DEHYDRATION.pdf HISTOPATH SUBJECT IN MLS
- 2. DEHYDRATING AGENTS 01 02 03 04 05 06 07 ETHYL ALCOHOL METHYL ALCOHOL BUTYL ALCOHOL ISOPROPANOL ACETONE DIOXANE ETHYLENE GLYCOL TETRAHYDROFURAN 08 ALCOHOL 09 10 TRIETHYL PHOSPHATE CARBOXYL āCellosolveā
- 3. DEHYDRATION Recommended ratio of dehydrating agent to tissue volume 10:1 ASCENDING GRADES 70%-80%-95%-95%-100% alcohol
- 4. ALCOHOL Used for routine tissue processing Increasing concentrations to avoid tissue distortion For small and delicate tissues, low concentration can MACERATE the tissues Longer storage in 70-80% may affect staining process Hastens @37 deg. C Anhydrous copper sulfate Indicator that will accelerate the process Removes water from the agent Turns BLUE when completely dehydrated
- 5. ALCOHOLS Best dehydrating agent Fast-acting Suitable for FATTY TISSUES Used for routine dehydration of urgent biopsies NON-TOXIC, but FLAMMABLE ETHYL ALCOHOL
- 6. ALCOHOLS Toxic dehydrating agent Used for blood and tissue smear preparations METHYL ALCOHOL
- 7. ALCOHOLS Suitable for PLANTS and ANIMALS BUTYL ALCOHOL Substitute for ethanol and xylene Used in microwave technique ISOPROPANOL ā¢ For tissues which do not require rapid processing