N=1 animal showed some questionable cystic changes in the hilar region that may have been due to tissue and sectioning issues rather than pathology. The peripheral region also had questionable cystic areas but similar areas were seen in control animals. Ductal plate formation was reduced or absent in the hilar region of an E16.5 double knockout animal. Peripherally, ductal plates were difficult to identify and there may have been increased background staining of cells rather than disorganized CK19-positive cells. Further study is needed to identify upregulated pathways leading to changes seen at P60 and P30 if not due to proliferation.
