Evidence-Based Practice (EBP) Instructions for Scenario 1. Begin.docx

Evidence-Based Practice (EBP) Instructions for Scenario
1. Begin with a hypothetical clinical scenario- the father of a
client family calls seeking family therapy for his daughter who
is experiencing an eating disorder
2. ASK: Describe how you will decide on the type of question
you are researching. Be specific in your decision-making
process and what criteria you will use. For example, will you
use one of the classifications of questions discussed in your text
in the Defining the Purpose of the Search section to formulate
your questions? Convert the issue into an answerable question.
Write the question and indicate what type of clinical question it
is (a who, what, where, when or why question). Explain why.
3. ACQUIRE: Describe how you will find research articles to
answer your question. Pay particular attention to how you will
do this without having access to a university library. Practice
locating research articles that address the issue. Describe what
resources and databases you might use to locate various types of
articles for the issue.
4. APPRAISE: Describe how you will determine the quality of
the research, to whom the findings apply and in what contexts
they are appropriate. Select one research study that you found
related to the clinical issue and complete an evaluation of it.
Describe what you found to assess the quality of this article
using your strategies for evaluating research.
5. APPLY: Describe how you will integrate the research
findings with your experience to date and your hypothetical
client population and clinical context. In other words, envision
who you might work with and where you might work with
them, and then describe how you will decide on the most
effective way to use research findings in your therapy with such
clients. What kinds of information do you anticipate pulling
from research articles to guide your work with such clients?
Explain, in detail, how you would apply the research findings to
your work with your hypothetical clients in your hypothetical

clinical context (assuming the study is of quality). Then,
clarify all relevant diversity/contextual relation variables
associated with this situation and indicate how they may impact
the utility of the evidence you found (e.g., if the study used a
European or an American sample, will it apply to your Latina
client?).
6. ANALYZE AND ADJUST: Describe what methods you will
use to assess the effectiveness of the interventions you might
pull from the studies you found. Considering that the research-
based interventions you found may not always be effective,
explain what you would do in such situations. Identify the
specific measures, tools, and procedures you would use to
assess the effectiveness of your EBP interventions with these
clients.
Length: 8 pages
Studies of yeast respiration with the oxygen electrode
Aims
You will measure compare the rates of respiration by yeast of
sugars at different conditions using the oxygen electrode.
Good laboratory practice is essential: no eating or drinking;
wear lab coats at all times; handle chemicals carefully and with
gloves; read, understand and reflect about the experiment.
Introduction
Under aerobic conditions sugars are respired to carbon dioxide
and water via the glycolytic pathway and the citric acid cycle.
The respiration of a yeast suspension is conveniently monitored
by following the decline in the concentration of dissolved
oxygen using an oxygen electrode. It is essential to starve the

yeast suspension by bubbling a stream of air through it
overnight to deplete endogenous reserves of glycogen,
otherwise a high rate of respiration is observed before the
substrate (eg. a sugar) is added. Even then, a small rate of
respiration is usually observed due to metabolism of remaining
endogenous reserves. It is usual practice to estimate this, the
endogenous rate, and to apply a correction for it.
Materials:
· 7.5 mL 0.05M K phosphate buffer pH 5.0, saturated with air at
30C by bubbling.
30C by bubbling.
30C by bubbling.
· 5 mL 1% (w/v) suspension of fresh yeast in the buffer above,
starved by bubbling with air at 30C for at least 4 hours.
· Glucose (0.16M), Galactose (0.16M), Ethanol (0.16M),
Sodium Azide (0.16M)
Procedure
1. Pipette 1500 L of air-saturated buffer into the reaction vessel
of an oxygen electrode. Note the temperature of the water bath
in which the buffer is immersed; you will need this for a
subsequent calculation. If you do not have a thermometer, note
the temperature as 30C.
2. Calibrate the electrode by turning the dial till it reaches 100.
If the electrode reading fluctuates wildly or drifts steadily
downwards, consult a member of staff.
3. Add 1000 μL of the 10 mg/mL yeast suspension to the
reaction vessel, and start recording the data every 5 seconds.
5. When you reach 100 seconds, inject 25 L of 0.16 M glucose
solution through the vent to the electrode chamber using a
Hamilton syringe and allow the data to accumulate until 200

seconds, at this time inject 25 L of 0.16 M sodium azide
Hamilton syringe and allow the data to accumulate the end of
the run (360 seconds). Ensure that the needle enters the
electrode chamber.
6. Pipette out the contents of the reaction chamber with a
dropping pipette and wash out the reaction chamber three times
with distilled water.
7. Determine respiration rates before and after the addition of
25 L of 0.16 M solution of galactose by repeating steps 1-3.
9. Pipette 1500 L of air-saturated buffer (pH 1) into the reaction
vessel of an oxygen electrode. Note the temperature of the water
bath in which the buffer is immersed; you will need this for a
subsequent calculation. If you do not have a thermometer, note
the temperature as 30C.
10. Calibrate the electrode by turning the dial till it reaches
100. If the electrode reading fluctuates wildly or drifts steadily
electrode chamber.

16. Pipette 1500 L of air-saturated buffer (pH 1) into the
reaction vessel of an oxygen electrode. Note the temperature of
the water bath in which the buffer is immersed; you will need
this for a subsequent calculation. If you do not have a
thermometer, note the temperature as 30C.
17. Calibrate the electrode by turning the dial till it reaches
100. If the electrode reading fluctuates wildly or drifts steadily
electrode chamber.

seconds
Glucose pH 5
Galactose
pH 5
Glucose pH 1
Galactose
pH 1
Glucose pH 9
Galactose
pH 9
0
5
10
15

355
360
For your report you need to plot and obtain:
1. Plot out your results using excel with the time scale in
seconds. Use a different symbol for each substrate and mark the
point on the plot where the substrate was added.
2. Determine the slope of the linear part of each curve (using
the slope function of EXCEL) before and after the addition of
substrate making use of as many experimental points as
possible. Where the rate increases steadily after the addition of
substrate take the maximum rate achieved.
3. Correct the rate obtained following the addition of substrate
for the endogenous rate, ie. Subtract the rate obtained before
substrate was added. Your rates should be in s-1.
4. Calculate the rate of respiration of each substrate in mol O2
consumed per min per mg wet weight of yeast as follows:

Rate in mol/min/mg = (corrected slope x 60 x 0.237 x 3)/(100 x
10)
(Multiplying by 60 s/min converts the rate from s-1 to min-1)
(Multiplying by 0.237 x 3 and dividing by 100 converts the rate
from a change in electrode reading/min to mol O2
consumed/min since the electrode was set to read 100 on buffer
saturated with air at 30C which contains 0.237 mol O2 per mL
or 0.237 x 3 mol in the 3 mL (3000 L) system)
(Division by 10 converts the rate to mol/min/mg yeast since 10
mg of yeast was added to the system, ie. 1 mL (1000 L) of a 10
mg/mL suspension.)
You should comment in your discussion mitochondrial diseases,
how monitoring oxygen is important, respiratory chain blockers.
Your full write up experiments should be written up under the
headings below, as appropriate. You will still need to keep a
good lab notebook but there will be a formal write up to hand in
two weeks after the lab class.
The turnitin report will be marked as:
Abstract: 5 marks
Introduction: 15marks
Safety Audit: 5 marks
Materials and methods: 5 marks
Results: 45 marks
Conclusion and Discussion: 20 marks
Referencing: 5 marks
GUIDELINES ON TURNITIN REPORTS
The format for writing a lab report generally follows the
standardized approach outlined here, but you may find some
assignments issued to you on different modules have slightly

different requirements. Always check with the person setting
the assessment if you are unsure! For this module, the full
report should follow the structure outlined below.
TITLE : This is a concise (one sentence) heading that
summarizes what the report is about.
ABSTRACT: This is conventionally a 200 word summary of the
report. In the first sentence, the research question should be
introduced and in the second sentence the methodology should
be described. The major results of the study should be reported
in the next sentence and your concluding statement should
include an interpretation of the impact and significance of your
result. A selection of published abstracts written by some of
your lecturers are available on Blackboard under ‘Learning
Materials’- check them out! You will see that there are various
ways of writing an abstract and this is ultimately dictated by the
journal in which the work is published. Being able to write a
concise summary of your work with adherence to word counts is
excellent academic writing practice.
INTRODUCTION: This section is often the most variable
between different assessments. The introduction should be 1
side of A4 (12pt font) and aims to introduce the research
question being addressed whilst putting it into context of what
is already known about the subject (i.e. the background
information). In brief, the introduction should include;
1) A description of the research question and a summary of
what is currently known about the research question (for
example, if you are investigating the effect of drug X on protein
X, then what is currently known about drug X and protein X?).
This is usually the longest section of the introduction.
2) A statement concerning the aims of your study and what
methods will be used to address the aims (for example, ‘We aim
to assess the effect of drug X on the activity of protein X by
measuring protein X activity via a colorimetric assay utilizing
spectrophotometry’)
SAFETY AUDIT: Before starting, read through the entire
experiment and check all materials and solvents involved for

hazardous properties, making brief notes. A safety audit should
include the risk phrases and numbers as well as the safety
phrases and numbers for each material. You can find the in the
MSDS (material safety datasheet for each chemical).
MATERIALS AND METHODS: This should be a summary of
the experimental procedure, written in your own words in the
third person passive, e.g. "sodium chloride (20 g) was added to
a stirred solution of silver nitrate (2 g) in water (50 ml). The
white precipitate was collected, washed with water (20 ml), and
dried at 70 ˚C" etc. It must not be copied from the laboratory
manual. Note that quantities are placed in parentheses () after
the name of the substance to which they refer, i.e. "water (50
ml)" is correct, while "50 ml of water" is wrong. You should
NOT simply cut and paste the full protocol listed in the module
book! Occasionally, you may be asked to put ‘as per the module
book’ with an appropriate citation, but not in this module! For
the reports associated with this module, you should produce a
short and concise summary of the protocol written in past tense
and remember, it should contain enough practical detail to
enable anyone else to successfully repeat the work. Important
information that should be incorporated in this section includes
the citation of volumes and concentrations of all reactants used,
the temperature and pH used, the timings of reaction etc and
any other important variables (and how they were controlled).
RESULTS: In this section you should show your results (using
graphs, tables, photographs etc) AND describe your results. It is
not enough to simply put a graph in this section without
explaining what it shows! You don’t need to interpret or explain
the results in this section (that is for the discussion section!),
just ‘describe what you see’ and identify any trends/patterns.
Present your quantitative (or indeed qualitative) results in the
clearest possible way with adequate linking of each part of the
experiment (if more than one part) in a logical sequence. Ensure
that tables and graphs are self-explanatory and provide all the
information necessary to allow interpretation of the data
without reference to the text. Tables should have an appropriate

title above them for example ‘Table 1- Protein X activity at
different concentrations of drug X’ whereas figures (graphs,
pictures, photographs) should have an appropriate title below
them for example ‘Figure 1- Protein X activity at different
concentrations of drug X’. Graphs also need a clear heading for
example ‘A graph to show the relationship between absorbance
at 495nm of protein X and drug X concentrations’ and both x
and y axes should be appropriately labelled and include a
citation of units of measurements. Remember, the Y axis values
should be the dependent variable, i.e., what was measured
during the experiment or calculated at the end of the
experiment. The X axis values are the independent variables,
i.e. the ‘conditions’ that are actually known, set-up and
controlled in the experiment. Always compare your analytical
data (e.g. melting points) with published values, and quote the
source of your information in full, i.e. authors name and
initials, title of the journal or book, publisher (if a book), year,
volume, and page number.
DISCUSSION and CONCLUSSION: In this section you should
explain and discuss your results with reference to the
underlying scientific principles and other research in the field.
You should try to include a discussion of; 1) Any overall trends
and patterns in the results and how/why anomaly formation
might have occurred. A thorough scientific explanation of the
results should be offered and you should link this to scientific
literature (including textbooks and scientific publications).
What scientific evidence exists to support your findings? Is
there any scientific evidence that contradicts your findings?
What is the scientific explanation of this? 2) How the results fit
in with the original aims. Did your results address the aims of
the study? If your results are not what you expect- what could
be the scientific explanation? 3) Errors, limitations,
improvements and alternative approaches. Were there any
difficulties encountered during the lab work? How accurate and
precise were your methods/equipment? Were all variables
sufficiently controlled? Were there any limitations of the

methods used? Could any of these factors have affected the
reliability and variability of your data? How could you have
improved the study? Are there any other alternative approaches
(such as those used in industry, clinical/research labs) that
could be used to address the same research aims? 4) Future
investigations How could you extend the study to understand
the research question in more detail?. CONCLUSSION: You
should give a brief, justified conclusion/summary of your
results and their significance (3-4 lines only).
BIBLIOGRAPHY: This section goes at the end of the write up.
Any reading material that you have consulted during the write-
up should be cited in text and in the bibliography section at the
end of the report using the Harvard System method- full details
of how to reference appropriately can be found in the Study
Skills module book. In brief, in the main body of the text you
should cite the author surnames (if only one or two authors) and
year published (Smith, 2010 or Smith & Jones, 2010). If there
are more than three authors, you cite the first author surname
followed by ‘et al’ and the year published (Smith et al, 2010).
For example; In the bibliography section at the end, the full
citation should be provided, and these should be listed in
alphabetical order by first author surname. Different
information needs to be listed, depending on what kind of
source you used for your information. In brief, the minimum
you should cite is;
For journal articles cite author(s) surname(s), initial(s), year of
publication, title, journal, volume, pages.
For example Smith,J. (1995). Amylases in plants. Journal of
Botany 54, p123-456.
For books cite editor(s) name, initial(s), year of publication,
title, edition, place of publication and publishers.
For example Jones, B. and Evans, C. (2004). Carbohydrate
Biochemistry. 20th edition. New York: Academic Press.
For websites cite author(s) surname(s), initial(s), title, year of
publication, publisher (if appropriate), available at (followed by
full web site address) accessed on: (followed by date you

accessed the site as some internet material may change or be
updated
Bloggs, F. A guide to enzymes and their uses [on-line].
University of Salford, 2005. Available at:
http://www.enzymes.nature.uses.org.html. [Accessed on 30th
Sept. 2011].
More detailed information and guidelines concerning
referencing via the Harvard method can be found in the Study
Skills module book and on the Blackboard site for this module
under ‘Learning Materials’.
A WARNING ABOUT PLAGIARISM
Plagiarism is very serious example of unfair practice. It can be
defined as the compilation, incorporation and/or documentation
by students of work which is NOT their own, in all types of
work submitted for assessment (e.g. essays, theses,
dissertations, laboratory write-ups etc). When you write up your
coursework or similar you must always acknowledge any books,
texts, papers, articles or information sources that you have used
and any ideas or work that are not your own. You should not
take any text word-for-word from any source- the University
has access to advanced technology that compares your work to
that of all other student submissions all over the world as well
as internet sources and textbooks etc. IF YOU COPY
ANYTHING FROM ANYWHERE, WORD FOR WORD THEN
OUR SOFTWARE WILL DETECT THIS AND YOU WILL BE
INVESTIGATED FOR ACADEMIC MISCONDUCT!!!!!!!!!
Always put everything into your own words. When you have to
submit your report via the TURNITIN system on Blackboard,
you can submit a ‘draft’ version of your report before you
submit your final report which allows you to check how similar
your report is to other sources. Please use this draft submission
facility!
Examples of plagiarism
1. Copying and submitting other students’ work or giving
your work to someone else is plagiarism (we can’t prove who

copied from who!). If you were not there for the lab, you cannot
submit the report.
2. Working together on coursework (unless otherwise stated)
and submitting the same or very similar work is plagiarism!
3. Submission of a joint/group piece of work (as directed by a
member of staff) for assessment is not plagiarism unless there is
evidence that the work is not your own (for example putting
your name on a piece of work that you did not actually
contribute to).
4. Using the same data as your lab partner (when collected
together) is not plagiarism. Using another groups data without
permission of the member of staff leading the lab is plagiarism.
5. ‘Sharing’ and submitting the same figures with your lab
partner is plagiarism. Even when you have the same data as
your peers you should create your own graphs and produce your
own labels/titles etc.
6. Copying work (even if a single sentence) word for word
from any source is plagiarism. If you need to do this (for
example, when providing a scientific definition or quotation)
then use quotation marks but you shouldn’t do this frequently.
7. Not citing in text or not having a bibliography is
plagiarism. At L4, we may ‘forgive’ a forgotten or incorrect
citation since you are in the process of learning how to
reference appropriately. You will, however, lose marks for this
and the consequences or inadequate referencing are much more
severe at L5. Get into good habits now!
8. Not attending the practical session and submitting the
work (in class or assessment) is plagiarism.
9. Do NOT use the module handbook to copy the
protocol/procedure, this should be in your OWN words
Please refer to the University’s Academic Quality Assurance
booklet for the policy and guidelines on plagiarism. You have
been warned!
IMPORTANT SAFETY INFORMATION
Safety is of paramount importance in the laboratory. Before
you begin practical work you will receive a lecture on key

aspects of safety in the laboratories. You must wear safety
spectacles at all times. Tie back long hair, and do not wear
open-toed sandals. You must wear a lab coat at all times. You
must not eat, drink, run, listen to personal stereos or use mobile
phones in the laboratory. Be aware of the danger of fires. Many
solvents are both volatile and flammable and may ignite if the
liquid or the vapour comes in contact with flames or hot
surfaces. Do not use a Bunsen burner until you are absolutely
sure that no one is using a flammable solvent nearby, and
always turn it off immediately after use. Be aware of the danger
of cuts. Together with fires, cuts are the most common
accidents we encounter. Check your glassware for breakages or
sharp edges, and check carefully for cracks or 'star-cracks' in
glassware which is to be used under suction (e.g. filtration
flasks), or under pressure (e.g. chromatography columns). Keep
ground glass joints clean so that they do not stick. Handle
sample vials carefully – they can easily break when the caps are
being fitted. All accidents must be reported to a member of
academic staff. This is so that we can see that they are properly
dealt with, and take steps to prevent repetitions. In cases of skin
contact, wash thoroughly with soap and water. If skin blisters
contact a demonstrator. In case of skin puncture, e.g. glass cuts,
wash thoroughly under a running tap for several minutes and
seek a demonstrator's advice. In cases of eye contact, rinse eyes
with an eyewash and contact a demonstrator immediately. In
cases of oral intake, contact the demonstrator immediately. You
must be aware of the location of safety equipment. This
includes eye-wash stations, fire extinguishers, fire blankets, and
drench showers. Treat all chemicals with caution. Do not get
any compound, especially one for which no specific safety
information is available, on your hands or skin, and do not
breathe dust or vapours. Use protective clothing, e.g. gloves,
when necessary and only handle more dangerous or unpleasant
compounds in fume cupboards. Do not leave spillages, alert a
demonstrator or technician when anything is spilt.

pH5 glucosesecondsGlucoseGlucoseglucose average
stdev098.498.5598.4750.106066017259898.1598.0750.1060660
1721098.298.3598.2750.10606601721597.497.5597.4750.10606
601722097.998.0597.9750.1060660172259797.1597.0750.10606
601723097.297.3597.2750.10606601723597.998.0597.9750.106
0660172409898.1598.0750.10606601724597.998.0597.9750.106
06601725097.497.5597.4750.1060660172559898.1598.0750.106
06601726098.798.8598.7750.10606601726598.298.3598.2750.1
0606601727098.498.5598.4750.10606601727597.397.4597.3750
.10606601728097.397.4597.3750.10606601728598.198.2598.17
50.10606601729097.998.0597.9750.10606601729598.598.6598.
5750.106066017210097.897.9597.8750.1060660172glucose
added10597.897.9597.8750.106066017211097.597.6597.5750.1
06066017211597.998.0597.9750.106066017212098.598.6598.57
50.106066017212598.298.3598.2750.106066017213097.597.659
7.5750.106066017213597.998.0597.9750.106066017214097.497
.5597.4750.106066017214597.697.7597.6750.106066017215097
.297.3597.2750.10606601721559898.1598.0750.1060660172160
97.397.4597.3750.106066017216597.897.9597.8750.106066017
217097.697.7597.6750.106066017217597.297.3597.2750.10606
6017218097.998.0597.9750.106066017218597.897.9597.8750.1
06066017219097.797.8597.7750.106066017219597.897.9597.87
50.106066017220097.797.8597.7750.1060660172Azide
added20597.497.5597.4750.10606601722109797.1597.0750.106
066017221597.297.3597.2750.106066017222096.696.7596.6750
.106066017222596.496.5596.4750.106066017223096.796.8596.
7750.106066017223596.997.0596.9750.106066017224096.796.8
596.7750.10606601722459797.1597.0750.106066017225097.59
7.6597.5750.106066017225596.496.5596.4750.10606601722609
7.297.3597.2750.106066017226597.597.6597.5750.1060660172
27096.997.0596.9750.106066017227596.696.7596.6750.106066
017228096.596.6596.5750.106066017228596.396.4596.3750.10
6066017229096.896.9596.8750.106066017229596.296.3596.275
0.106066017230096.596.6596.5750.106066017230596.997.0596
.9750.106066017231096.496.5596.4750.106066017231596.796.

8596.7750.106066017232097.197.2597.1750.106066017232596.
896.9596.8750.106066017233096.596.6596.5750.106066017233
596.997.0596.9750.106066017234097.197.2597.1750.10606601
7234596.997.0596.9750.106066017235096.896.9596.8750.1060
66017235597.297.3597.2750.106066017236096.796.8596.7750.
1060660172
glucose average 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601 717798615
0.10606601717798615 0.10606601717798615
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0.10606601717798615 0.10606601717798615
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0.10606601717798615 0.10606601717798615

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0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
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0.10606601717798615 0.10606601717798615
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0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
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0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0 5 10 15 20 25 30
35 40 45 50 55 60 65 70 75 80 85 90
95 100 105 110 115 120 125 130 135 140 145
150 155 160 165 170 175 180 185 190 195 200
205 210 215 220 225 230 235 240 245 250 255
260 265 270 275 280 285 290 295 300 305 310
315 320 325 330 335 340 345 350 355 360
98.475000000000009 98.075000000000003
98.275000000000006 97.475000000000009
97.975000000000009 97.075000000000003
97.275000000000006 97.975000000000009
98.075000000000003 97.975000000000009
97.475000000000009 98.075000000000003
98.775000000000006 98.275000000000006
98.475000000000009 97.375 97.375
98.174999999999997 97.975000000000009
98.575000000000003 97.875 97.875
97.575000000000003 97.975000000000009
98.575000000000003 98.275000000000006
97.575000000000003 97.975000000000009
97.475000000000009 97.674999999999997
97.275000000000006 98.075000000000003 97.375
97.875 97.674999999999997 97.275000000000006
97.975000000000009 97.875 97.775000000000006
97.875 97.775000000000006 97.475000000000009
97.075000000000003 97.275000000000006
96.674999999999997 96.475000000000009
96.775000000000006 96.975000000000009
96.775000000000006 97.075000000000003

97.575000000000003 96.475000000000009
97.275000000000006 97.575000000000003
96.975000000000009 96.674999999999997
96.575000000000003 96.375 96.875
96.275000000000006 96.575000000000003
96.975000000000009 96.475000000000009
96.775000000000006 97.174999999999997 96.875
96.575000000000003 96.975000000000009
97.174999999999997 96.975000000000009 96.875
97.275000000000006 96.775000000000006
Sheet pH5
glucosesecondsGlucose098.475598.0751098.2751597.4752097.9
752597.0753097.2753597.9754098.0754597.9755097.4755598.0
756098.7756598.2757098.4757597.3758097.3758598.1759097.9
759598.57510097.875rate corrected:0.0098-0.0018use the
modular value, ignore the sign
minus10597.8750.00811097.57511597.975Rate in mmol/min/mg
= (corrected slope x 60 x 0.237 x 3)/(100 x
10)(0.008*60*0.237*3)/(100*10)12098.5750.00034128umol/mi
n/mg12598.27513097.57513597.97514097.47514597.67515097.
27515598.07516097.37516597.87517097.67517597.27518097.9
7518597.87519097.77519597.87520097.77520597.47521097.07
521597.27522096.67522596.47523096.77523596.97524096.775
24597.07525097.57525596.47526097.27526597.57527096.9752
7596.67528096.57528596.37529096.87529596.27530096.57530
596.97531096.47531596.77532097.17532596.87533096.575335
96.97534097.17534596.97535096.87535597.27536096.775
Glucose 0 5 10 15 20 25 30 35 40 45 50
55 60 65 70 75 80 85 90 95 100 105
110 115 120 125 130 135 140 145 150 155 160
165 170 175 180 185 190 195 200 205 210 215
220 225 230 235 240 245 250 255 260 265 270
275 280 285 290 295 300 305 310 315 320 325
330 335 340 345 350 355 360 98.475000000000009
98.075000000000003 98.2750000000000 06
97.475000000000009 97.975000000000009

97.075000000000003 97.275000000000006
97.975000000000009 98.075000000000003
97.975000000000009 97.475000000000009
98.075000000000003 98.775000000000006
98.275000000000006 98.475000000000009 97.375
97.375 98.174999999999997 97.975000000000009
98.575000000000003 97.875 97.875
97.575000000000003 97.975000000000009
98.575000000000003 98.275000000000006
97.575000000000003 97.975000000000009
97.475000000000009 97.674999999999997
97.275000000000006 98.075000000000003 97.375
97.875 97.674999999999997 97.275000000000006
97.975000000000009 97.875 97.775000000000006
97.875 97.775000000000006 97.475000000000009
97.075000000000003 97.275000000000006
96.674999999999997 96.475000000000009
96.775000000000006 96.975000000000009
96.775000000000006 97.075000000000003
97.575000000000003 96.475000000000009
97.275000000000006 97.575000000000003
96.975000000000009 96.674999999999997
96.575000000000003 96.375 96.875
96.275000000000006 96.575000000000003
96.975000000000009 96.475000000000009
96.775000000000006 97.174999999999997 96.875
96.575000000000003 96.975000000000009
97.174999999999997 96.975000000000009 96.875
97.275000000000006 96.775000000000006
Glucose 15 20 25 30 35 40 45 50 55 60
65 70 75 80 85 90 95 100 105 110 115
120 125 97.475000000000009 97.975000000000009
97.075000000000003 97.275000000000006
97.975000000000009 98.075000000000003
97.975000000000009 97.475000000000009
98.075000000000003 98.775000000000006

98.275000000000006 98.475000000000009 97.375
97.375 98.174999999999997 97.975000000000009
98.575000000000003 97.875 97.875
97.575000000000003 97.975000000000009
98.575000000000003 98.275000000000006 150
155 160 165 170 175 180 185 190 195 200
97.275000000000006 98.075000000000003 97.375
97.875 97.674999999999997 97.275000000000006
97.975000000000009 97.875 97.775000000000006
97.875 97.775000000000006
pH9 glucose secondsGlucoseGlucoseglucose average
stdev0110110.15110.0750.10606601725110.5110.65110.5750.1
06066017210106.5106.65106.5750.106066017215104104.15104
.0750.106066017220102.8102.95102.8750.10606601722598.798
.8598.7750.10606601723095.595.6595.5750.10606601723593.3
93.4593.3750.10606601724091.291.3591.2750.10606601724589
.990.0589.9750.10606601725088.388.4588.3750.106066017255
85.885.9585.8750.10606601726085.485.5585.4750.1060660172
6584.384.4584.3750.10606601727082.983.0582.9750.10606601
727581.681.7581.6750.10606601728086.486.5586.4750.106066
01728579.879.9579.8750.10606601729078.878.9578.8750.1060
6601729577.978.0577.9750.106066017210076.876.9576.8750.1
060660172glucose
added10571.771.8571.7750.106066017211071.671.7571.6750.1
06066017211571.271.3571.2750.10606601721207171.1571.075
0.106066017212571.671.7571.6750.106066017213071.571.6571
.5750.106066017213571.171.2571.1750.106066017214070.971.
0570.9750.106066017214570.770.8570.7750.106066017215070.
470.5570.4750.106066017215570.170.2570.1750.106066017216
070.470.5570.4750.106066017216569.869.9569.8750.10606601
7217069.769.8569.7750.106066017217569.469.5569.4750.1060
66017218069.269.3569.2750.106066017218569.169.2569.1750.
10606601721906969.1569.0750.106066017219569.169.2569.17
50.106066017220068.668.7568.6750.1060660172Azide
added20579.579.6579.5750.106066017221079.179.2579.1750.1
06066017221578.378.4578.3750.106066017222077.377.4577.37

50.106066017222577.177.2577.1750.106066017223076.977.057
6.9750.106066017223575.976.0575.9750.106066017224075.375
.4575.3750.10606601722457575.1575.0750.106066017225074.8
74.9574.8750.106066017225573.373.4573.3750.1060660172260
72.772.8572.7750.106066017226571.972.0571.9750.106066017
227070.470.5570.4750.106066017227569.970.0569.9750.10606
6017228068.868.9568.8750.106066017228569.469.5569.4750.1
06066017229068.969.0568.9750.106066017229568.268.3568.27
50.106066017230067.968.0567.9750.106066017230567.467.556
7.4750.106066017231067.267.3567.2750.106066017231566.866
.9566.8750.106066017232066.266.3566.2750.106066017232565
.966.0565.9750.106066017233065.165.2565.1750.10606601723
3564.965.0564.9750.106066017234064.364.4564.3750.1060660
17234564.164.2564.1750.106066017235063.863.9563.8750.106
066017235563.263.3563.2750.106066017236062.862.9562.8750
.1060660172
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.106066 01717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798113
0.10606601717798113 0.10606601717798113
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10 606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798113 0.10606601717798113
0.10606601717798113 0 5 10 15 20 25 30
35 40 45 50 55 60 65 70 75 80 85 90
95 100 105 110 115 120 125 130 135 140 145
150 155 160 165 170 175 180 185 190 195 200
205 210 215 220 225 230 235 240 245 250 255
260 265 270 275 280 285 290 295 300 305 310
315 320 325 330 335 340 345 350 355 360
110.075 110.575 106.575 104.075 102.875
98.775000000000006 95.575000000000003 93.375
91.275000000000006 89.975000000000009 88.375
85.875 85.475000000000009 84.375
82.975000000000009 81.674999999999997
86.475000000000009 79.875 78.875
77.975000000000009 76.875 71.775000000000006
71.674999999999997 71.275000000000006
71.075000000000003 71.674999999999997
71.575000000000003 71.174999999999997
70.975000000000009 70.775000000000006
70.475000000000009 70.174999999999997

70.475000000000009 69.875 69.775000000000006
69.475000000000009 69.275000000000006
69.174999999999997 69.075000000000003
69.174999999999997 68.674999999999997
79.575000000000003 79.174999999999997 78.375
77.375 77.174999999999997 76.975000000000009
75.975 000000000009 75.375 75.075000000000003
74.875 73.375 72.775000000000006
71.975000000000009 70.475000000000009
69.975000000000009 68.875 69.475000000000009
68.975000000000009 68.275000000000006
67.975000000000009 67.475000000000009
67.275000000000006 66.875 66.275000000000006
65.975000000000009 65.174999999999997
64.975000000000009 64.375 64.174999999999997
63.875 63.275000000000006 62.875
Sheet pH9 glucose
secondsGlucose0110.0755110.57510106.57515104.07520102.87
52598.7753095.5753593.3754091.2754589.9755088.3755585.87
56085.4756584.3757082.9757581.6758086.4758579.8759078.87
10)(0.008*60*0.237*3)/(100*10)12071.0750.00034128umol/mi
n/mg12571.67513071.57513571.17514070.97514570.77515070.
47515570.17516070.47516569.87517069.77517569.47518069.2
7518569.17519069.07519569.17520068.67520579.57521079.17
521578.37522077.37522577.17523076.97523575.97524075.375
24575.07525074.87525573.37526072.77526571.97527070.4752
7569.97528068.87528569.47529068.97529568.27530067.97530
567.47531067.27531566.87532066.27532565.97533065.175335
64.97534064.37534564.17535063.87535563.27536062.875
Glucose 0 5 10 15 20 25 30 35 40 45 50
55 60 65 70 75 80 85 90 95 100 105
110 115 120 125 130 135 140 145 150 155 160

165 170 175 180 185 190 195 200 205 210 215
220 225 230 235 240 245 250 255 260 265 270
275 280 285 290 295 300 305 310 315 320 325
330 335 340 345 350 355 360 110.075 110.575
106.575 104.075 102.875 98.775000000000006
95.575000000000003 93.375 91.275000000000006
89.975000000000009 88.375 85.875
85.475000000000009 84.375 82.975000000000009
81.674999999999997 86.475000000000009 79.875
78.875 77.975000000000009 76.875
71.775000000000006 71.674999999999997
71.275000000000006 71.075000000000003
71.674999999999997 71.575000000000003
71.174999999999997 70.975000000000009
70.775000000000006 70.475000000000009
70.174999999999997 70.475000000000009 69.875
69.775000000000006 69.475000000000009
69.275000000000006 69.174999999999997
69.075000000000003 69.174999999999997
68.674999999999997 79.575000000000003
79.174999999999997 78.375 77.375
77.174999999999997 76.975000000000009
75.975000000000009 75.375 75.075000000000003
74.875 73.375 72.775000000000006
71.975000000000009 70.475000000000009
69.975000000000009 68.875 69.475000000000009
68.975000000000009 68.275000000000006
67.975000000000009 67.475000000000009
67.275000000000006 66.875 66.275000000000006
65.975000000000009 65.174999999999997
64.975000000000009 64.375 64.174999999999997
63.875 63.275000000000006 62.875 Glucose 15
20 25 30 35 40 45 50 55 60 65 70 75
80 85 90 95 100 105 110 115 120 125
104.075 102.875 98.775000000000006
95.575000000000003 93.375 91.275000000000006

89.975000000000009 88.375 85.875
85.475000000000009 84.375 82.975000000000009
81.674999999999997 86.475000000000009 79.875
78.875 77.975000000000009 76.875
71.775000000000006 71.674999999999997
71.275000000000006 71.075000000000003
71.674999999999997 150 155 160 165 170 175
180 185 190 195 200 70.475000000000009
70.174999999999997 70.475000000000009 69.875
69.775000000000006 69.475000000000009
69.275000000000006 69.174999999999997
69.075000000000003 69.174999999999997
68.674999999999997
pH1 glucose secondsGlucoseGlucoseglucose average
stdev0107107.15107.0750.10606601725109.7109.85109.7750.1
06066017210107.6107.75107.6750.106066017215102.5102.651
02.5750.10606601722099.9100.0599.9750.10606601722597.297
.3597.2750.10606601723095.996.0595.9750.10606601723593.4
93.5593.4750.10606601724091.291.3591.2750.10606601724591
91.1591.0750.10606601725089.189.2589.1750.10606601725587
.787.8587.7750.10606601726086.686.7586.6750.106066017265
85.185.2585.1750.10606601727084.584.6584.5750.1060660172
7582.782.8582.7750.10606601728081.681.7581.6750.10606601
728579.779.8579.7750.10606601729078.878.9578.8750.106066
01729578.278.3578.2750.106066017210077.978.0577.9750.106
0660172glucose
added10581.481.5581.4750.10606601721108282.1582.0750.106
066017211581.681.7581.6750.106066017212081.381.4581.3750
.106066017212580.880.9580.8750.106066017213080.380.4580.
3750.106066017213579.779.8579.7750.106066017214079.179.2
579.1750.106066017214578.778.8578.7750.106066017215078.4
78.5578.4750.106066017215578.278.3578.2750.1060660172160
78.178.2578.1750.106066017216578.278.3578.2750.106066017
217078.478.5578.4750.106066017217578.378.4578.3750.10606
6017218078.878.9578.8750.106066017218579.179.2579.1750.1
06066017219078.778.8578.7750.106066017219579.479.5579.47

50.106066017220079.379.4579.3750.1060660172Azide
added20576.977.0576.9750.106066017221076.576.6576.5750.1
06066017221576.476.5576.4750.106066017222075.976.0575.97
50.106066017222575.875.9575.8750.106066017223075.775.857
5.7750.106066017223574.975.0574.9750.106066017224074.674
.7574.6750.106066017224574.174.2574.1750.106066017225073
.773.8573.7750.106066017225573.173.2573.1750.10606601722
6072.872.9572.8750.106066017226572.372.4572.3750.1060660
17227072.172.2572.1750.106066017227572.572.6572.5750.106
066017228072.772.8572.7750.106066017228573.173.2573.1750
.106066017229073.473.5573.4750.106066017229574.374.4574.
3750.106066017230074.774.8574.7750.106066017230575.375.4
575.3750.106066017231076.176.2576.1750.106066017231576.9
77.0576.9750.106066017232077.177.2577.1750.1060660172325
77.978.0577.9750.106066017233077.677.7577.6750.106066017
233577.978.0577.9750.106066017234077.677.7577.6750.10606
6017234578.278.3578.2750.106066017235078.278.3578.2750.1
06066017235577.777.8577.7750.106066017236077.677.7577.67
50.1060660172
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.106066 01717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10 606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0 5 10 15 20 25 30
35 40 45 50 55 60 65 70 75 80 85 90
95 100 105 110 115 120 125 130 135 140 145
150 155 160 165 170 175 180 185 190 195 200
205 210 215 220 225 230 235 240 245 250 255
260 265 270 275 280 285 290 295 300 305 310
315 320 325 330 335 340 345 350 355 360
107.075 109.77500000000001 107.675 102.575
99.9750 00000000009 97.275000000000006
95.975000000000009 93.475000000000009
91.275000000000006 91.075000000000003
89.174999999999997 87.775000000000006
86.674999999999997 85.174999999999997
84.575000000000003 82.775000000000006
81.674999999999997 79.775000000000006 78.875
78.275000000000006 77.975000000000009

81.475000000000009 82.075000000000003
81.674999999999997 81.375 80.875 80.375
79.775000000000006 79.174999999999997
78.775000000000006 78.475000000000009
78.275000000000006 78.174999999999997
78.275000000000006 78.475000000000009 78.375
78.875 79.174999999999997 78.775000000000006
79.475000000000009 79.375 76.975000000000009
76.575000000000003 76.475000000000009
75.975000000000009 75.875 75.77500000000
0006 74.975000000000009 74.674999999999997
74.174999999999997 73.775000000000006
73.174999999999997 72.875 72.375
72.174999999999997 72.575000000000003
72.775000000000006 73.174999999999997
73.475000000000009 74.375 74.775000000000006
75.375 76.174999999999997 76.975000000000009
77.174999999999997 77.975000000000009
77.674999999999997 77.975000000000009
77.674999999999997 78.275000000000006
78.275000000000006 77.775000000000006
77.674999999999997
Sheet pH1 glucose
secondsGlucose0107.0755109.77510107.67515102.5752099.975
2597.2753095.9753593.4754091.2754591.0755089.1755587.775
6086.6756585.1757084.5757582.7758081.6758579.7759078.875
9578.27510077.975rate corrected:0.0098-0.0018use the modular
value, ignore the sign
10)(0.008*60*0.237*3)/(100*10)12081.3750.00034128umol/mi
n/mg12580.87513080.37513579.77514079.17514578.77515078.
47515578.27516078.17516578.27517078.47517578.37518078.8
7518579.17519078.77519579.47520079.37520576.97521076.57
521576.47522075.97522575.87523075.77523574.97524074.675
24574.17525073.77525573.17526072.87526572.37527072.1752

7572.57528072.77528573.17529073.47529574.37530074.77530
575.37531076.17531576.97532077.17532577.97533077.675335
77.97534077.67534578.27535078.27535577.77536077.675
Glucose 0 5 10 15 20 25 30 35 40 45 50
55 60 65 70 75 80 85 90 95 100 105
110 115 120 125 130 135 140 145 150 155 160
165 170 175 180 185 190 195 200 205 210 215
220 225 230 235 240 245 250 255 260 265 270
275 280 285 290 295 300 305 310 315 320 325
330 335 340 345 350 355 360 107.075
109.77500000000001 107.675 102.575
99.975000000000009 97.275000000000006
95.975000000000009 93.475000000000009
91.275000000000006 91.075000000000003
89.174999999999997 87.775000000000006
86.674999999999997 85.174999999999997
84.575000000000003 82.775000000000006
81.674999999999997 79.775000000000006 78.875
78.275000000000006 77.975000000000009
81.475000000000009 82.075000000000003
81.674999999999997 81.375 80.875 80.375
79.775000000000006 79.174999999999997
78.775000000000006 78.475000000000009
78.275000000000006 78.174999999999997
78.275000000000006 78.475000000000009 78.375
78.875 79.174999999999997 78.775000000000006
79.475000000000009 79.375 76.975000000000009
76.575000000000003 76.475000000000009
75.975000000000009 75.875 75.775000000000006
74.975000000000009 74.674999999999997
74.174999999999997 73.775000000000006
73.174999999999997 72.875 72.375
72.174999999999997 72.575000000000003
72.775000000000006 73.174999999999997
73.475000000000009 74.375 74.775000000000006
75.375 76.174999999999997 76.975000000000009

77.174999999999997 77.975000000000009
77.674999999999997 77.975000000000009
77.674999999999997 78.275000000000006
78.275000000000006 77.775000000000006
77.674999999999997 Glucose 15 20 25 30 35
40 45 50 55 60 65 70 75 80 85 90 95
100 105 110 115 120 125 102.575
99.975000000000009 97.275000000000006
95.975000000000009 93.475000000000009
91.275000000000006 91.075000000000003
89.174999999999997 87.775000000000006
86.674999999999997 85.174999999999997
84.575000000000003 82.775000000000006
81.674999999999997 79.775000000000006 78.875
78.275000000000006 77.975000000000009
81.475000000000009 82.075000000000003
81.674999999999997 81.375 80.875 150 155
160 165 170 175 180 185 190 195 200
78.475000000000009 78.275000000000006
78.174999999999997 78.275000000000006
78.475000000000009 78.375 78.875
79.174999999999997 78.775000000000006
79.475000000000009 79.375
pH5 galactosesecondsGlucoseGlucoseglucose average
stdev0109109.15109.0750.10606601725107.1107.25107.1750.1
06066017210103.4103.55103.4750.106066017215102.1102.251
02.1750.106066017220100.1100.25100.1750.10606601722597.3
97.4597.3750.10606601723095.895.9595.8750.10606601723593
.793.8593.7750.10606601724092.792.8592.7750.106066017245
9191.1591.0750.10606601725090.490.5590.4750.106066017255
89.389.4589.3750.10606601726087.187.2587.1750.1060660172
6586.286.3586.2750.1060660172708686.1586.0750.1060660172
7584.784.8584.7750.10606601728083.283.3583.2750.10606601
728582.782.8582.7750.10606601729081.581.6581.5750.106066
01729580.280.3580.2750.106066017210080.180.2580.1750.106
0660172glucose

added10572.973.0572.9750.106066017211072.772.8572.7750.1
06066017211572.672.7572.6750.106066017212072.372.4572.37
50.106066017212572.672.7572.6750.106066017213072.672.757
2.6750.106066017213572.472.5572.4750.106066017214072.572
.6572.5750.106066017214571.671.7571.6750.106066017215071
.471.5571.4750.106066017215571.371.4571.3750.10606601721
6071.471.5571.4750.106066017216571.171.2571.1750.1060660
17217071.371.4571.3750.106066017217571.571.6571.5750.106
066017218071.371.4571.3750.106066017218571.371.4571.3750
.106066017219071.271.3571.2750.106066017219571.171.2571.
1750.106066017220070.870.9570.8750.1060660172Azide
added20570.470.5570.4750.106066017221070.270.3570.2750.1
06066017221570.370.4570.3750.106066017222070.270.3570.27
50.106066017222570.570.6570.5750.106066017223070.570.657
0.5750.106066017223570.570.6570.5750.106066017224070.670
.7570.6750.106066017224570.470.5570.4750.106066017225070
.971.0570.9750.10606601722557171.1571.0750.1060660172260
72.472.5572.4750.106066017226571.972.0571.9750.106066017
227071.271.3571.2750.106066017227570.670.7570.6750.10606
6017228070.971.0570.9750.106066017228570.370.4570.3750.1
06066017229070.971.0570.9750.10606601722957171.1571.075
0.106066017230070.470.5570.4750.106066017230570.370.4570
.3750.106066017231071.471.5571.4750.106066017231571.571.
6571.5750.106066017232071.471.5571.4750.106066017232571.
771.8571.7750.106066017233071.571.6571.5750.106066017233
571.771.8571.7750.106066017234071.671.7571.6750.10606601
7234571.271.3571.2750.106066017235071.471.5571.4750.1060
66017235571.371.4571.3750.106066017236071.271.3571.2750.
1060660172
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.1060 6601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0. 10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0 5 10 15 20 25 30
35 40 45 50 55 60 65 70 75 80 85 90
95 100 105 110 115 120 125 130 135 140 145
150 155 160 165 170 175 180 185 190 195 200
205 210 215 220 225 230 235 240 245 250 255
260 265 270 275 280 285 290 295 300 305 310

315 320 325 330 335 340 345 350 355 360
109.075 107.175 103.47500000000001 102.175
100.1 75 97.375 95.875 93.775000000000006
92.775000000000006 91.075000000000003
90.475000000000009 89.375 87.174999999999997
86.275000000000006 86.075000000000003
84.775000000000006 83.275000000000006
82.775000000000006 81.575000000000003
80.275000000000006 80.174999999999997
72.975000000000009 72.775000000000006
72.674999999999997 72.375 72.674999999999997
72.674999999999997 72.475000000000009
72.575000000000003 71.674999999999997
71.475000000000009 71.375 71.475000000000009
71.174999999999997 71.375 71.575000000000003
71.375 71.375 71.275000000000006
71.174999999999997 70.875 70.475000000000009
70.275000000000006 70.375 70.275000000000006
70.575000000000003 70.575000000000003
70.575000000000003 70.674999999999997
70.475000000000009 70.975000000000009
71.075000000000003 72.475000000000009
71.975000000000009 71.275000000000006
70.674999999999997 70.975000000000009 70.375
70.975000000000009 71.075000000000003
70.475000000000009 70.375 71.475000000000009
71.575000000000003 71.475000000000009
71.775000000000006 71.575000000000003
71.775000000000006 71.674999999999997
71.275000000000006 71.475000000000009 71.375
71.275000000000006
Sheet pH5
galactosesecondsGlucose0109.0755107.17510103.47515102.175
20100.1752597.3753095.8753593.7754092.7754591.0755090.47
55589.3756087.1756586.2757086.0757584.7758083.2758582.77
59081.5759580.27510080.175rate corrected:0.0098-0.0018use

the modular value, ignore the sign
10)(0.008*60*0.237*3)/(100*10)12072.3750.00034128umol/mi
n/mg12572.67513072.67513572.47514072.57514571.67515071.
47515571.37516071.47516571.17517071.37517571.57518071.3
7518571.37519071.27519571.17520070.87520570.47521070.27
521570.37522070.27522570.57523070.57523570.57524070.675
24570.47525070.97525571.07526072.47526571.97527071.2752
7570.67528070.97528570.37529070.97529571.07530070.47530
570.37531071.47531571.57532071.47532571.77533071.575335
71.77534071.67534571.27535071.47535571.37536071.275
Glucose 0 5 10 15 20 25 30 35 40 45 50
55 60 65 70 75 80 85 90 95 100 105
110 115 120 125 130 135 140 145 150 155 160
165 170 175 180 185 190 195 200 205 210 215
220 225 230 235 240 245 250 255 260 265 270
275 280 285 290 295 300 305 310 315 320 325
330 335 340 345 350 355 360 109.075 107.175
103.47500000000001 102.175 100.175 97.375
95.875 93.775000000000006 92.775000000000006
91.075000000000003 90.475000000000009 89.375
87.174999999999997 86.275000000000006
86.075000000000003 84.775000000000006
83.275000000000006 82.775000000000006
81.575000000000003 80.275000000000006
80.174999999999997 72.975000000000009
72.775000000000006 72.674999999999997 72.375
72.674999999999997 72.674999999999997
72.475000000000009 72.575000000000003
71.674999999999997 71.475000000000009 71.375
71.475000000000009 71.174999999999997 71.375
71.575000000000003 71.375 71.375
71.275000000000006 71.174999999999997 70.875
70.475000000000009 70.275000000000006 70.375
70.275000000000006 70.575000000000003

70.575000000000003 70.575000000000003
70.674999999999997 70.475000000000009
70.975000000000009 71.075000000000003
72.475000000000009 71.975000000000009
71.275000000000006 70.674999999999997
70.975000000000009 70.375 70.975000000000009
71.075000000000003 70.475000000000009 70.375
71.475000000000009 71.575000000000003
71.475000000000009 71.775000000000006
71.575000000000003 71.775000000000006
71.674999999999997 71.275000000000006
71.475000000000009 71.375 71.275000000000006
Glucose 15 20 25 30 35 40 45 50 55 60
65 70 75 80 85 90 95 100 105 110 115
120 125 102.175 100.175 97.375 95.875
93.775000000000006 92.775000000000006
91.075000000000003 90.475000000000009 89.375
87.174999999999997 86.275000000000006
86.075000000000003 84.775000000000006
83.275000000000006 82.775000000000006
81.575000000000003 80.275000000000006
80.174999999999997 72.975000000000009
72.775000000000006 72.674999999999997 72.375
72.674999999999997 150 155 160 165 170 175
180 185 190 195 200 71.475000000000009 71.375
71.475000000000009 71.174999999999997 71.375
71.575000000000003 71.375 71.375
71.275000000000006 71.174999999999997 70.875
pH9 galactosesecondsGlucoseGlucoseglucose average
stdev0112112.15112.0750.10606601725111.9112.05111.9750.1
06066017210108.9109.05108.9750.106066017215104104.15104
.0750.106066017220101101.15101.0750.10606601722598.798.8
598.7750.10606601723096.396.4596.3750.10606601723594.294
.3594.2750.10606601724091.691.7591.6750.10606601724589.5
89.6589.5750.10606601725088.488.5588.4750.10606601725586

.386.4586.3750.10606601726084.284.3584.2750.106066017265
83.383.4583.3750.10606601727082.282.3582.2750.1060660172
7579.980.0579.9750.1060660172807979.1579.0750.1060660172
8578.278.3578.2750.10606601729077.277.3577.2750.10606601
729576.376.4576.3750.10606601721007575.1575.0750.1060660
172glucose
added10570.971.0570.9750.106066017211069.469.5569.4750.1
06066017211569.269.3569.2750.106066017212068.768.8568.77
50.106066017212568.969.0568.9750.106066017213068.168.256
8.1750.106066017213567.968.0567.9750.106066017214067.867
.9567.8750.106066017214567.367.4567.3750.106066017215066
.967.0566.9750.106066017215566.666.7566.6750.10606601721
6066.166.2566.1750.106066017216565.966.0565.9750.1060660
17217065.665.7565.6750.106066017217565.365.4565.3750.106
066017218064.965.0564.9750.106066017218564.564.6564.5750
.106066017219064.364.4564.3750.106066017219564.164.2564.
1750.106066017220063.964.0563.9750.1060660172Azide
added20574.975.0574.9750.106066017221079.980.0579.9750.1
06066017221581.281.3581.2750.106066017222083.883.9583.87
50.106066017222583.283.3583.2750.106066017223082.983.058
2.9750.106066017223582.382.4582.3750.106066017224082.182
.2582.1750.106066017224580.180.2580.1750.106066017225079
.479.5579.4750.106066017225578.578.6578.5750.10606601722
6077.177.2577.1750.106066017226576.676.7576.6750.1060660
17227075.375.4575.3750.106066017227574.874.9574.8750.106
066017228073.773.8573.7750.106066017228572.572.6572.5750
.106066017229072.272.3572.2750.106066017229571.871.9571.
8750.106066017230070.270.3570.2750.106066017230570.170.2
570.1750.106066017231069.369.4569.3750.106066017231569.2
69.3569.2750.106066017232068.368.4568.3750.1060660172325
68.168.2568.1750.106066017233067.267.3567.2750.106066017
233566.967.0566.9750.106066017234066.566.6566.5750.10606
6017234565.765.8565.7750.106066017235065.165.2565.1750.1
06066017235564.364.4564.3750.10606601723606464.1564.075
0.1060660172

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.1060 6601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798113
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0. 10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798113 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615

0.10606601717798615 0 5 10 15 20 25 30
35 40 45 50 55 60 65 70 75 80 85 90
95 100 105 110 115 120 125 130 135 140 145
150 155 160 165 170 175 180 185 190 195 200
205 210 215 220 225 230 235 240 245 250 255
260 265 270 275 280 285 290 295 300 305 310
315 320 325 330 335 340 345 350 355 360
112.075 111.97500000000001 108.97500000000001
104.075 101.075 98.775000000000006 96.375
94.275000000000006 91.674999999999997
89.575000000000003 88.475000000000009 86.375
84.275000000000006 83.375 82.275000000000006
79.975000000000009 79.075000000000003
78.275000000000006 77.275000000000006 76.375
75.075000000000003 70.975000000000009
69.475000000000009 69.275000000000006
68.775000000000006 68.975000000000009
68.174999999999997 67.975000000000009 67.875
67.375 66.975000000000009 66.674999999999997
66.174999999999997 65.975000000000009
65.674999999999997 65.375 64.975000000000009
64.575000000000003 64.375 64.174999999999997
63.974999999999994 74.975000000000009
79.975000000000009 81.275000000000006 83.875
83.275000000000006 82.975000000000009 82.375
82.174999999999997 80.174999999999997
79.475000000000009 78.575000000000003
77.174999999999997 76.674999999999997 75.375
74.875 73.775000000000006 72.575000000000003
72.275000000000006 71.875 70.275000000000006
70.174999999999997 69.375 69.275000000000006
68.375 68.174999999999997 67.275000000000006
66.975000000000009 66.575000000000003
65.775000000000006 65.174999999999997 64.375
64.075000000000003
Sheet pH9 galactose

secondsGlucose0112.0755111.97510108.97515104.07520101.07
52598.7753096.3753594.2754091.6754589.5755088.4755586.37
56084.2756583.3757082.2757579.9758079.0758578.2759077.27
10)(0.008*60*0.237*3)/(100*10)12068.7750.00034128umol/mi
n/mg12568.97513068.17513567.97514067.87514567.37515066.
97515566.67516066.17516565.97517065.67517565.37518064.9
7518564.57519064.37519564.17520063.97520574.97521079.97
521581.27522083.87522583.27523082.97523582.37524082.175
24580.17525079.47525578.57526077.17526576.67527075.3752
7574.87528073.77528572.57529072.27529571.87530070.27530
570.17531069.37531569.27532068.37532568.17533067.275335
66.97534066.57534565.77535065.17535564.37536064.075
Glucose 0 5 10 15 20 25 30 35 40 45 50
55 60 65 70 75 80 85 90 95 100 105
110 115 120 125 130 135 140 145 150 155 160
165 170 175 180 185 190 195 200 205 210 215
220 225 230 235 240 245 250 255 260 265 270
275 280 285 290 295 300 305 310 315 320 325
330 335 340 345 350 355 360 112.075
111.97500000000001 108.975000000000 01
104.075 101.075 98.775000000000006 96.375
94.275000000000006 91.674999999999997
89.575000000000003 88.475000000000009 86.375
84.275000000000006 83.375 82.275000000000006
79.975000000000009 79.075000000000003
78.275000000000006 77.275000000000006 76.375
75.075000000000003 70.975000000000009
69.475000000000009 69.275000000000006
68.775000000000006 68.975000000000009
68.174999999999997 67.975000000000009 67.875
67.375 66.975000000000009 66.674999999999997
66.174999999999997 65.975000000000009

65.674999999999997 65.375 64.975000000000009
64.575000000000003 64.375 64.174999999999997
63.974999999999994 74.975000000000009
79.975000000000009 81.275000000000006 83.875
83.27500 0000000006 82.975000000000009 82.375
82.174999999999997 80.174999999999997
79.475000000000009 78.575000000000003
77.174999999999997 76.674999999999997 75.375
74.875 73.775000000000006 72.575000000000003
72.275000000000006 71.875 70.275000000000006
70.174999999999997 69.375 69.275000000000006
68.375 68.174999999999997 67.275000000000006
66.975000000000009 66.575000000000003
65.775000000000006 65.174999999999997 64.375
64.075000000000003 Glucose 15 20 25 30 35
40 45 50 55 60 65 70 75 80 85 90 95
100 105 110 115 120 125 104.075 101.075
98.775000000000006 96.375 94.275000000000006
91.674999999999997 89.575000000000003
88.475000000000009 86.375 84.275000000000006
83.375 82.275000000000006 79.975000000000009
79.075000000000003 78.275000000000006
77.275000000000006 76.375 75.075000000000003
70.975000000000009 69.475000000000009
69.275000000000006 68.775000000000006
68.975000000000009 150 155 160 165 170 175
180 185 190 195 200 66.975000000000009
66.674999999999997 66.174999999999997
65.97500000000000 9 65.674999999999997 65.375
64.975000000000009 64.575000000000003 64.375
64.174999999999997 63.974999999999994
pH1 galactose secondsGlucoseGlucoseglucose average
stdev0107107.15107.0750.10606601725104.5104.65104.5750.1
06066017210102102.15102.0750.10606601721599.299.3599.27
50.10606601722095.996.0595.9750.10606601722593.893.9593.
8750.10606601723091.992.0591.9750.10606601723589.990.058

9.9750.10606601724088.688.7588.6750.10606601724586.987.0
586.9750.10606601725085.885.9585.8750.10606601725583.984
.0583.9750.10606601726082.782.8582.7750.1060660172658181
.1581.0750.10606601727080.380.4580.3750.10606601727579.1
79.2579.1750.10606601728078.778.8578.7750.10606601728577
.277.3577.2750.10606601729076.977.0576.9750.106066017295
75.175.2575.1750.10606601721007575.1575.0750.1060660172g
lucose
added10576.676.7576.6750.106066017211076.376.4576.3750.1
06066017211576.576.6576.5750.106066017212076.876.9576.87
50.106066017212576.376.4576.3750.106066017213076.776.857
6.7750.106066017213576.476.5576.4750.106066017214076.276
.3576.2750.106066017214576.176.2576.1750.106066017215076
76.1576.0750.106066017215575.976.0575.9750.1060660172160
75.875.9575.8750.106066017216575.675.7575.6750.106066017
217075.275.3575.2750.106066017217574.975.0574.9750.10606
6017218074.874.9574.8750.106066017218574.674.7574.6750.1
06066017219074.174.2574.1750.106066017219573.673.7573.67
50.106066017220073.573.6573.5750.1060660172Azide
added20570.870.9570.8750.106066017221070.270.3570.2750.1
06066017221569.769.8569.7750.106066017222069.869.9569.87
50.106066017222570.570.6570.5750.10606601722307070.1570.
0750.106066017223570.170.2570.1750.106066017224069.369.4
569.3750.106066017224570.170.2570.1750.106066017225070.8
70.9570.8750.106066017225570.270.3570.2750.1060660172260
70.971.0570.9750.106066017226571.471.5571.4750.106066017
227071.571.6571.5750.106066017227570.870.9570.8750.10606
6017228071.972.0571.9750.106066017228570.870.9570.8750.1
06066017229072.172.2572.1750.106066017229571.471.5571.47
50.106066017230072.272.3572.2750.106066017230571.871.957
1.8750.106066017231071.972.0571.9750.106066017231571.871
.9571.8750.106066017232071.671.7571.6750.106066017232570
.870.9570.8750.106066017233070.971.0570.9750.10606601723
35703703.15703.0750.106066017234070.470.5570.4750.106066
017234570.270.3570.2750.106066017235070.470.5570.4750.10
6066017235570.370.4570.3750.106066017236093.4493.5993.51

50.1060660172
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10 606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717796604 0.10606601717798615

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798 615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717796604

0.10606601717798615 0.10606601717798615
0.10606601717798615 0.10606601717798615
0.10606601717798615 0 5 10 15 20 25 30
35 40 45 50 55 60 65 70 75 80 85 90
95 100 105 110 115 120 125 130 135 140 145
150 155 160 165 170 175 180 185 190 195 200
205 210 215 220 225 230 235 240 245 250 255
260 265 270 275 280 285 290 295 300 305 310
315 320 325 330 335 340 345 350 355 360
107.075 104.575 102.075 99.275000000000006
95.975000000000009 93.875 91.975000000000009
89.975000000000009 88.674999999999997
86.975000000000009 85.875 83.975000000000009
82.775000000000006 81.075000000000003 80.375
79.174999999999997 78.775000000000006
77.275000000000006 76.975000000000009
75.174999999999997 75.075000000000003
76.674999999999997 76.375 76.575000000000003
76.875 76.375 76.775000000000006
76.475000000000009 76.275000000000006
76.174999999999997 76.075000000000003
75.975000000000009 75.875 75.674999999999997
75.275000000000006 74.975000000000009 74.875
74.674999999999997 74.174999999999997
73.674999999999997 73.575000000000003 70.875
70.275000000000006 69.775000000000006 69.875
70.575000000000003 70.075000000000003
70.174999999999997 69.375 70.174999999999997
70.875 70.275000000000006 70.975000000000009
71.475000000000009 71.575000000000003 70.875
71.975000000000009 70.875 72.174999999999997
71.475000000000009 72.275000000000006 71.875
71.975000000000009 71.875 71.674999999999997
70.875 70.975000000000009 703.07500000000005
70.475000000000009 70.275000000000006
70.475000000000009 70.375 93.515000000000001

Sheet pH1 galactose
secondsGlucose0107.0755104.57510102.0751599.2752095.9752
593.8753091.9753589.9754088.6754586.9755085.8755583.9756
082.7756581.0757080.3757579.1758078.7758577.2759076.9759
575.17510075.075rate corrected:0.0098-0.0018use the modular
value, ignore the sign
10)(0.008*60*0.237*3)/(100*10)12076.8750.00034128umol/mi
n/mg12576.37513076.77513576.47514076.27514576.17515076.
07515575.97516075.87516575.67517075.27517574.97518074.8
7518574.67519074.17519573.67520073.57520570.87521070.27
521569.77522069.87522570.57523070.07523570.17524069.375
24570.17525070.87525570.27526070.97526571.47527071.5752
7570.87528071.97528570.87529072.17529571.47530072.27530
571.87531071.97531571.87532071.67532570.87533070.975335
703.07534070.47534570.27535070.47535570.37536093.515
Glucose 0 5 10 15 20 25 30 35 40 45 50
55 60 65 70 75 80 85 90 95 100 105
110 115 120 125 130 135 140 145 150 155 160
165 170 175 180 185 190 195 200 205 210 215
220 225 230 235 240 245 250 255 260 265 270
275 280 285 290 295 300 305 310 315 320 325
330 335 340 345 350 355 360 107.075 104.575
102.075 99.275000000000006 95.975000000000009
93.875 91.975000000000009 89.975000000000009
88.674999999999997 86.975000000000009 85.875
83.975000000000009 82.775000000000006
81.075000000000003 80.375 79.174999999999997
78.775000000000006 77.275000000000006
76.975000000000009 75.174999999999997
75.075000000000003 76.674999999999997 76.375
76.575000000000003 76.875 76.375
76.775000000000006 76.475000000000009
76.275000000000006 76.174999999999997

76.075000000000003 75.975000000000009 75.875
75.674999999999997 75.275000000000006
74.975000000000009 74.875 74.674999999999997
74.174999999999997 73.674999999999997
73.575000000000003 70.875 70.275000000000006
69.775000000000006 69.875 70.575000000000003
70.075000000000003 70.174999999999997 69.375
70.174999999999997 70.875 70.275000000000006
70.975000000000009 71.475000000000009
71.575000000000003 70.875 71.975000000000009
70.875 72.174999999999997 71.475000000000009
72.275000000000006 71.875 71.975000000000009
71.875 71.674999999999997 70.875
70.975000000000009 703.07500000000005
70.475000000000009 70.275000000000006
70.475000000000009 70.375 93.515000000000001
Glucose 15 20 25 30 35 40 45 50 55 60
65 70 75 80 85 90 95 100 105 110 115
120 125 99.275000000000006 95.975000000000009
93.875 91.975000000000009 89.975000000000009
88.674999999999997 86.975000000000009 85.875
83.975000000000009 82.775000000000006
81.075000000000003 80.375 79.174999999999997
78.775000000000006 77.275000000000006
76.975000000000009 75.174999999999997
75.075000000000003 76.674999999999997 76.375
76.575000000000003 76.875 76.375 150 155
160 165 170 175 180 185 190 195 200
76.075000000000003 75.975000000000009 75.875
75.674999999999997 75.275000000000006
74.975000000000009 74.875 74.674999999999997
74.174999999999997 73.674999999999997
73.575000000000003

Evidence-Based Practice (EBP) Instructions for Scenario 1. Begin.docx

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