Expt 3
- 1. Objectives : a) To analyse the kidney’s function in maintaining acid-base balance. b) To identify the certain normal constituents of urine. c) To study the principle of Multistix and Weyl Test. Materials and Methods : Refer to Laboratory Manual, Universiti Tunku Abdul Rahman page 63-64 Results : 1) Ammonium Tube contains Observation Urine Clear solution and pungent smell evolved after boiling. NH3 Clear solution and pungent smell evolved after boling. 2) Creatinine (Weyl test) Test Observation Urine Creatinine Sodium Nitroprusside Color changed to darker Color changed to light yellow from light yellow. red from colorless. Sodium Hydroxide Color changed to light Colored changed to orange. darker red. Glacial Acetic Acid Color changed to light Colored changed to light yellow. yellow. 3) Uric Acid Test Observation Urine Sodium Urate Folin Reagent- Color changed from Color changed from Phosphotungstic acid yellow to dark blue. The colorless to dark blue. pH is 7 or 8. The pH is 12. 4) Glucose (Benedict’s Test)