Ch 21_lecture_presentation
- 1. PowerPoint® Lecture Presentations prepared by Bradley W. Christian, McLennan Community College C H A P T E R © 2016 Pearson Education, Inc. Microbial Diseases of the Skin and Eyes 21
- 2. © 2016 Pearson Education, Inc.
- 3. © 2016 Pearson Education, Inc. Structure and Function of the Skin Learning Objective 21-1 Describe the structure of the skin and mucous membranes and the ways pathogens can invade the skin.
- 4. © 2016 Pearson Education, Inc. Structure and Function of the Skin • Epidermis: thin outer portion of skin; composed of layers of epithelial cells • Keratin: waterproofing protein coating outer layer of epidermis • Dermis: inner, thick portion of skin; composed mainly of connective tissue
- 5. © 2016 Pearson Education, Inc. Hair follicle Oil gland (produces sebum) Hair erector muscle Hair shaft Stratum corneum Sweat pore Epidermis Dermis Subcutaneous layer Duct of sweat gland Sweat gland (produces perspiration) Blood vessels NerveAdipose tissue (fat) Figure 21.1 The structure of human skin.
- 6. © 2016 Pearson Education, Inc. Structure and Function of the Skin • Perspiration provides moisture and nutrients for growth • Contains salt that inhibits microorganisms • Lysozyme breaks down bacterial cell walls • Antimicrobial peptides • Sebum secreted by oil glands contains fatty acids that inhibit pathogens
- 7. © 2016 Pearson Education, Inc. Mucous Membranes • Line the body cavities open to the exterior • Tightly packed epithelial cells attached to an extracellular matrix • Cells secrete mucus • Some cells have cilia • Often acidic • Membrane of eyes washed by tears containing lysozyme • Often folded to maximize surface area
- 8. © 2016 Pearson Education, Inc. Check Your Understanding Moisture in perspiration encourages microbial growth. What perspiration factors discourage growth? 21-1
- 9. © 2016 Pearson Education, Inc. Normal Microbiota of the Skin Learning Objective 21-2 Provide examples of normal skin microbiota, and state the general locations and ecological roles of its members.
- 10. © 2016 Pearson Education, Inc. Normal Microbiota of the Skin • Resistant to drying and high salt concentration • Large numbers of gram-positive cocci • Staphylococci • Micrococci • Areas with moisture have higher populations • Metabolize sweat and contribute to body odor
- 11. © 2016 Pearson Education, Inc. Normal Microbiota of the Skin • Gram-positive pleomorphic rods (diphtheroids) • Propionibacterium acnes inhabits hair follicles; anaerobic • Produce acids that maintain low skin pH • Corynebacterium xerosis occupy the skin surface; aerobic • Yeast • Malassezia furfur; causes dandruff
- 12. © 2016 Pearson Education, Inc. Check Your Understanding Are skin bacteria more likely to be gram-positive or gram-negative? 21-2
- 13. © 2016 Pearson Education, Inc. Microbial Diseases of the Skin Learning Objectives 21-3 Differentiate staphylococci from streptococci, and name several skin infections caused by each. 21-4 List the causative agent, mode of transmission, and clinical symptoms of Pseudomonas dermatitis, otitis externa, acne, and Buruli ulcer.
- 14. © 2016 Pearson Education, Inc. Microbial Diseases of the Skin Learning Objective 21-5 List the causative agents, modes of transmission, and symptoms of warts, smallpox, monkeypox, chickenpox, shingles, cold sores, measles, rubella, fifth disease, hand-foot-mouth disease, and roseola.
- 15. © 2016 Pearson Education, Inc. Microbial Diseases of the Skin Learning Objectives 21-6 Differentiate cutaneous from subcutaneous mycoses and provide an example of each. 21-7 List the causative agent and predisposing factors for candidiasis. 21-8 List the causative agent, mode of transmission, clinical symptoms, and treatment for scabies and pediculosis.
- 16. © 2016 Pearson Education, Inc. Microbial Diseases of the Skin • Vesicles: small, fluid-filled lesions • Bullae: vesicles larger than 1 cm in diameter • Macules: flat, reddened lesions • Papules: raised lesions • Pustules: raised lesions with pus • Exanthem: skin rash arising from a disease • Enanthem: rash on mucous membranes arising from a disease
- 17. © 2016 Pearson Education, Inc. Figure 21.2 Skin lesions. Epidermis 1 cm Fluid Dermis Vesicle Bulla 1 cm Fluid 1 cm 1 cm Pus Macule Pustule (papule)
- 18. © 2016 Pearson Education, Inc. Staphylococcal Skin Infections • Staphylococci • Spherical gram-positive bacteria; form irregular clusters • Many produce coagulase • Enzyme that clots fibrin in the blood • Used to identify types of staphylococci
- 19. © 2016 Pearson Education, Inc. Clinical Focus 21.1b
- 20. © 2016 Pearson Education, Inc. Staphylococcal Skin Infections • Staphylococcus epidermidis • Ninety percent of normal skin microbiota • Healthcare-associated pathogen • Produces biofilm on catheters • Coagulase-negative
- 21. © 2016 Pearson Education, Inc. Figure 21.3 Coagulase-negative staphylococci. Biofilm Surface of catheter Biofilm Catheter sur- face with adhering bacteria. Biofilm, light green, is beginning to appear. Most of the bacteria produc- ing the slime are not visible under the biofilm.
- 22. © 2016 Pearson Education, Inc. Staphylococcal Skin Infections • Staphylococcus aureus • Carried in the nasal passages of 20% of the population • Golden-yellow colonies • Coagulase-positive • May produce damaging toxins and cause sepsis • Avoids host defenses in the skin • Secretes proteins and toxins that kill phagocytic cells • MRSA strains are antibiotic-resistant
- 23. © 2016 Pearson Education, Inc. Clinical Focus 21.1c
- 24. © 2016 Pearson Education, Inc. Staphylococcal Skin Infections • Folliculitis: infections of the hair follicles • Sty: folliculitis of an eyelash • Furuncle (boil): a type of abscess; localized region of pus surrounded by inflamed tissue • Carbuncle: damage and inflammation of deep tissue from a spreading furuncle • Impetigo: crusting (nonbullous) sores, spread by autoinoculation
- 25. © 2016 Pearson Education, Inc. Figure 21.4 Lesions of impetigo.
- 26. © 2016 Pearson Education, Inc. Staphylococcal Skin Infections • Scalded skin syndrome • Bullous impetigo • Toxin B causes exfoliation • Pemphigus neonatorum: impetigo of the newborn • Toxic shock syndrome (TSS) • Fever, vomiting, shock, and organ failure caused by toxic shock syndrome toxin 1 (TSST-1) in the bloodstream
- 27. © 2016 Pearson Education, Inc. Figure 21.5 Lesions of scalded skin syndrome.
- 28. © 2016 Pearson Education, Inc. Diseases in Focus: Macular Rashes • A 4-year-old boy with a history of cough, conjunctivitis, and fever (38.3°C) now has a macular rash that started on his face and neck and is spreading to the rest of his body. • Can you identify infections that could cause these symptoms?
- 29. © 2016 Pearson Education, Inc. Diseases in Focus 21.1 (1 of 2)
- 30. © 2016 Pearson Education, Inc. Diseases in Focus 21.1 (2 of 2)
- 31. © 2016 Pearson Education, Inc. Streptococcal Skin Infections • Gram-positive cocci in chains • Produce hemolysins that lyse red blood cells • Beta-hemolytic streptococci often cause disease • Streptococci differentiated into groups A through T based on antigenic cell wall carbohydrates
- 32. © 2016 Pearson Education, Inc. Streptococcal Skin Infections • Group A streptococci (GAS), also known as Streptococcus pyogenes • Eighty immunological types • Produce virulence factors • Streptolysins: lyse RBCs • M proteins: external to the cell wall; allow adherence and immune system avoidance • Hyaluronidase: dissolves connective tissue • Streptokinases: dissolve blood clots
- 33. © 2016 Pearson Education, Inc. Figure 21.6 The M protein of group A beta-hemolytic streptococci. M protein on fibrils Fibrils lacking M protein
- 34. © 2016 Pearson Education, Inc. Streptococcal Skin Infections • Erysipelas • S. pyogenes infects the dermal layer of the skin • Causes local tissue destruction and sepsis • Necrotizing fasciitis • "Flesh-eating" disease • Extotoxin A produced by S. pyogenes acts as a superantigen • Streptococcal toxic shock syndrome • Similar to staphylococcal TSS
- 35. © 2016 Pearson Education, Inc. Figure 21.7 Lesions of erysipelas, caused by group A betahemolytic streptococcal toxins.
- 36. © 2016 Pearson Education, Inc. Figure 21.8 Necrotizing fasciitis due to group A streptococci.
- 37. © 2016 Pearson Education, Inc. Infections by Pseudomonads • Pseudomonas aeruginosa • Gram-negative, aerobic rod • Pyocyanin produces a blue-green pus • Produces exo- and endotoxins; grows in biofilms • Pseudomonas dermatitis • Self-limiting rash acquired in swimming pools • Otitis externa • "Swimmer's ear" • Opportunistic in burn patients • Resistant to many antibiotics
- 38. © 2016 Pearson Education, Inc. Buruli Ulcer • Caused by Mycobacterium ulcerans • Produces the toxin mycolactone • Enters via a break in the skin or an insect bite • Causes deep, damaging ulcers • May require amputation • Exceeds incidence of leprosy • Primarily found in western and central Africa
- 39. © 2016 Pearson Education, Inc. Acne • Most common skin disease in humans • Skin cells shed in the hair follicles and combine with sebum • Causes blockages • Sebum formation is affected by hormones, not diet • Comedonal (mild) acne • Easily treated with topical formations
- 40. © 2016 Pearson Education, Inc. Acne • Inflammatory (moderate) acne • Caused by Propionibacterium acnes • Metabolizes sebum; fatty acids produce an inflammatory response • Treated with antibiotics and benzoyl peroxide • Nodular cystic (severe) acne • Inflamed lesions with pus deep in the skin
- 41. © 2016 Pearson Education, Inc. Figure 21.9 Severe acne.
- 42. © 2016 Pearson Education, Inc. Check Your Understanding Which bacterial species features the virulence factor M protein? 21-3 What is the common name for otitis externa? 21-4
- 43. © 2016 Pearson Education, Inc. Viral Diseases of the Skin • Many are transmitted via respiratory routes and are systemic • Many cause problems in children and developing fetuses
- 44. © 2016 Pearson Education, Inc. Warts • Papillomas: small skin growths • Transmitted via contact • Caused by papillomavirus • More than 50 types • Some cause skin and cervical cancers • Treated with cryotherapy, electrodesiccation, or salicylic acid
- 45. © 2016 Pearson Education, Inc. Smallpox (Variola) • Caused by an orthropoxvirus • Two forms of the disease • Variola major has 20% mortality • Variola minor has <1% mortality • Transmitted via the respiratory route, moves into the bloodstream, and infects the skin • Completely eradicated from the human population by vaccination • Potential bioterrorism agent
- 46. © 2016 Pearson Education, Inc. Smallpox (Variola) • Monkeypox • Related to smallpox • Endemic to small animals in Africa • Jumps from animals to humans • Mortality of 1–10% • Prevention by the smallpox vaccination
- 47. © 2016 Pearson Education, Inc. Figure 21.10 Smallpox lesions.
- 48. © 2016 Pearson Education, Inc. Chickenpox (Varicella) and Shingles (Herpes Zoster) • Chickenpox (varicella) • Herpesvirus varicella-zoster (human herpesvirus 3) • Transmitted via the respiratory route • Causes pus-filled vesicles • Reye's syndrome: severe complications of chickenpox; vomiting and brain dysfunction • Aspirin use increases risk • Virus becomes latent in the central nerve ganglia • Prevented by a live attenuated vaccine • Breakthrough varicella can occur if previously vaccinated
- 49. © 2016 Pearson Education, Inc. Figure 21.11a Chickenpox (varicella) and shingles (herpes zoster). Nerve cell in dorsal root ganglion Latent viral DNA Viruses move up peripheral nerve Spinal cord Initial infection: chickenpox (varicella)
- 50. © 2016 Pearson Education, Inc. Chickenpox (Varicella) and Shingles (Herpes Zoster) • Shingles (herpes zoster) • Reactivation of the latent varicella-zoster virus that moves along peripheral nerves to the skin • Due to stress or lowered immunity • Follows the distribution of affected cutaneous sensory nerves • Limited to one side of the body • Postherpetic neuralgia • Prevention via the zoster vaccine • Antiviral drugs may lessen symptoms
- 51. © 2016 Pearson Education, Inc. Figure 21.11b Chickenpox (varicella) and shingles (herpes zoster). Activation of viral DNA in nerve cell Viruses Viruses move down peripheral nerve Spinal cord Recurrence of infection: shingles (herpes zoster)
- 52. © 2016 Pearson Education, Inc. Herpes Simplex • Human herpesvirus 1 (HSV-1) and 2 (HSV-2) • HSV-1 is spread primarily by oral or respiratory routes • HSV-2 is spread primarily sexually • Ninety percent of the population is infected • Usually develop as cold sores or fever blisters • Not the cause of canker sores
- 53. © 2016 Pearson Education, Inc. Figure 21.12 Cold sores, or fever blisters, caused by herpes simplex virus.
- 54. © 2016 Pearson Education, Inc. Herpes Simplex • HSV-1 remains latent in trigeminal nerve ganglia • Outbreaks are triggered by the sun, stress, or hormonal changes • HSV-2 remains latent in sacral nerve ganglia near the spine • Herpes gladiatorum: vesicles on the skin • Herpetic whitlow: vesicles on the fingers • Herpes encephalitis: virus spreads to the brain • Treated with acyclovir
- 55. © 2016 Pearson Education, Inc. Figure 21.13 Site of latency of herpes simplex type 1 in the trigeminal nerve ganglion. Trigeminal nerve Ganglion Site of viral latency Site of active lesion
- 56. © 2016 Pearson Education, Inc. Measles (Rubeola) • Viral disease transmitted by the respiratory route • Cold-like symptoms, macular rash • Koplik's spots • Red spots on the oral mucosa opposite the molars • Encephalitis in 1 in 1000 cases • Subacute sclerosing panencephalitis • Rare; occurs 1 to 10 years after measles recovery • Prevented by the MMR (measles, mumps, rubella) vaccine • Children under 1 year old cannot receive the vaccine
- 57. © 2016 Pearson Education, Inc. Figure 21.14 The rash of small raised spots typical of measles (rubeola).
- 58. © 2016 Pearson Education, Inc. Rubella • German measles • Rubella virus • Macular rash and light fever • Transmitted via the respiratory route; 2 to 3 week incubation • Congenital rubella syndrome • Fetal damage, deafness, heart defects, mental retardation • Fifteen percent mortality • Prevented by the MMR vaccine • Not recommended for pregnant women
- 59. © 2016 Pearson Education, Inc. Figure 21.15 The rash of red spots characteristic of rubella.
- 60. © 2016 Pearson Education, Inc. Other Viral Rashes • Fifth disease (erythema infectiosum) • Human parvovirus B19 • Mild flulike symptoms; "slapped-cheek" facial rash • Roseola • Human herpesviruses 6 and 7 • High fever; body rash; recovery within 1 to 2 days • Hand-foot-mouth disease • Enteroviruses • Spread via mucous or saliva (usually children) • Fever and sore throat; rash on the hands, feet, mouth, and tongue
- 61. © 2016 Pearson Education, Inc. Diseases in Focus: Vesicular and Pustular Rashes • An 8-year-old boy has a rash consisting of vesicular lesions of 5 days' duration on his neck and stomach. Within 5 days, 73 students in his elementary school have an illness matching the case definition for this disease. • Can you identify infections that could cause these symptoms?
- 62. © 2016 Pearson Education, Inc. Diseases in Focus 21.2 (1 of 2)
- 63. © 2016 Pearson Education, Inc. Diseases in Focus 21.2 (2 of 2)
- 64. © 2016 Pearson Education, Inc. Check Your Understanding How did the odd naming of "fifth disease" arise? 21-5
- 65. © 2016 Pearson Education, Inc. Fungal Diseases of the Skin and Nails • Mycosis: fungal infection of the body • Cutaneous mycoses • Colonize the hair, nails, and outer epidermis • Metabolize keratin • Dermatomycoses • Informally known as tineas or ringworm • Tinea capitis: scalp ringworm • Tinea cruris: jock itch • Tinea pedis: athlete's foot • Tinea unguium: ringworm of nails
- 66. © 2016 Pearson Education, Inc. Fungal Diseases of the Skin and Nails • Cutaneous mycoses (cont'd) • Genera of fungi involved: • Trichophyton • Microsporum • Epidermophyton • Treatment is usually topical drugs (miconazole and clotrimazole)
- 67. © 2016 Pearson Education, Inc. Figure 21.16 Dermatomycoses.
- 68. © 2016 Pearson Education, Inc. Subcutaneous Mycoses • More serious than cutaneous mycoses • Penetrate the stratum corneum • Usually caused by fungi that inhabit the soil • Sporotrichosis • Caused by Sporothrix schenkii; dimorphic fungus • Enters a wound; forms a small ulcer • Treated with potassium iodide
- 69. © 2016 Pearson Education, Inc. Candidiasis • Overgrowth of Candida albicans (yeast) • Forms pseudohyphae, making it resistant to phagocytosis • Occurs in the skin and mucous membranes of the genitourinary tract and mouth • Thrush: C. albicans infection of the oral cavity • Results when antibiotics suppress competing bacteria or a change occurs in the mucosal pH • Fulminating disease in the immunosuppressed
- 70. © 2016 Pearson Education, Inc. Figure 21.17 Candidiasis. Chlamydoconidia Pseudohyphae Blastoconidia Candida albicans Oral candidiasis, or thrush
- 71. © 2016 Pearson Education, Inc. Check Your Understanding How do sporotrichosis and tineas differ? How are they similar? 21-6 How might penicillin use result in candidiasis? 21-7
- 72. © 2016 Pearson Education, Inc. Scabies • Caused by Sarcoptes scabiei mites • Burrow in the skin to lay eggs • Causes inflammatory skin lesions • Transmitted via intimate contact • Treatment with permethrin
- 73. © 2016 Pearson Education, Inc. Figure 21.18 Scabies mites in skin. Mites
- 74. © 2016 Pearson Education, Inc. Pediculosis (Lice) • Pediculus humanus capitis (head louse) • P. h. corporis (body louse) • Feed on blood from the host • Lay eggs (nits) on the hair and attach to the shafts • Treatment with topical insecticides (permethrin or pyrethrin) • Malathion, lindane, or ivermectin are used in cases of resistance
- 75. © 2016 Pearson Education, Inc. Figure 21.19 Louse and louse egg case.
- 76. © 2016 Pearson Education, Inc. Diseases in Focus: Patchy Redness and Pimplelike Conditions • An 11-month-old boy comes to a clinic with a 1-week history of an itchy red rash under his arms. The rash seems to bother him more at night and he has no fever. • Can you identify infections that could cause these symptoms?
- 77. © 2016 Pearson Education, Inc. Diseases in Focus 21.3 (1 of 3)
- 78. © 2016 Pearson Education, Inc. Diseases in Focus 21.3 (2 of 3)
- 79. © 2016 Pearson Education, Inc. Diseases in Focus 21.3 (3 of 3)
- 80. © 2016 Pearson Education, Inc. Check Your Understanding What diseases, if any, are spread by head lice, such as Pediculus humanus capitis? 21-8
- 81. © 2016 Pearson Education, Inc. Microbial Diseases of the Eye Learning Objectives 21-9 Define conjunctivitis. 21-10 List the causative agent, mode of transmission, and clinical symptoms of these eye infections: ophthalmia neonatorum, inclusion conjunctivitis, trachoma. 21-11 List the causative agent, mode of transmission, and clinical symptoms of these eye infections: herpetic keratitis, Acanthamoeba keratitis.
- 82. © 2016 Pearson Education, Inc. Inflammation of the Eye Membranes: Conjunctivitis • An inflammation of the conjunctiva • Also called red eye or pinkeye • Commonly caused by Haemophilus influenzae • Also caused by adenoviruses • Can be caused by pseudomonads associated with unsanitary contact lenses
- 83. © 2016 Pearson Education, Inc. Big Picture: Fungal Keratitis • Fungal keratitis • Outbreak in 2005–2006 was due to contaminated contact lens solution • Caused by Fusarium spp. filamentous fungi • Form biofilms that form a mat of hyphae on contact lenses • Resistant to many antifungal drugs • Natamycin: only antifungal approved to treat fungal keratitis
- 84. © 2016 Pearson Education, Inc. Big Picture: Fungal Keratitis
- 85. © 2016 Pearson Education, Inc. Ophthalmia • Caused by Neisseria gonorrhoeae • Large amount of pus forms; ulceration of corneas results • Untreated cases may lead to blindness • Transmitted to a newborn's eyes during passage through the birth canal • Prevented by treating a newborn's eyes with antibiotics
- 86. © 2016 Pearson Education, Inc. Inclusion Conjunctivitis • Caused by Chlamydia trachomatis • Bacterium that grows as an obligate intracellular parasite • Transmitted to a newborn's eyes during passage through the birth canal • Spread through swimming pool water • Treated with tetracycline
- 87. © 2016 Pearson Education, Inc. Trachoma • Caused by some serotypes of Chlamydia trachomatis • Leading cause of blindness worldwide • Transmitted via hand contact or flies • Infection causes permanent scarring; scars abrade the cornea, leading to blindness • Secondary infections can also be a factor • Oral azithromycin are used in treatment
- 88. © 2016 Pearson Education, Inc. Figure 21.20 Trachoma.
- 89. © 2016 Pearson Education, Inc. Check Your Understanding What is the common name of inclusion conjunctivitis? 21-9 Why have antibiotics almost entirely replacedthe less expensive use of silver nitrate for preventing ophthalmia neonatorum? 21-10
- 90. © 2016 Pearson Education, Inc. Other Infectious Diseases of the Eye • Keratitis • Inflammation of the cornea • Bacterial (United States) • Fusarium and Aspergillus (Africa and Asia) • Herpetic keratitis • Caused by herpes simplex virus 1 (HSV-1) • Infects cornea and may cause blindness • Treated with trifluridine
- 91. © 2016 Pearson Education, Inc. Other Infectious Diseases of the Eye • Acanthamoeba keratitis • Ameba transmitted via water and soil • Associated with unsanitary contact lenses • Mild inflammation followed by severe pain • Treatment with propamidine isethionate and neomycin • May require a corneal transplant
- 92. © 2016 Pearson Education, Inc. Diseases in Focus: Microbial Diseases of the Eye • In the morning a 20-year-old man has eye redness with a crust of mucus. The condition resolves with topical antibiotic treatment. • Can you identify infections that could cause these symptoms?
- 93. © 2016 Pearson Education, Inc. Diseases in Focus 21.4 (1 of 2)
- 94. © 2016 Pearson Education, Inc. Diseases in Focus 21.4 (2 of 2)
- 95. © 2016 Pearson Education, Inc. Check Your Understanding Of the two eye diseases herpetic keratitis and Acanthamoeba keratitis, which is the more likely to be caused by an organism actively reproducing in saline solutions for contact lenses? 21-11