Foldscope DBT Victus Labs
- 1. Department of Biotechnology Ministry of Science & Technology Govt. of India Victus Laboratories India Private Limited Coimbatore, Tamil Nadu, India Funding Agency Powered by
- 2. Principal Investigator Dr. K.R. Arunkumar Technical Director Victus Laboratories India Private Limited Ramanathapuram, Coimbatore. arunvictus@gmail.com Junior Research Fellow Dr. A. Manikandan Victus Laboratories India Private Limited Ramanathapuram, Coimbatore. maniaraa1@gmail.com
- 3. Dr. Manikandan A Victus Lab Profile Dr. Manikandan has completed Ph.D at 2018 in Dept. of Botany, Bharathidasan University. He has experience and specialization in plant-microbes interaction, control the crop disease and induced defense responses using polymer based nanoparticles. He had published his research findings in a peer reviewed journals with high impact factor. He has experience in the field of modern biotechnology. He is currently working in the foldscope research for plant disease diagnosis kit.
- 4. What’saboutfoldscope… o It is an origami-based optical microscope unique invention by an Indian Scientist Dr. Manu Prakash, Stanford University, USA. o The foldscope is an ultra affordable, minimalist microscope made from folded paper and a lens. o It’s price is less than a dollar. o The lens that provides 140X magnification. o It’s a weighs less and small enough to fit in a pocket. o The foldscope has been instrumental in studying fungi, cataloging pollen, examining parasites, and analyzing bacteria etc. o It’s a portable diagnostic alternative for conventional microscope. Why Microscope Go for Foldscope
- 5. Objectives… To survey the plant diseases from fields and check the morphological characters of plant pathogen and beneficial microbes using by foldscope. To develop smart phone based rapid and cheap diagnostics for plant diseases. Methodology… Disease infected plant samples were collected from local fields for the screening of microbes. Infected tissues were scraped with dissection needle on the glass slide containing drop of sterile distilled water. After to make the cover slip on the glass slides. Each prepared slides were examined using by foldscope (Code: 0002C6E80FBD). The images were captured by the help smart phone (Nokia 6.1) by focusing slide under the lens of foldscope with 140X Magnification power and using LED illuminator as a source of light. Foldscope attach with smart phone
- 6. Plant pathogens screened using by foldscope… Morphological identification of Pestalotiopsis spp. Conidia shape was fusiform. Conidia of Pestalotiopsis spp. Results… Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 7. Helminthosporium spp… Mycelium Conidia Conidia on the conidiophores Conidiophore Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 8. Curvularia spp… Conidia Mycelium Conidia on the conidiophores Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 9. Alternaria spp… Mycelium Conidia Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 10. Puccinia spp… Conidia Conidium Infected Sandlum album leaf were focused by foldscope Erysiphe spp… Conidium Conidia Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 11. Pythium spp… Pythium spp.oospores Fusarium spp… Macroconidia Macro and micro conidia Macroconidia Micro conidia Chlamydo spore Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 12. Pyricularia spp… Infected Oryza sativa leaf were focused by foldscope Conidium Conidium Macrophomina spp… Mycelium PycnidiumPycnidium Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 13. Penicillum spp… Conidia Conidia on the conidiophores Conidia on the conidiophores Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 14. Beneficial microbes screened using by foldscope… Trichoderma spp… Conidia Chlamydospore Chlamydospore Chlamydospore Conidia Paecilomyces spp… Conidia on the conidiophores Conidia Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 15. Metarhizium spp… Aspergillus spp… Conidia ConidiaMyceliumConidia on the conidiophores Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 16. Verticillium spp… ConidiaMycelium Conidiophore Beauveria spp… Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 17. Tea pests and its symptoms screened using by foldscope… Tea Mosquito Bug Helopeltis theivora Pink Mite Acaphylla theae Symptoms Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 18. Purple Mite Calacarus carinatus Symptoms Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 19. Red Spider Mite Oligonychus coffeae Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 20. Alternaria Leaf Blight Cercospora Leaf Spot Symptoms Photo credits Manikandan /DBT Foldscope Project, Victus Lab Alternaria conidia Carrot diseases and its symptoms screened using by foldscope …
- 21. Late Blight/Septoria Spot Bacterial Blight Symptoms Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 22. Nitrogen Deficiency Magnesium Deficiency Cutworm Agrotis sppSymptoms Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 23. Potato Early BlightPotato Early Blight Alternaria spp Photo credits Manikandan /DBT Foldscope Project, Victus Lab Potato diseases and its symptoms screened using by foldscope…
- 24. Potato Late Blight Sulfur DeficiencyMagnesium Deficiency Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 25. Whiteflies Potassium Deficiency Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 26. Cladosporium leaf blotchCladosporium leaf blotch Cladosporium conidia Photo credits Manikandan /DBT Foldscope Project, Victus Lab Garlic diseases and its symptoms screened using by foldscope …
- 27. Botrytis leaf blight White tip Calcium Deficiency Photo credits Manikandan /DBT Foldscope Project, Victus Lab
- 28. Conclusion… Considering the images captured, foldscope is an useful tool for characterization of microorganisms and helpful in plant diseases diagnosis . Future Perspective… Our future aim is to develop a mobile app targeted towards farmers and create a database of plant symptom images for foldscope assisted plant disease diagnostics.