Grant defence.ppt
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This document proposes a study to characterize muscarinic acetylcholine receptors (mAchR) in neuroblastoma cells. Neuroblastoma is a cancer that affects the nervous system in children. The study aims to examine the role of mAchR in neuroblastoma progression and its interaction with other oncogenic pathways. It also seeks to assess mAchR levels in different grades of neuroblastoma samples. The results could reveal new therapeutic targets and strategies for treating high-risk neuroblastoma, which currently has a low cure rate.
Grant defence.ppt
- 1. Functional Characterization of Muscarinic Acetylcholine Receptors (mAchR) on Neuroblastoma cells Name: Arijit Bhowmik Roll no. : 65 Course work batch: 2012-13 GRANT DEFENCE
- 2. What is neuroblastoma? image adapted from O'Neill: Principles of Pediatric Surgery. © 2003, Elsevier.
- 3. Why we need to target Neuroblastoma ?
- 4. Type of Neuroblastoma Image taken from http://bmi.osu.edu/cialab/neuro_project.php
- 5. Pathways associated with Neuroblastoma progression EGFR, IL6, VEGF, NGF, Notch, STAT-1 , Sonic hedgehog signaling Along with these upregulation of N-Myc in this type of cancer is very common
- 6. Synaptogenesis V. Vetri, R. Carrotta, P. Picone, M. Di Carlo, V. Militello. Biochim. Biophys. Acta - Protein & Proteomics 1804, (2010) 173-183.
- 7. Presence of mAchR in neuronal cells
- 8. mAchR is a GPCR receptor • Amanita muscaria, the mushroom from which muscarine was isolated. • These receptors are sensitive to this compound. Thus it is known as Muscarinic Acetylcholine Receptors (mAchR)
- 9. G protein-coupled receptors (GPCR) comprise the largest family of drug targets The 2012 Nobel prize in chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was "crucial for understanding how G-protein–coupled receptors function. Brian Kobilka Robert Lefkowitz
- 11. Muscarinic acetylcholine receptor pathway Drug News Perspect 2004, 17(4): 258
- 12. GPCR receptors have crosstalk with the major oncogenic signaling pathways So in our proposal we have predicted that Muscarinic Acetylcholine Receptors (mAchR) and its related pathway could be a possible mediator of oncogenic crosstalks in neuroblastoma
- 13. The study will mainly focus on…… Quantitative in situ assessment of mAchR in different grades of neuroblastoma samples. Role of mAchR in progressive neuroblastoma occurrence and its effect on proliferation or apoptosis. The possible existence of cross talk of mAchR and its pathway members with other key signaling molecules affecting the occurrence of malignant neuroblastomas.
- 14. In Summary……. Neuroblastoma is a disease exhibiting extreme heterogeneity. Therapy for high-risk neuroblastoma the past two decades resulted in cures only about 20% of the pateint population. Targeting mAchR in neuroblastoma may generate new avenues in molecular biology and therapeutic strategies. Thus novel drugs will be inducted into clinical practice guidelines of high grade neuroblastoma treatment.