CORTECS Columns Infographic
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The document provides information on Waters CORTECS UPLC and HPLC columns including ordering details and specifications. It lists the available chemistries (C18, C18+, etc.), dimensions (2.1mm x 50mm, etc.), and part numbers. The document explains that CORTECS columns are available with 1.6um and 2.7um particles to enable methods transfer between UPLC, UHPLC, and HPLC systems for improved speed, resolution, and solvent usage. A total of 25 column configurations are available across the different chemistries and dimensions.
![www.waters.com/cortecs
CORTECS ORDERING INFORMATION
Frequently ordered CORTECS UPLC 1.6 µm Columns for UPLC Instruments
C18 C18+ T3 Shield RP18 C8 Phenyl HILIC
2.1 x 50 mm 186007093 186007114 186008497 186008692 186008399 186008379 186007104
2.1 x 100 mm 186007095 186007116 186008499 186008694 186008401 186008381 186007106
2.1 x 150 mm 186007096 186007117 186008500 186008695 186008402 186008382 186007107
Frequently ordered CORTECS 2.7 µm Columns for UHPLC Instruments
C18 C18+ T3 Shield RP18 C8 Phenyl HILIC
3.0 x 50 mm 186007370 186007400 186008487 186008672 186008349 186008329 186007385
3.0 x 100 mm 186007372 186007402 186008489 186008674 186008351 186008331 186007387
3.0 x 150 mm 186007373 186007403 186008490 186008675 186008352 186008332 186007388
Frequently ordered CORTECS 2.7 µm Columns for HPLC Instruments
C18 C18+ T3 Shield RP18 C8 Phenyl HILIC
4.6 x 50 mm 186007375 186007405 186008492 186008682 186008369 186008339 186007390
4.6 x 100 mm 186007377 186007407 186008494 186008684 186008371 186008341 186007392
4.6 x 150 mm 186007378 186007408 186008495 186008685 186008372 186008342 186007393
For a complete list of all CORTECS Column configurations visit:
©2017 Waters Corporation. Waters, The Science of What’s Possible, Alliance, ACQUITY, UPLC,
and CORTECS are registered trademarks of Waters Corporation. June 2017 720005984EN LM-SIG
[ CORTECS COLUMNS ]
www.waters.com/cortecs
CORTECS
PARTICLE SIZE
Equivalent Porous Particles
Efficiency Backpressure
1.6 µm
2.7 µm
1.3 µm
2.2 µm
1.8 µm
3.1 µm
CONSISTENT, REPRODUCIBLE PERFORMANCE,
AND LONG COLUMN LIFETIMES
O Si
O
O
O Si
O
O
HILICC8C18C18+
O Si
O
O
Phenyl
O Si
O
O
T3
O Si
O
O
Shield RP18
O Si
CH3
CH3
Polar Group
BENEFITS
¡ Unique column selectivity
with industry-leading
reproducibility
¡ Exceptional peak shape
and loading capacity
without the need for
ion-pair reagents
¡ Improved signal-to-noise
performance in LC and
LC-MS applications.
A traditional
C18-bonded
phase which exhibits
balanced retention
of acids, bases, and
neutrals at low-
and mid-range pH.
BENEFITS
¡ Superb resolution
and retention for
complex mixtures.
¡ Stability and ability to
retain a variety of
analytes (most popular
choice in method
development)
BENEFITS
¡ The larger 120Å pore
diameter, and lower C18
ligand density, are both
designed to increase
polar analyte retention
while maintaining
excellent non-polar
retention.
¡ The increased pore
diameter allow the
use of 100% aqueous
mobile phases, which
is desirable in retaining
polar analytes.
An excellent choice
for separating strong
hydrophobic analytes
that are very well
retained on a
C18 column.
BENEFITS
¡ Less hydrophobic than
traditional C18 columns.
¡ Highly reproducible
ligand bonding.
BENEFITS
¡ The phenyl ring provides
Pi-Pi bond interactions
that can be enhanced by
using methanol instead
of acetonitrile as the
organic modifier.
BENEFITS
¡ Gives unique selectivity
primarily for phenolic
compounds when
compared to typical
C18 columns.
¡ Provides an aqueous
shield over undesired
acidic silanol groups on
the particles surface.
¡ Inhibits secondary
interactions between
basic analytes and
these acidic silanols,
with results in better
peak shape.
BENEFITS
¡ HILIC uses mobile
phases with a high
concentration of organic
solvent which enables
effective desolvation of
analytes in the MS
source, resulting in
improved MS response
and sensitivity.
Designed to give
balanced retention
for both polar and
non-polar molecules
when operating
under reversed
phase LC conditions.
A general purpose,
high-efficiency,
reversed-phase
column which
features a
positively charged
surface modification.
An excellent method
development column,
as it gives unique
selectivity primarily for
compounds that have
high aromaticity, when
compared to typical
C18 columns.
An excellent
method development
column as the
embedded polar
functional group
provides alternative
selectivity due to
hydrogen bonding.
Hydrophilic-interaction
chromatography
(HILIC) is a separation
mode that can be
used to improve
the retention
of extremely
polar analytes.
•Higher efficiency*
•Increased throughput
•Lower backpressure*
•Fully scalable
*Compared to fully-porous particles.
CORTECSSOL
ID-CORE PARTICLE
TOMAN
IPULATE SELECTV
ITY
6
CH
EMISTRI
ES
REV
ERSED PH
ASE
1
2
3
4 5
AU
0.00
0.01
0.02
AU
0.00
0.01
0.02
AU
0.00
0.01
0.02
12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0
3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0
AU
0.00
0.01
0.02
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
Minutes
1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Fully Porous C18, 5 µm
4.6 x 150 mm
1.00 mL /min
Alliance®
HPLC
CORTECS®
2.7 µm C18
4.6 x 75 mm
1.85 mL/min
Alliance HPLC
CORTECS 1.6 µm C18
2.1 x 50 mm
0.65 mL/min
ACQUITY®
UPLC®
H-Class
CORTECS 2.7 µm C18
3.0 x 75 mm
0.79 mL/min
ACQUITY Arc UHPLC
Solid-Core Particle
The tightly controlled
thickness of a highly porous
silica layer surrounding
the inner solid-core yields
reproducible retention and
method robustness for
a wide range of sample
conditions.
Particle Diameter
Monodisperse particle
sizing provides highly
permeable columns
and, consequently,
low backpressures.
Bonding Technology
Packed with solid-core
particles, CORTECS
Columns complement
our family of particle
technologies, offering
unique ligand attributes
that aid in method
development.
Packing Efficiency
The increased efficiency of a
solid-core particle produces
more chromatographic
resolution, which helps
reduce the effort to separate
co-eluting peaks.
System: ACQUITY UPLC H-Class System
with ACQUITY PDA
Column: CORTECS, 2.1 x 50 mm
Mobile Phase A: 10 mM Ammonium Formate pH 3
Mobile Phase B: Acetonitrile
Gradient: 90%A to 65%A in 3.00 min
Hold 65%A for 0.25 min
65%A to 90%A in 0.25 min
Total run time: 5 min
Flow rate: 0.46 mL/min
Injection volume: 1 µL
Column temp.: 30 °C
Detection: UV at 280 nm
Sample diluent: 10% Acetonitrile/90% Water
Compounds:
1. Norepinephrine
2. Epinephrine
3. Dopamine
4. VMA
5. MHPG
6. Seratonin
7. DOPAC
8. 5-HIAA
9. HVA
Initial method
Transfer to 2.7 µm
4X faster method
2X less solvent
Compatible with
UHPLC systems
4X less solvent
Transfer to 1.6 µm
9X faster method
15X less solvent
HPLCHPLCUHPLCUPLC
9XFASTER
4XFASTER
It is essential that robust methods be created
quickly and easily while being compatible with a
wide range of chromatographic systems. With
particle sizes ideal for HPLC, UHPLC, and UPLC
platforms, you can be assured a simple, and
seamless transfer with consistent results across
different particle sizes and column configurations.
SCALABILITY
AU
0.000
0.027
AU
0.000
0.045
AU
0.000
0.045
AU
0.000
0.036
AU
0.000
0.036
AU
0.000
0.024
Minutes
0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50
1
2
3
4
5
6
7
8
91
2
3
4
5,6 7
8
9
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6 7
8
9
1
2
3
4
5
6
7
8
9
1 2 3
4 5
6 7
8
9
CORTECS
Shield RP18
CORTECS C18+
CORTECS C18
CORTECS C8
CORTECS
Phenyl
CORTECS T3
7
CH
EMISTRI
ES
COLUMN
25
CON
FIGURATI
O
NS
COLUMN
Separation obtained across the two CORTECS particle sizes and three instrument
classes. CORTECS 2.7 µm Columns provide maximum flexibility, designed for
improved performance on HPLC and UHPLC instrumentation. CORTECS UPLC
1.6 µm Columns provide ultimate performance on ACQUITY UPLC Instrumentation.
TWO FULLY SCALABLE PARTICLE SIZES
TO MODERNIZE YOUR EXISTING METHODS
The Truth
About Solid-Core
Unleash the power
of your laboratory’s
instrumentation
Built on Waters proven solid-core technology, CORTECS 1.6 µm and 2.7 µm
Columns deliver new levels of chromatographic performance.](https://image.slidesharecdn.com/thetruthaboutsolid-core-180525131341/85/CORTECS-Columns-Infographic-1-320.jpg)
CORTECS Columns Infographic
- 1. www.waters.com/cortecs CORTECS ORDERING INFORMATION Frequently ordered CORTECS UPLC 1.6 µm Columns for UPLC Instruments C18 C18+ T3 Shield RP18 C8 Phenyl HILIC 2.1 x 50 mm 186007093 186007114 186008497 186008692 186008399 186008379 186007104 2.1 x 100 mm 186007095 186007116 186008499 186008694 186008401 186008381 186007106 2.1 x 150 mm 186007096 186007117 186008500 186008695 186008402 186008382 186007107 Frequently ordered CORTECS 2.7 µm Columns for UHPLC Instruments C18 C18+ T3 Shield RP18 C8 Phenyl HILIC 3.0 x 50 mm 186007370 186007400 186008487 186008672 186008349 186008329 186007385 3.0 x 100 mm 186007372 186007402 186008489 186008674 186008351 186008331 186007387 3.0 x 150 mm 186007373 186007403 186008490 186008675 186008352 186008332 186007388 Frequently ordered CORTECS 2.7 µm Columns for HPLC Instruments C18 C18+ T3 Shield RP18 C8 Phenyl HILIC 4.6 x 50 mm 186007375 186007405 186008492 186008682 186008369 186008339 186007390 4.6 x 100 mm 186007377 186007407 186008494 186008684 186008371 186008341 186007392 4.6 x 150 mm 186007378 186007408 186008495 186008685 186008372 186008342 186007393 For a complete list of all CORTECS Column configurations visit: ©2017 Waters Corporation. Waters, The Science of What’s Possible, Alliance, ACQUITY, UPLC, and CORTECS are registered trademarks of Waters Corporation. June 2017 720005984EN LM-SIG [ CORTECS COLUMNS ] www.waters.com/cortecs CORTECS PARTICLE SIZE Equivalent Porous Particles Efficiency Backpressure 1.6 µm 2.7 µm 1.3 µm 2.2 µm 1.8 µm 3.1 µm CONSISTENT, REPRODUCIBLE PERFORMANCE, AND LONG COLUMN LIFETIMES O Si O O O Si O O HILICC8C18C18+ O Si O O Phenyl O Si O O T3 O Si O O Shield RP18 O Si CH3 CH3 Polar Group BENEFITS ¡ Unique column selectivity with industry-leading reproducibility ¡ Exceptional peak shape and loading capacity without the need for ion-pair reagents ¡ Improved signal-to-noise performance in LC and LC-MS applications. A traditional C18-bonded phase which exhibits balanced retention of acids, bases, and neutrals at low- and mid-range pH. BENEFITS ¡ Superb resolution and retention for complex mixtures. ¡ Stability and ability to retain a variety of analytes (most popular choice in method development) BENEFITS ¡ The larger 120Å pore diameter, and lower C18 ligand density, are both designed to increase polar analyte retention while maintaining excellent non-polar retention. ¡ The increased pore diameter allow the use of 100% aqueous mobile phases, which is desirable in retaining polar analytes. An excellent choice for separating strong hydrophobic analytes that are very well retained on a C18 column. BENEFITS ¡ Less hydrophobic than traditional C18 columns. ¡ Highly reproducible ligand bonding. BENEFITS ¡ The phenyl ring provides Pi-Pi bond interactions that can be enhanced by using methanol instead of acetonitrile as the organic modifier. BENEFITS ¡ Gives unique selectivity primarily for phenolic compounds when compared to typical C18 columns. ¡ Provides an aqueous shield over undesired acidic silanol groups on the particles surface. ¡ Inhibits secondary interactions between basic analytes and these acidic silanols, with results in better peak shape. BENEFITS ¡ HILIC uses mobile phases with a high concentration of organic solvent which enables effective desolvation of analytes in the MS source, resulting in improved MS response and sensitivity. Designed to give balanced retention for both polar and non-polar molecules when operating under reversed phase LC conditions. A general purpose, high-efficiency, reversed-phase column which features a positively charged surface modification. An excellent method development column, as it gives unique selectivity primarily for compounds that have high aromaticity, when compared to typical C18 columns. An excellent method development column as the embedded polar functional group provides alternative selectivity due to hydrogen bonding. Hydrophilic-interaction chromatography (HILIC) is a separation mode that can be used to improve the retention of extremely polar analytes. •Higher efficiency* •Increased throughput •Lower backpressure* •Fully scalable *Compared to fully-porous particles. CORTECSSOL ID-CORE PARTICLE TOMAN IPULATE SELECTV ITY 6 CH EMISTRI ES REV ERSED PH ASE 1 2 3 4 5 AU 0.00 0.01 0.02 AU 0.00 0.01 0.02 AU 0.00 0.01 0.02 12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 AU 0.00 0.01 0.02 3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0 Minutes 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 Fully Porous C18, 5 µm 4.6 x 150 mm 1.00 mL /min Alliance® HPLC CORTECS® 2.7 µm C18 4.6 x 75 mm 1.85 mL/min Alliance HPLC CORTECS 1.6 µm C18 2.1 x 50 mm 0.65 mL/min ACQUITY® UPLC® H-Class CORTECS 2.7 µm C18 3.0 x 75 mm 0.79 mL/min ACQUITY Arc UHPLC Solid-Core Particle The tightly controlled thickness of a highly porous silica layer surrounding the inner solid-core yields reproducible retention and method robustness for a wide range of sample conditions. Particle Diameter Monodisperse particle sizing provides highly permeable columns and, consequently, low backpressures. Bonding Technology Packed with solid-core particles, CORTECS Columns complement our family of particle technologies, offering unique ligand attributes that aid in method development. Packing Efficiency The increased efficiency of a solid-core particle produces more chromatographic resolution, which helps reduce the effort to separate co-eluting peaks. System: ACQUITY UPLC H-Class System with ACQUITY PDA Column: CORTECS, 2.1 x 50 mm Mobile Phase A: 10 mM Ammonium Formate pH 3 Mobile Phase B: Acetonitrile Gradient: 90%A to 65%A in 3.00 min Hold 65%A for 0.25 min 65%A to 90%A in 0.25 min Total run time: 5 min Flow rate: 0.46 mL/min Injection volume: 1 µL Column temp.: 30 °C Detection: UV at 280 nm Sample diluent: 10% Acetonitrile/90% Water Compounds: 1. Norepinephrine 2. Epinephrine 3. Dopamine 4. VMA 5. MHPG 6. Seratonin 7. DOPAC 8. 5-HIAA 9. HVA Initial method Transfer to 2.7 µm 4X faster method 2X less solvent Compatible with UHPLC systems 4X less solvent Transfer to 1.6 µm 9X faster method 15X less solvent HPLCHPLCUHPLCUPLC 9XFASTER 4XFASTER It is essential that robust methods be created quickly and easily while being compatible with a wide range of chromatographic systems. With particle sizes ideal for HPLC, UHPLC, and UPLC platforms, you can be assured a simple, and seamless transfer with consistent results across different particle sizes and column configurations. SCALABILITY AU 0.000 0.027 AU 0.000 0.045 AU 0.000 0.045 AU 0.000 0.036 AU 0.000 0.036 AU 0.000 0.024 Minutes 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 1 2 3 4 5 6 7 8 91 2 3 4 5,6 7 8 9 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 CORTECS Shield RP18 CORTECS C18+ CORTECS C18 CORTECS C8 CORTECS Phenyl CORTECS T3 7 CH EMISTRI ES COLUMN 25 CON FIGURATI O NS COLUMN Separation obtained across the two CORTECS particle sizes and three instrument classes. CORTECS 2.7 µm Columns provide maximum flexibility, designed for improved performance on HPLC and UHPLC instrumentation. CORTECS UPLC 1.6 µm Columns provide ultimate performance on ACQUITY UPLC Instrumentation. TWO FULLY SCALABLE PARTICLE SIZES TO MODERNIZE YOUR EXISTING METHODS The Truth About Solid-Core Unleash the power of your laboratory’s instrumentation Built on Waters proven solid-core technology, CORTECS 1.6 µm and 2.7 µm Columns deliver new levels of chromatographic performance.