![METHODOLOGY
C. Preparation of Solid Dispersion of Curcumin-
Starch
Batches of solid dispersions for Curcumin were prepared
Drug: Porous Starch (1:0.5, 1:1, 1:2, and 1:3 w/w).
For preparation of ball milled SDs of Curcumin drug
with porous starch were subject to ball milling for 3 h
at 100 rpm[7].
Table No: 1: Different Ratios of Curcumin Solid
Dispersion using Porous Starch
Ratio Curcumin (mg) Porous Starch(gm)
[F1]1:0.5 500 0.250
[F2]1:1 500 0.500
[]F31:2 500 1
[F4]1:3 500 1.5
D. Evaluation of Solid Dispersion of
Curcumin-Porous Starch
1.Micromeritics study
2.Phase Solubility Study
3.Drug Dissolution Study
4.Infra-Red Spectroscopy
5.Crystallinity Study by
1. Powder X-ray
Diffraction(XRD)
2. Differential Scanning
Calorimetry (DSC)
10](https://image.slidesharecdn.com/developmentandcharacterizationofporousstarch-curcuminsoliddispertioninaprochtoinprovesolubilityanddi-190906071427/85/Development-and-characterization-of-porous-starch-curcumin-solid-dispertion-in-aproch-to-inprove-solubility-and-dissolution-10-320.jpg)
Development and characterization of porous starch curcumin solid dispertion in aproch to inprove solubility and dissolution
- 1. NAME OF STUDENT MS. NIKITA D. GIDDE FINAL YEAR B. PHARM NAME OF GUIDE MR. NITIN H. SALUNKHE (DEPARTMENT OF PHARMACEUTICS) 1
- 2. 1. Introduction 2. Objectives 3. Need of Present investigation 4. Drug Profile 5. Polymer Profile 6. Plan of Work 7. Methodology 8. Result and Discussion 9. Conclusion 10.References 2
- 3. Oral bioavailability of a drug depends on its solubility and dissolution rate. Therefore to increase dissolution of drug with solubility is often needed. There are the variety of new drug which having poorly aqueous solubility, out of that curcumin is one of the example Curcumin is Poorly aqueous soluble drug which consist some functional groups and starch consist some hydrophilic groups. Intermolecular bonding between the functional groups of curcumin and hydrophilic groups of polymer i.e. starch is carried out by solid dispersion method for the solubility enhancement. 3
- 4. 1. To prepare the porous starch powder. 2. To prepare and evaluate solid dispersion of curcumin-Starch. 4
- 5. There are variety of new drugs and their derivatives are available. But less than 40 % of lipophilic drugs candidates fail to reach market due to poor bioavailability, even though these drugs might exhibit potential pharmacodyanamic activities The lipophillic drug that reaches market requires a high dose to attain proper pharmacological action. The basic aim of the further formulation and development is to make that drug available at proper site of action within optimum dose. 5
- 6. 1. Curcumin is diarylheptanoid. 2. Synonyms: Indian Saffron 3. ChemicalName:1,7-bis(4-hydroxyl-3-Methoxyphenyl)-1,6-heptadiene-3,5-dion 4. Molecular Structure : 5. Molecular Formula: : C21H20O6 6. Molecular weight: 368.38g/mol 7. Melting Point : 1830C 8. Solubility : Poorly Soluble in water. Soluble in organic solvents like benzene and methanol 6
- 7. 1. Chemical Name : Cornstarch 2. Molecular Formula : C12H48O20 3. Molecular weight : 692.661g/mol 4. Structural Formula : 5. Melting Point : 2560-2580C 6. Density : 1.5g/cm3 7. pH : 6 7
- 8. Plan of work Literature surve Experimental work A. Authentication of Pure Curcumin B. Preparation of Porous Starch C. Preparation of Solid Dispersion of Curcumin-Starch D. Evaluation of Solid Dispersion of Curcumin-Porous Starch 8
- 9. B. Preparation of Porous Starch 10 gm starch + 40mL of distilled water at room temperature. 60 ml distilled water was heated to its boiling point. Starch dispersion was addedto the boiling water under rapid stirring. The temperature was steadily brought down to room temperature a translucent gel was formed. The gel was stored in excess water at 8°C overnight The gel was then subjected for solvent exchange by ethanol. The gel was equilibrated and stored in ethanol at 8°C for 48 h to maintain its porous structure. After achieving the equilibrium state, the gel was then dried at 30°C. The dried material was milled and stored Porous Starch Authentic ation of Pure Curcumin Thermal Analysis by using DSC Crystallini ty study by using XRD Compatibi lity study by using FTIR Determinati on of Calibration Curve Melting Point Solubility studies Organolep tic characteri stics A. Authentication of Pure Curcumin 9
- 10. METHODOLOGY C. Preparation of Solid Dispersion of Curcumin- Starch Batches of solid dispersions for Curcumin were prepared Drug: Porous Starch (1:0.5, 1:1, 1:2, and 1:3 w/w). For preparation of ball milled SDs of Curcumin drug with porous starch were subject to ball milling for 3 h at 100 rpm[7]. Table No: 1: Different Ratios of Curcumin Solid Dispersion using Porous Starch Ratio Curcumin (mg) Porous Starch(gm) [F1]1:0.5 500 0.250 [F2]1:1 500 0.500 []F31:2 500 1 [F4]1:3 500 1.5 D. Evaluation of Solid Dispersion of Curcumin-Porous Starch 1.Micromeritics study 2.Phase Solubility Study 3.Drug Dissolution Study 4.Infra-Red Spectroscopy 5.Crystallinity Study by 1. Powder X-ray Diffraction(XRD) 2. Differential Scanning Calorimetry (DSC) 10
- 11. D. Calibration Curve: Straight Line Equation: y= 0.0132x+0.0134 A. Organoleptic Properties Color : Yellow Odour : Earthy Taste : Bitter B. Melting Point Observed value : 1820- 1840C Reference Value : 1790-1830C C. Solubility Study Insoluble : cold water Soluble : Alcohol, Very Soluble : Ethanol 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 5 10 15 20 25 30 Absorbance Concentration(mg/ml) Sr. No. Concentration (ug/ml) Absorbance 1 0 5 0.081 2 10 0.145 3 15 0.212 4 20 0.277 5 25 0.337 6 30 0.415 11
- 12. RESULT AND DISCUSSION Batch Code Angle of Repose(0) Bulk Density(gm/cc) Tapped Density(gm/cc) Hausner Ratio Carr’s Index(%) Curcumin 37.88 0.3488 0.3564 1.194 17.25 F1 32.56 0.3643 0.418 1.143 13.06 F2 33.56 0.2753 0.316 1.147 13.5 F3 34.89 0.334 0.385 1.145 13.32 F4 35.25 0.3716 0.402 1.168 35.25 E. Flow Properties F. Saturated Solubility Study solid dispersion increase in the saturation solubility of the drugs. G. In vitro drug dissolution study The dissolution profile indicates that porous starch shows better improvement in dissolution as compared to plain drugs 12 F1 88% dissolution at the end of 120 min F2, F3, F4 approximately 98 % dissolution at the end of 120 min F2 ~90 % dissolution at the end of 60 min
- 14. RESULT AND DISCUSSION 14 H. FTIR spectroscopy The prominent peaks of curcumin exhibited at wave number, which is given in table respectively. Porous starch showed the prominent peaks at their respective position. A shift in the peaks corresponding to O–H stretching of porous starch and curcumin, respectively, indicates a strong possibility of hydrogen bonding. O-H stretching 3504.66 cm-1 C=O stretching, ketone 1618.28 cm-1 C=C 1591.27cm-1
- 15. RESULT AND DISCUSSION 15 I.Crystanillity Study a. Powder X-ray diffraction The SDs (1:1 w/w) showed a significant reduction in crystalline peaks corresponding to curcumin, indicating that crystals were transformed to an amorphous or a microcrystalline form. It means that solid- state interaction between curcumin and porous starch was observed
- 16. RESULT AND DISCUSSION 16 b. Differential scanning calorimetry (DSC) The thermograms of curcumin clearly showed sharp endothermic peaks at 173.17 ºC – 180.62 ºC.The Sharp narrow peaks were observed from the thermograms of pure curcumin, indicating the high crystallinity of the drugs. From the comparison of all thermograms, SDs (1:1 w/w) showed almost complete reduction of peak area. Thus, the results of DSC analysis confirm that curcumin is completely converted to amorphous form and favourable interactions were taking place with porous starch.
- 17. The solid dispersions of curcumin were successfully prepared by using porous starch. The developed solid dispersions were characterized with respect to solubility study, FTIR, dissolution study and crystallinity study (XRD and DSC). Curcumin-PS systems showed an improved performance as compared to curcumin. Interestingly, porous starch has been improved solubiliuty of curcumin by one fold. Thus, PS can be used as a solubility enhancer and carrier for various other drug candidates. 17
- 18. 1. M. K. Modasiya and V. M. Patel Studies on Solubility of Curcumin Int. J. of Pharm.& Life Sci (IJPLS),Vol.3, Issue 3: March:2012, 1490-1497 1490 2. DebjitBhowmik, Harish Gopinath, B. Pragati Kumar. S. Duraivel, K. P. Sampath Kumar, Oral Controlled release Drug Delivery System,Vol.1 No. 10 2012, www.thepharmajournal.com 3. Sandip Kumar, Pritam Singh, Various Techniques For Solubility Enhancement, The Pharma Innovation Journal (2016);5(1):23-28, www.ThePharmaJournal.com 4. PreethaAnand,Ajaikumar B. Kunnumakkara, Robert A. Newman, and Bharat B. Aggarwal, Bioavailability Of Curcumin : Problems and Promices, Anand et al., http://pubs.acs.org on April 2, 2009 5. Meer Tarique Ali, RiteshFule, Ajay Sav, and Purnima Amin, Porous Starch ; a Novel Carrier for Solubility Enhancement of Carmabazepin, AAPS PharmaSciTech, Vol.14, No. 3, September 2013 DOI: 10.1208/s12249-013-9985-6 6. www.wikipedia.com 7. NitinSalunkhe, Adhikrao D, Jadhav, NamdeoJadhav, Screening of drug-sericin solid dispersion for improved solubility and dissolution, N.H. Salunkhe et al./ International Journal of Biological Macromolecules xxx (2017) xxx-xxx, https://www.researchgate.net/publication/320376500 18
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