High content imaging with the yokogawa cv7000 system
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The Yokogawa Cell Voyager 7000 is a high content imaging platform that features a dual spinning disk confocal system for high quality live cell imaging. It can image up to three samples simultaneously using sCMOS cameras and offers a variety of objectives and laser choices. The system is automation-ready and can image standard plate types from 96 to 1536 wells as well as microscope slides. It provides high throughput imaging with exceptional image quality and reproducibility.
High content imaging with the yokogawa cv7000 system
- 1. Yokogawa Cell Voyager 7000 Wako Automation Introduction and Overview
- 3. Wako Pure Chemicals Japan, USA, Germany ! Wako Diagnostics Japan, USA, Germany ! Wako Automation USA
- 4. High Content Imaging Platform Yokogawa CV7000
- 5. High Content Imaging Platform Automation-Ready
- 6. Yokogawa CSU-W1 •Minimum Bleaching and Toxicity •Reduced Crosstalk •Visualize Dynamic Events in Live Cells Dual Spinning Disk Systems
- 7. 4X Cells Imaged Reduced Crosstalk Thicker Samples CSU-W1- Wide View Dual Spinning Disk
- 8. Live Cell Imaging HeLa cell with Azami green, GFP, 10X, 10% 488nm laser 3 days
- 9. Yokogawa CSU-X1 Dual Spinning Disk Live Cell Imaging Live Cells imaged every 20 minutes for 24 hours
- 11. Simultaneous Imaging with Three (3) Cameras sCMOS -20˚C 5.5MP, 2560 x 2160 6.5µm pixel
- 13. Choose Up To 6 Lenses Dry: 2x, 4x NA .16, 10x NA .4, 20x NA .75, 40x NA .95, 20x NA .45 LWD ! Water Immersion: 40xNA 1.15, 60x NA 1.2 Phase Contrast: 10X, 20X
- 14. Lens Field of View 2x 8.3 mm x7mm 4x 4.15 mm x 3.5 mm 10x 1.66 mm x1.4 mm 20x 0.83 mm x0.7 mm 40x 0.415 mm x0.35 mm 60x 0.276 mm x0.23 mm sCMOS Camera FOV
- 15. Plate Type Well Size Lens 96 well 6.5mm 2x objective 384 well 3.25mm 4x objective 1536 well 1.5mm 10x objective sCMOS Camera FOV
- 16. HeLa cell mitochondria stained with MitoTracker® Red CMXRos 0.2 um steps, 21 slices, 60X water immersion lens.
- 17. Live Cell Imaging ! 40X lens, Laser; 561 nm, Laser power; 10%, Exposure time; 20ms, 3 days, 20 min. Neural Progenitor Cells
- 18. Reproducibility of stage Plate removed 6X Imaged with 60x Water
- 20. Lasers: Choice of Four 405, 488, 532, 561, 640 ! UV LED: 365 (not confocal) Halogen Lamp (not confocal)
- 21. Neurite Imaging 60X W lens, NA 1.2
- 23. 6 to 1536 well plates Microscope Slides ! Incubated Stage 35˚C - 40˚C, CO2, Humidity Live Cell Imaging ! Laser Based Autofocus Image Based Autofocus ! Dispense Option single channel for kinetics assays
- 24. Image Standard Plate Types & Microscope Slides
- 25. ! ! ! 3 colors, 96 wells, <2 min 3 colors, 384 wells, <5 min 3 colors, 1536 wells, <18 min (50ms, 1 field, 1 z Stack) Imaging Speed (3 cameras) Exceptional Image Quality
- 27. Thick Sample Imaging Possible with CSU-W1
- 28. Cy2 Collagen IV (Basement membrane) - green CY3 Pgp9.5 (pan-neuronal Marker)- red DAPI DNA (Cell nucleuc)- Blue Exp： 200ms, Z axis 80um , 1.0µm spacing, 81 slices in total Objective: 60X
- 30. Laser light focused through microlens Reduced photo-‐bleaching and photo-‐toxicity Be:er confocal images Yokogawa Dual Spinning Disk System
- 31. unmatched speed 4x field of view live cell imaging for days