During the first 2-3 weeks of conception, the embryo develops three primary germ layers - ectoderm, mesoderm, and endoderm - which give rise to the skin, muscles, and digestive system respectively. The heart begins beating by the end of the third week. By 4 weeks, the neural tube has closed to form the brain and spinal cord, and the heart begins regularly circulating blood through the main vessels. By 5 weeks, the brain has differentiated into 5 areas and 10 pairs of cranial nerves are present. Maternal changes during this time include breast tenderness, frequent urination, nausea and vomiting, and fatigue.