IDNADEX: Improving DNA Data Exchange Validation Studies of a Global STR System
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The document outlines a proposal for improving DNA data exchange through a validation study of a 21 STR typing system designed for forensic analysis. It discusses the performance of various STR markers, PCR amplification techniques, and the analytical thresholds for accurate DNA profiling. The research aims to enhance the reliability of DNA mixtures and improve global data sharing capabilities in forensic genetics.
![Body fluid mixture samples from GHEP & GEDNAP proficiency exercises
DNA SAMPLES BODY FLUIDS &
CONTRIBUTORS
[DNA]
ng/ul
DNAMIX01-L1 Total: 5.7
Male: 0.8
DNAMIX01-L2 Total: 0.7
Male: 0.4
DNAMIX01-L3 Total: 0.7
Male: 0.6
DNAMIX02-L1 Total: 13
Male: 15
DNAMIX03 Total: 4
Male: 2
DNAMIX04 Total: 13
Male: 8
DNAMIX05 Total: 9
Male: 3
DNAMIX06 Total: 1.7
Male: 1
DNAMIX07 Total: 3.3
Male: 1.8
DNAMIX08 Total: 1.9
Male: 1.8
DNAMIX09 Total: 1.7
Male: 1.3
DNAMIX10 Total: 3
Male: 0
SEMENSALIVA BLOOD
TwoContributors
ThreeContributors
DNA
SAMPLES
BODY FLUIDS &
CONTRIBUTORS
[DNA]
ng/ul
DNAMIX11 Total: 7
Male: 3
DNAMIX12 Total: 1.6
Male: 1.7
DNAMIX13 Total: 3.5
Male: 1.7
DNAMIX14 Total: 1.7
Male: 0.8
http://www.gednap.de
http://www.gep-isfg.org
Allele Concordance with other kits
MNC](https://image.slidesharecdn.com/2-2co014210madridhidsalonsoetalfinalmarch2015-150804202645-lva1-app6892/85/IDNADEX-Improving-DNA-Data-Exchange-Validation-Studies-of-a-Global-STR-System-16-320.jpg)
IDNADEX: Improving DNA Data Exchange Validation Studies of a Global STR System
- 1. IDNADEX Improving DNA Data Exchange Validation Studies of a Global STR System INTCF-Madrid SERVICIO DE BIOLOGIA Supported by a grant from the ISEC programe Madrid 04/03/2015
- 2. ESS STR Core Loci CODIS STR Core Loci Other STR Loci In Forensic Kits Spanish Database (200.000 profiles) STR Markers in ESS typing kits CSF1PO CSF1PO TPOX TPOX D5S818 D5S818 D7S820 D7S820 D13S317 D13S317 D16S539 D16S539 D16S539 FGA FGA FGA FGA TH01 TH01 TH01 TH01 VWA VWA VWA VWA D3S1358 D3S1358 D3S1358 D3S1358 D8S1179 D8S1179 D8S1179 D8S1179 D18S51 D18S51 D18S51 D18S51 D21S11 D21S11 D21S11 D21S11 D22S1045 D22S1045 D1S1656 D1S1656 D10S1248 D10S1248 D2S441 D2S441 D12S391 D12S391 D2S1338 D2S1338 D2S1338 D19S433 D19S433 D19S433 SE33 SE33 Penta D Penta E Proposed 21 STR Typing System CSF1PO TPOX D5S818 D7S820 D13S317 D16S539 FGA TH01 VWA D3S1358 D8S1179 D18S51 D21S11 D22S1045 D1S1656 D10S1248 D2S441 D12S391 D2S1338 D19S433 SE33 Proposal: Validation of a single 21 STR typing system based on 6-Dye CE detection to improve DNA data Exchange locally and globally ISEC Programme Targeted Call 2011 Awarded July 2012 Requirements to the Companies 21 STR covering ESS & CODIS + D2, D19 & SE33 Concordance of primer sequences design with previous systems Master mix with high tolerance to PCR inhibitor for casework application
- 3. Validation Studies on DNA Mixtures Using a 6-Dye System Containing 23 STR and 3 Y-STR Loci Pablo Martín, Lourdes Fernández de Simón, Gracia Luque, Mª José Farfán, Antonio Alonso Validation Studies on DNA Mixtures Using the PowerPlex® Fusion 6C System Promega Webinar Series. Genetic Identity Original Webinar Date: Thursday, February 5, 2015 Pablo Martín, Lourdes Fernández de Simón, Gracia Luque, Mª José Farfán, Antonio Alonso IDNADEX: Improving DNA Data Exchange FSI Genetics 13 (2014) 68-78
- 4. Marker Chromosomal Location Basic Repeat motif Dye Label Allele sizes (bp) He DP CE D3S1358 3p21.31 TCTA/TCTG 6-FAM™ 96-141 0.7993 0.9207 0.6930 vWA 12p13.31 TCTA/TCTG 156-209 0.7817 0.9405 0.4253 D16S539 16q24.1 GATA 227-267 0.7324 0.9061 0.5179 CSF1PO 5q33.3-34 AGAT 282-318 0.7465 0.8754 0.4550 TPOX 2p23-2per AATG 337-377 0.7183 0.8397 0.3189 D8S1179 8q24.13 TCTA/TCTG VIC® 114-171 0.8310 0.9384 0.6578 D21S11 21q11.2-q21 TCTA/TCTG 182-239 0.8451 0.9512 0.6930 D18S51 18q21.33 AGAA 261-342 0.8732 0.9664 0.6930 D2S441 2p14 TCTA NED™ 76-113 0.7641 0.9035 0.5510 D19S433 19q12 AAGG 118-171 0.8345 0.9178 0.6562 TH01 11p15.5 AATG/ATG 179-218 0.7958 0.9179 0.5179 FGA 4q28 CTTT 223-378 0.8697 0.9624 0.7685 D22S104 22q12.3 ATT TAZ™ 88-121 0.6549 0.7782 0.5510 D5S818 5q21-31 AGAT 138-183 0.6901 0.8658 0.3969 D13S317 13q22-31 TATC 149-243 0.7746 0.9253 0.6930 D7S820 7q11.21-22 GATA 262-298 0.7676 0.9397 0.4859 SE33 6q14 Complex AAAG 307-428 0.9577 0.9790 0.8458 D10S1248 10q26.3 GGAA SID™ 85-129 0.7500 0.9078 0.5179 D1S1656 1q42.2 TAGA 160-207 0.8944 0.9626 0.7685 D12S391 12p13.2 AGAT/AGAC 216-268 0.8838 0.9619 0.7305 D2S1338 2q35 TGCC/TTCC 281-349 0.8732 0.9629 0.6202 General characteristics of autosomal STR markers included in the Globalfiler Kit and forensic biostatistical parameters in the Spanish population (N=284) Garcia et al. Forensic Sci Int Genet. 6(2) (2012) Additional Sex markers of Globalfiler System Y indel Yq11.221 VIC 81-86 bp Amelogenin X: p22.1-22.3 ; Y: p11.2 98-104 bp DYS391 Yq11.21 364-389 bp Multiplex STR System Probability of Identity Probability of Paternity Exclusion NGMSElect 2.5 X 10 -21 99.999998% Globalfiler 3.1 X 10 -26 99.99999995%
- 5. Validation Parameters Type & Number of Samples Analyzed Analytical Threshold 22 PCR negative controls Sensitivity & Stochastic Threshold 3 Human genomic DNA samples of known human DNA concentration (SRM-2372A, DNA Control 007 & 1 genomic DNA sample from an anonymous male donor) (84 samples: Quadruplicate analysis of 7 dilution series) Precision and Accuracy 24 injections of the Globalfiler allelic ladder 90 human genomic DNA samples Repeatability & reproducibility 3 Human genomic DNA samples of known STR profile (30 samples: Quintuplicate analysis of 5 replicates by two operators) Heterozygous balance 85 Human genomic DNA samples Stutter Threshold 85 Human genomic DNA samples Genotype concordance 80 Human genomic DNA samples from saliva swabs of anonymous donors 10 genomic DNA samples of the DNA profiling standard SRM-2391b DNA mixtures 1 male /female DNA mixture in 7 different ratios (1 ng of total DNA) 1 male /female DNA mixture in 7 different ratios (3 ng of total DNA) (42 samples: Triplicate analysis of 7 dilution series) 15 DNA mixture samples from proficiency exercises Species specificity 21 genomic DNA from human–associated microbial species PCR reduced reaction volume 10 human genomic DNA samples of known STR profile Stability study in Case-Type Samples 54 forensic DNA samples previously analyzed with different STR Kits Validation Parameters & Materials
- 6. Methods Forensic samplesReference samples DNA ExtractionDNA Extraction Real time PCR Quantifiler® Duo Kit Targets: RPPH1 , SRY & IPC DNA QuantificationDNA Quantification 1 X 95ºC for 60 s 29 X 94ºC for 10 s 59ºC for 90 s 1X 60ºC for 10 min PCR AmplificationPCR Amplification Sample Injection: 15 s at 1.2kv CE: 1550 s at 13 kv CE Detection 3500 Genetic AnalyzerCE Detection 3500 Genetic Analyzer GlobalFiler® User guide Peak Amplitude 50 & 100 RFU Data AnalysisData Analysis Management of samples, analysis and results Comparison & Concordance studies
- 7. GlobalFiler® kit profile from a reference saliva swab (DNA input: 500 pg; PCR Reaction volume :12.5 ul) Performance of PCR (peak height) under reduced reaction volume (12,5 µl) in comparison with the standard PCR reaction volume (25 µl) from a set of 10 human genomic DNA from saliva reference samples.
- 8. Blue Green Yellow Red Purple Average RFU 5,35 10,94 5,71 8,68 9,72 Standard deviation 2,67 3,61 2,30 3,01 3,19 Max. RFU 50 48 53 45 47 Min. RFU 1 2 1 2 2 Average RFU + 3 Standard deviation 13,35 21,77 12,61 17,71 19,28 Average RFU + 10 Standard deviation 32,04 47,02 28,73 38,79 41,58 2 X (Ymax - Ymin) 98 92 104 86 90 Range of analysis: 70-450 bpRange of analysis: 70-450 bp Analytical Threshold : 22 PCR negative controls AT: 100 RFUAT: 100 RFU AT: 50 RFUAT: 50 RFU
- 9. Sensitivity study & stochastic threshold: Heat maps summarizing the sensitivity results of the last five DNA serial dilutions (from 250 to 15.25 pg) 250-1 250-2 250-3 250-4 125-1 125-2 125-3 230 125-4 62.5-1 62.5-2 161 138 530 62.5-3 62.5-4 281 186 205 31.25-1 149 103 278 375 130 127 31.25-2 183 353 142 112 194 599 214 157 31.25-3 111 233 335 146 126 201 305 765 31.25-4 168 223 203 212 916 193 15.5-1 168 117 179 203 129 187 114 230 237 133 135 120 15.5-2 171 136 187 266 290 107 162 210 148 15.5-3 114 168 228 132 126 113 15.5-4 286 173 279 123 119 113 103 160 274 811 313 542 228 110 202 120 342 191 225 190 264 166 143 102 194 321 102 117 139 239 105 127 267 122 149 400 170 127 205 278 139 508 281 190 163 314 180 257 135 104 118 194 226 145 172 249 136 169 214 114 114 222 148 237 156 115 120 175 181 154 143 254 135 142 196 162 661 158 102 165 121 121 176 172 342 476 354 166 101 170 622 198 174 154 393 144 110 207 147 113 113 150 440 152 394 226 424 131 131 212 184 158 553 158 2372 A Control 007 Genomic DNA D 3 S 1 3 5 8 v W A D 1 6 S 5 3 9 C S F 1 P O T P O X Y - I N D E L A M E L D 8 S 1 1 7 9 D 2 1 S 1 1 D 1 8 S 5 1 D Y S 3 9 1 D 2 S 4 4 1 D 1 9 S 4 3 3 T H 0 1 F G A D 2 2 S 1 0 4 5 D 5 S 8 1 8 D 1 3 S 3 1 7 D 7 S 8 2 0 S E 3 3 D 1 0 S 1 2 4 8 D 1 S 1 6 5 6 D 1 2 S 3 9 1 D 2 S 1 3 3 8 D 3 S 1 3 5 8 v W A D 1 6 S 5 3 9 C S F 1 P O T P O X Y - I N D E L A M E L D 8 S 1 1 7 9 D 2 1 S 1 1 D 1 8 S 5 1 D Y S 3 9 1 D 2 S 4 4 1 D 1 9 S 4 3 3 T H 0 1 F G A D 2 2 S 1 0 4 5 D 5 S 8 1 8 D 1 3 S 3 1 7 D 7 S 8 2 0 S E 3 3 D 1 0 S 1 2 4 8 D 1 S 1 6 5 6 D 1 2 S 3 9 1 D 2 S 1 3 3 8 D 3 S 1 3 5 8 v W A D 1 6 S 5 3 9 C S F 1 P O T P O X Y - I N D E L A M E L D 8 S 1 1 7 9 D 2 1 S 1 1 D 1 8 S 5 1 D Y S 3 9 1 D 2 S 4 4 1 D 1 9 S 4 3 3 T H 0 1 F G A D 2 2 S 1 0 4 5 D 5 S 8 1 8 D 1 3 S 3 1 7 D 7 S 8 2 0 S E 3 3 D 1 0 S 1 2 4 8 D 1 S 1 6 5 6 D 1 2 S 3 9 1 D 2 S 1 3 3 8 100RFU100RFU 250-1 250-2 250-3 250-4 125-1 125-2 125-3 230 125-4 62.5-1 62.5-2 161 530 62.5-3 62.5-4 205 31.25-1 149 56 130 127 31.25-2 183 353 599 157 31.25-3 51 233 126 765 66 31.25-4 223 81 86 212 916 193 15.5-1 179 203 129 81 187 94 53 114 230 135 120 15.5-2 114 266 162 210 86 62 15.5-3 114 55 168 132 126 113 55 95 15.5-4 173 279 123 119 103 92 160 274 110 202 54 342 191 190 72 90 166 65 60 194 55 92 84 89 77 117 91 105 72 127 74 122 75 85 400 86 170 76 127 62 205 278 60 85 63 508 190 82 163 314 180 257 118 226 145 54 172 249 136 87 114 53 222 148 53 115 120 86 83 52 175 181 55 154 59 50 143 57 71 196 59 51 102 121 121 170 154 83 113 113 150 152 394 60 226 88 97 158 553 158 58 2372 A Control 007 Genomic DNA 50 RFU50 RFU Stochastic threshold RFU value of the highest surviving false homozygous peak when allele drop-out is observed at heterozygous markers: 916 RFU for D12S391 at 31.25 pg of DNA input
- 10. 12,84 2,77 0 0 0 30,9 19,09 1,04 0,34 0 56,25 78,12 98,95 99,65 100 0 20 40 60 80 100 15,5 31,25 62,5 125 250 29,51 14,24 0 0 0 33,33 20,83 4,86 0,34 0 37,15 67,01 94,44 99,65 100 0 20 40 60 80 100 15,5 31,25 62,5 125 250 100RFU100RFU 50 RFU50 RFU Overall percentage results (SRM-2372A, DNA Control 007, and the genomic DNA from an anonymous donor) of full allele profiles , allele drop-out , and locus drop-out
- 11. Sensitivity study: Incidence of Drop-in at Low DNA input D2S441-007-15 PGD2S441-007-15 PG 69 RFU 69 RFU D7S820-007-62 PGD7S820-007-62 PG 113 RFU 113 RFU D22S104-007-62 PGD22S104-007-62 PG 66 RFU 66 RFU TPOX-007-62 PGTPOX-007-62 PG 84 RFU 84 RFU D21S11-007-125 PGD21S11-007-125 PG 84 RFU 84 RFU D3S1358-2372A-125 PGD3S1358-2372A-125 PG 63 RFU 63 RFU D8S1179-2372A-125 PGD8S1179-2372A-125 PG 100 RFU 100 RFU D2S441-2372A-250 PGD2S441-2372A-250 PG 59 RFU 59 RFU SE33-2372A-250 PGSE33-2372A-250 PG 191 RFU 191 RFU D8S1179-DONOR-31 PGD8S1179-DONOR-31 PG 103 RFU 103 RFU TH01-DONOR-62 PGTH01-DONOR-62 PG 80 RFU 80 RFU SE33-DONOR-250 PGSE33-DONOR-250 PG 155 RFU 155 RFU Detected Incidence of Drop-in over a threshold of 100 RFU (1,764 Determinations) 0.28 % Detected Incidence of Drop-in over a threshold of 50 RFU (1,764 Determinations) 0.68 %
- 12. Species specificity against genomic DNA samples from several human–associated microbial species. (Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus agalactiae (serogroup B), Staphylococcus aureus, Escherichia coli, Bordetella pertussis, Bordetella parapertussis, Alcaligenes faecalis, Acinetobacter calcoaceticus, Flavobacterium odoratum, Morganella morganii, Pseudomonas stutzeri, Providencia stuartii, Proteus mirabilis, Citrobacter freundii, Pseudomonas aeruginosa, Salmonella enteritidis, Neisseria lactamica, Shigella flexneri, and Serratia marcencens)
- 13. 500:500 - 1 500:500 - 2 500:500 - 3 333:666 - 1 333:666 - 2 333:666 - 3 166:833 - 1 166:833 - 2 166:833 - 3 111:888 - 1 111:888 - 2 111:888 - 3 91:909 - 1 91:909 - 2 91:909 - 3 62:938 - 1 62:938 - 2 62:938 - 3 48:952- 1 48:952 - 2 48:952 - 3 1:1 1:2 1:5 1:8 1.10 1:15 1:20 D 3 S 1 3 5 8 v W A D 1 6 S 5 3 9 C S F 1 P O T P O X I N D E L A M G D 8 S 1 1 7 9 D 2 1 S 1 1 D 1 8 S 5 1 D Y S 3 9 1 D 2 S 4 4 1 D 1 9 S 4 3 3 T H 0 1 F G A D 2 2 S 1 0 4 5 D 5 S 8 1 8 D 1 3 S 3 1 7 D 7 S 8 2 0 S E 3 3 D 1 0 S 1 2 4 8 D 1 S 1 6 5 6 D 1 2 S 3 9 1 D 2 S 1 3 3 8 TOTAL DNA INPUT: 1 ng TOTAL DNA INPUT: 1 ng 1500:1500 - 1 1500:1500 - 2 1500:1500 - 3 999:1998 - 1 999:1998 - 2 999:1998 - 3 498:2499 - 1 498:2499 - 2 498:2499 - 3 333:2664 - 1 333:2664 - 2 333:2664 - 3 273:2727 - 1 273:2727 - 2 273:2727 - 3 186:2814 - 1 186:2814 - 2 186:2814 - 3 144:2856 - 1 144:2856 - 2 144:2856 - 3 1:20 1:15 1.10 1:8 1:2 1:1 1:5 TOTAL DNA INPUT: 3 ng TOTAL DNA INPUT: 3 ng DNA mixtures series: Relative ratios of 1:1, 2:1, 5:1, 8:1, 10:1, 15:1, and 20:1 of female / male
- 14. Full profile of the minor male contributor: Series of 1 ng of total DNA at 5:1 mixture ratio DNA input of the minor male contributor: 166 pgDNA input of the minor male contributor: 166 pg
- 15. Full profile of the minor male contributor: Series of 3 ng of total DNA at 10:1 mixture ratio DNA input of the minor male contributor: 273 pgDNA input of the minor male contributor: 273 pg
- 16. Body fluid mixture samples from GHEP & GEDNAP proficiency exercises DNA SAMPLES BODY FLUIDS & CONTRIBUTORS [DNA] ng/ul DNAMIX01-L1 Total: 5.7 Male: 0.8 DNAMIX01-L2 Total: 0.7 Male: 0.4 DNAMIX01-L3 Total: 0.7 Male: 0.6 DNAMIX02-L1 Total: 13 Male: 15 DNAMIX03 Total: 4 Male: 2 DNAMIX04 Total: 13 Male: 8 DNAMIX05 Total: 9 Male: 3 DNAMIX06 Total: 1.7 Male: 1 DNAMIX07 Total: 3.3 Male: 1.8 DNAMIX08 Total: 1.9 Male: 1.8 DNAMIX09 Total: 1.7 Male: 1.3 DNAMIX10 Total: 3 Male: 0 SEMENSALIVA BLOOD TwoContributors ThreeContributors DNA SAMPLES BODY FLUIDS & CONTRIBUTORS [DNA] ng/ul DNAMIX11 Total: 7 Male: 3 DNAMIX12 Total: 1.6 Male: 1.7 DNAMIX13 Total: 3.5 Male: 1.7 DNAMIX14 Total: 1.7 Male: 0.8 http://www.gednap.de http://www.gep-isfg.org Allele Concordance with other kits MNC
- 17. Allele Concordance with other Kits (Intra & Inter-lab comparison) TwoContributors DNA SAMPLES BODY FLUIDS & CONTRIBUTORS Allele Concordance (Globalfiler, Identifiler , NGMSE, ESX17,…) DNAMIX01-L1 OK Partial DNA profile from the minor male contributor at a 7:1 DNA ratio DNAMIX01-L2 OK DNAMIX01-L3 OK DNAMIX02 OK DNAMIX03 OK Non-concordance at SE33 among different Kits (primer designs) DNAMIX04 OK DNAMIX05 OK Allele 28 at D12S391 (Out of ladder) DNAMIX06 OK DNAMIX07 OK DNAMIX08 OK DNAMIX09 OK DNAMIX10 OK (Identifiler, NGMSe, PPESX17, Fusion 6C, …)
- 18. Allele Concordance with other Kits (Intra & Inter-lab comparisons) ThreeContributors DNA SAMPLES BODY FLUIDS & CONTRIBUTORS Allele Concordance (Globalfiler, Identifiler , NGMSE, ESX17,…) DNAMIX11 OK CE Resolution of Microvariants at D12S391 DNAMIX12 OK CE Resolution of Microvariants at D12S391 DNAMIX13 OK DNAMIX14 OK (Identifiler, NGMSe, PPESX17, Fusion 6C, …)
- 19. Non-concordance at SE33 among different Kits (primer designs) Promega6CV2 SE33SE33 17 2117 21 28.2 32.228.2 32.2 GlobalFiler®kiit SE33SE33 17 2117 21 28.2 32.128.2 32.1
- 20. Allele 28 at D12S391 (Out of ladder) Promega6CV2 D12S391D12S391 D19S433D19S433 GlobalFiler®kit D12S391D12S391 D2S1338D2S1338
- 21. CE Resolution of Microvariants at D12S391 1616 17.3 18 18.3 19.3 GlobalFiler®kit D12S391D12S391 1616 20 17.3 18 18.3 19.3 Promega6CV2 D12S391D12S391 20.3 21 1515 16 20 D12S391D12S391 1515 16 20 21 D12S391D12S391 20.3 Automatic allele calling of 20.3 By GMIDX reanalysis with No Smoothing Automatic allele calling of 20.3 By GMIDX reanalysis with No Smoothing
- 22. Minimum Number of Contributors
- 23. Minimum Number of Contributors
- 24. Full GlobalFiler® profile example from a DNA extract with strong PCR inhibitors Sample: Epithelial cells in a glove QFDUO Total: 0 QFDUO Male: 0 IPC: Undetected Identifiler® kitIdentifiler® kitIdentifiler® kit GlobalFiler® kitGlobalFiler® kitGlobalFiler® kit Case-Type Samples: PCR INHIBITORS 3 DNA samples with PCR inhibitors showed full Globalfiler Profiles
- 25. Case-Type Samples: DNA DEGRADATION Degraded DNA obtained from 8 bone samples and 5 teeth samples (with a variable post-mortem interval from 3 month to 41 years) Sample: Pars Petrosa QFDUO Total: 0.0 25 QFDUO Male: 0 IPC: 29.7 Partial GlobalFiler® profile example from a 41 years old bone sample MODERATE DNA DEGRADATION
- 26. NGM SElect™ kitNGM SElect™ kit GlobalFiler® kitGlobalFiler® kit Sample: FFPE QFDUO Total: 0,0 4 QFDUO Male: 0.03 IPC: 30 Partial NGM SElect™ & GlobalFiler® profile examples from a severely degraded DNA sample Case- Type DNA Samples: HIGH DNA DEGRADATION FORMALIN FIXED BIOPSIES
- 27. Concluding Remarks The data reported demonstrates the GlobalFiler® PCR amplification system followed by CE detection in a 3500 Genetic Analyzer generates high quality, reproducible, precise, accurate, and sensitive profiling STR data, even from sub-nanogram amounts of genomic DNA template and also in the presence of powerful PCR inhibitors co-extracted with DNA from a high variety of forensic samples including the following DNA sources: blood, semen & saliva stains, hair, muscle, liver, bone and teeth. The GlobalFiler® system provides equivalent overall performance to previous forensic STR PCR kits, but with enhanced discrimination power for a better match efficiency that would reduce the chance of adventitious matches during DNA data exchange among national DNA databases. The use of GlobalFiler® kit would be also very beneficial to improve discrimination power of DNA analysis not only in criminal DNA databases, but also in many other forensic applications of autosomal STR profiling including missing person identification, disaster victim identification, mass grave investigations and kinship analysis.
- 28. PROJECT TEAM Pablo Martín Lourdes Fernández de Simón Gracia Luque Mª José Farfán INTCFINTCFINTCFINTCF----MadridMadridMadridMadrid SERVICIO DESERVICIO DESERVICIO DESERVICIO DE BIOLOGIABIOLOGIABIOLOGIABIOLOGIA BIOLOGY DEPARTMENT STAFF Acknowledgements Special thanks also to the staff of our microbiology laboratory for providing the microbial genomic DNA samples used in the specificity study. Oscar Garcia Biostatistical Calculation with the Spanish Population Data This project has been funded with support from the European Commission (grant HOME/2011/ISEC/AG/PRUM/4000002125 under the Prevention of and Fight against Crime (ISEC) programme of the European Commission-Directorate General Justice and Home Affairs). This publication reflects the views only of the authors, and the European Commission cannot be held responsible for any use which may be made of the information contained therein.
- 29. Speaker was provided travel and hotel support by Thermo Fisher Scientific for this presentation, but no remuneration. GlobalFiler® Express PCR Amplification system is For Forensic and Paternity Use Only. Applied Biosystems™ 3500 Series Genetic Analyzers are For Research, Forensic or Paternity Use Only. Not for use in diagnostic procedures. Life Technologies and its affiliates are not endorsing, recommending, or promoting any use or application of Life Technologies products presented by third parties during this seminar. Information and materials presented or provided by third parties are provided as-is and without warranty of any kind, including regarding intellectual property rights and reported results. Parties presenting images, text and material represent they have the rights to do so.