hypertonic, hypotonic, and isotonic solutions, plasmolysis, ion and gated channels, cotransport, exocytosis, endocytosis.
Editor's Notes
- #4: Figure 7.5 The fluidity of membranes
- #5: Figure 7.2 Phospholipid bilayer (cross section)
- #6: Figure 7.4 Freeze-fracture
- #8: Figure 7.10 Synthesis of membrane components and their orientation on the resulting membrane
- #9: Figure 7.9 Some functions of membrane proteins
- #11: Figure 7.13 The water balance of living cells
- #13: Figure 7.14 The contractile vacuole of Paramecium: an evolutionary adaptation for osmoregulation
- #14: Figure 7.17 Review: passive and active transport
- #15: Figure 7.16, 1–6 The sodium-potassium pump: a specific case of active transport
- #16: Figure 7.18 An electrogenic pump
- #17: Figure 7.19 Cotransport: active transport driven by a concentration gradient
- #18: Figure 7.20 Endocytosis in animal cells
- #19: Figure 7.20 Endocytosis in animal cells—receptor-mediated endocytosis