Codons Optimization by Creative Biogene
- 1. Codon Optimization Technology Tel: 1-631-626-9181 Fax: 1-631-614-7828 Email: info@creative-biogene.com Website: http://www.creative-biogene.com
- 2. Codon optimization is one of the key steps in achieving the high-level expression of target gene. There are some key factors for consideration including transcription efficiency, translation efficiency, gene synthesis and protein folding. Creative Biogene can optimize almost perfectly these key factors in codon optimization.
- 3. Some necessary consideration factors in codon optimization
- 4. mRNA C D
- 5. tRNA abundance Owing to different tRNAs identify several synonymous codons and the content of these synonymous tRNAs are different, therefore, the efficiency of translation also depends on the number of tRNA. Preferred codons are those that can base pair optimally with the most abundant tRNA. Generally this involves Watson - Crick pairing or, when bases are modified in the tRNA, some modifications in optimal binding occur. Codon usage bias There are obvious biases of synonymous codon in bacteria, Escherichia coli, yeast and some expression systems of higher biological. This can directly affect the efficiency of translation. So when one gene is expressed in a heterologous system, the codon usage bias should be taken into account.
- 6. Complete mRNA structure Next to 5'UTR and 3'UTR, The complete mRNA structure also has significant effect on the process of protein translation. Recently, from the systematic study of major factors which affect the protein expression, we know that the folding of mRNA, especially the mRNA structure near the RBS, plays an important role in protein expression. For example, the level of protein expression can be greatly improved by reducing the secondary structure near the start codon.
- 7. The theory of gene design Codon adaptation index (CAI) CAI measures the proximity between the codon which is used by one gene and the codon which is used in gene with high expression. CAI is between 0 and 1. The closer that the CAI is to 1, the higher level of gene expression is. In general, CAI>0.8 means that gene expression has a high level. Codon correlation index (CCI) Researches show that codon correlation of specific host exert considerable influence on the expression of heterologous genes. Codon correlation, such as codon usage, double codon usage, dynamic of translation phase and GC content, describe an integrated evolutionary environment characteristic of species codon selection.
- 9. Creative Biogene offers gene synthesis and cloning services for your scientific research as follows: Codon optimization for any given expression system (upon request); Synthesis genes of interest with or without tags; Cloning the target sequence into a standard vector or a vector of your choice; Sequencing the final construct to confirm 100% matching your requested sequence; Delivery of purified plasmid DNA construct and its bacterial stock.
- 10. Thank You! Tel: 1-631-626-9181 Fax: 1-631-614-7828 Email: info@creative-biogene.com Website: http://www.creative-biogene.com