Introduction

Introduction

Air is a primary essential element for all living organisms; without air there is no life.
Many recent policy inventions have been undertaken to improve ambient air quality. But very
little is known about indoor air quality (IAQ). People spend majority of their time indoor mostly
in domestic environment, where their health may be affected mostly by indoor air pollution.

Air pollutants measured in the living rooms include Carbon monoxide, Carbon dioxide,
Nitrogen dioxide, Sulphur dioxide, velocity of air, humidity etc. Air quality refers to the nature
of conditioned air that circulates throughout the area where we work and live, that is the air we
breathe during most of our lives. IAQ refers not only to comfort, which is affected by
temperature and humidity but also to harmful biological contaminants and chemicals present in
the conditioned space. Indoor air quality may be broadly defined as the nature of air that affects
the health and well – being of occupants.

It refers to the physical, chemical, and biological characteristics of air in the indoor
environment within a home, building, or an institution or commercial facility. Indoor air
pollution is a concern in the developed countries, where energy efficiency improvements
sometimes make houses relatively airtight, reducing ventilation and raising pollutant levels.
Indoor air problems can be subtle and do not always produce easily recognized impacts on
health. Different conditions are responsible for indoor air pollution in the rural areas and the
urban areas.

In the developing countries, it is the rural areas that face the greatest threat from indoor
pollution, where some 3.5 billion people continue to rely on traditional fuels such as firewood,
charcoal, and cowdung for cooking and heating. Concentration of indoor pollutants in
households that burn traditional fuels are alarming. Burning such fuels produces large amount of
smoke and other air pollutants in the confined space of the home, resulting in high exposure.
Women and children are the groups most vulnerable as they spend more time indoors and are
exposed to the smoke. In 1992, the World Bank designated indoor air pollution in the developing
countries as one of the four most critical global environmental problems. Daily average of

1

pollutant level emitted indoors often exceed current WHO guidelines and acceptable levels.
Although hundreds of separate chemical agents have been identified in the smoke from biofuels,
the four most serious pollutants are particulates, carbon monoxide, polycyclic organic matter and
formaldehyde. Unfortunately, little monitoring has been done in rural and poor urban indoor
environment in a manner that is statistically rigorous.

In urban areas, exposure to indoor air pollution has increased due to a variety of reasons,
including the construction of more tightly sealed buildings, reduced ventilation, the use of
synthetic materials for building and furnishing and the use of chemical products, pesticides, and
household care products. Indoor air pollution can begin within the building or be drawn in from
outdoors. Other than nitrogen dioxide, carbon monoxide and lead, there are a number of other
pollutants that affect the air quality in an enclosed space.

Polyaromatic hydrocarbons
Polyaromatic hydrocarbons are potent atmospheric pollutants that consist of fused
aromatic rings and do not contain hetero atoms Napthelene is the simples example of a
PAH.They occurs due to incomplete combustion of fuels.eg:coal,tar and biomass.
They are also found in cooked foods.

List of Polyaromatic hydrocarbons
• Acenapthalene • Chrysene

• Acenapthene • Dibenzo(a,h) anthracene

• Anthracene • Fluoranthene

• Benzo(a) anthracene • Fluorene

• Benzo(a) fluoranthene • Indeno(123-cd) pyrene

• Benzo(a) pyrene • Napthalene

• Benzo(ghi) pyrene • Phenanthrene

• Benzo(k) fluoranthene • Pyrene

2

Factors affecting Indoor Air Quality (IAQ)
The factors affecting the IAQ mainly are the air conditioning system, indoor pollutants and
deterioration of outdoor air. Temperature, relative humidity, air velocity, amount of fresh air,
carbon dioxide, carbon monoxide, ozone, formaldehyde, benzene, respirable particles, volatile
organic compounds (VOC’s) and Radon are the particles affecting indoor air quality The
outdoor air usually enters through doors, windows and fresh air system. At present in the air
conditioning system, air filters trap the dust but it can’t purge harmful gas and germs.

Air pollution is a major environmental health problem affecting the developing and developed
countries. The concentration and duration of air contamination causes observable effects on
human health, animals, plants & materials. The greatest health impacts from air pollution
worldwide occur among the poorest and most vulnerable populations. It is a significant cause of
morbidity and mortality (Smith, 1993). In addition, they may be natural or man-made.

Pollutants can be classified as primary or secondary pollutants. The primary pollutants
are directly emitted from sources into the atmosphere, cause harm to people, monuments,
buildings etc. The following primary pollutants are also known to cause harm in high enough
concentrations:
• Carbon compounds, such as carbon monoxide, carbon dioxide, methane , and volatile
organic compounds.
• Nitrogen compounds, such as oxides of nitrogen, nitrous oxide, and ammonia.
• Sulphur compounds, such as hydrogen sulphide and sulphur dioxide.
• Halogen compounds, such as chlorides, fluoride and bromides
• Particulate Matter

Secondary pollutants are not directly emitted from sources but instead form in the
atmosphere from primary pollutants.
• Nitrous acid and nitric acid formed from oxides of nitrogen.
• Ozone (O3) formed from Sulphur dioxide and nitric acid droplets formed from nitrous
oxide.
• Sulphate and nitrate aerosols

3

Indoor Air Pollutants

The table shows some of the indoor air pollutants, there source and their potential health

effects.

Pollutant Indoor Sources Potential Health Effects

Polycyclic Aromatic Cooking, wood burning and other Cancer.
Hydrocarbons (PAHs) combustion .

Benzene and other organic Solvents, glues, cleaners, building Cancer, respiratory effects,
air toxics materials, moth repellents, dry- liver, kidney and brain
cleaned clothing, air fresheners. damage.

Particulate Matter Wood stoves, fireplaces, cooking, Lung cancer, cardiovascular
candles, aerosol sprays. effects, respiratory effects.

Sources of Indoor Air Pollution
Air pollutants are invisible and odorless. So detecting indoor air pollutants is difficult.
The four major sources of indoor air pollution are
• Combustion byproducts, such as smoke and carbon monoxide.
• Building materials, including carpets, wood products and paints.
• Household products and chemicals, such as cleaning solvents, adhesives and
Paint strippers.
• Biological contaminants such as molds, animal dander, germs, dust and mites.

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Impact of Indoor Air Pollutants
The health effects caused by air pollution may include difficulty in breathing, wheezing,
coughing and aggravation of existing respiratory and cardiac conditions. These effects can result
in increased medication use, increased doctor or emergency room visits, more hospital
admissions and premature death. The human health effects of poor air quality are far reaching,
but principally affect the body's respiratory system and the cardiovascular system. Individual
reactions to air pollutants depend on the type of pollutant a person is exposed to, the degree of
exposure, the individual's health status and genetics.

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STUDY AREA

BHADRAVATHI

Bhadravathi is an industrial town in Shivamogga District of Karnataka state, India. It is
situated at a distance of about 255 kilometres (158 mi) from the state capital, Bangalore and at
about 20 kilometres (12 mi) from the district headquarters, Shivamogga. Bhadravathi Taluk has
a total area of 675.08 square kilometres. Population of Bhadravathi according 2011 Census is
approximately 160,392. The latitude and longitude coordinates of Bhadravathi town are
13°50′24″N 75°42′07″E / 13.840°N 75.702°E / 13.840; 75.702. Bhadravathi is at an altitude of
Gonibeedu
597 mts (1,959 ft) above sea level.It derives the name from the Bhadra River which flows
through the city. It was earlier known as Benkipura.

The present study was conducted in different areas of Bhadhravathi. In several residences, the
residents still use the age old method of heating water by using firewood and coconut husk for
bathing which causes health problems in women and chidern.

6

Objectives of the Study

The main objective of the study is to assess the status of total suspended particulates and

Poly aromatic hydrocarbons in indoor environment of different houses in Bhadhravathi Industrial

Town.

Objectives
a) Assessment and evaluation of total suspended particulate matter in indoor environment of
different houses in Bhadhravathi Industrial Town.
b) Estimation of poly aromatic hydrocarbon level in the air borne particulates using High
power liquid chromatography.
c) To assess the health status of different families in Bhadhravathi Industrial Town.

7

Review of Literature

According to WHO report( 2002) indoor air is becoming more hazardous than outdoor air.
Indoor air pollution is responsible for 2.7% of the global burden of disease. Indoor smoke
ranks tenth as a risk factor of disease. But more than three billion people worldwide
continue to depend on solid fuels, including biomass fuels (wood, cow dung, crop waste,
agricultural residues) and coal for their energy needs (Bilkis et al., 2008).

In the World Health Report (2002) it was estimated that 1.6 million premature deaths are caused
annually by indoor air pollution. Certain population groups, like women and small children, are
vulnerable to the effects of indoor air pollution because their daily activities include exposure to
indoor air pollutants 90% of rural households in developing countries still rely on unprocessed
biomass fuels for most of the day. The amount of exposure in terms of the number of people,
exposure intensity and time is greater in developing world (Smith, 1993); approximately 76% of
all global particulate matter air pollution occurs indoors in the developing world.

Eye and respiratory symptoms have been attributed to indoor air pollutants in developing
countries (Ellegard, 1997; Ezzati and Kammen, 2001; Smith et al., 2000; Ellegard, 1996). Eye
irritation during cooking was studied in Lusaka, Maput and Hanoi in Zambia, Mozambique, and
Vietnam respectively; along with respiratory symptoms, it was found to be associated with
biomass use (Ellegard, 1997). The prevalence of respiratory symptoms was significantly
associated with the use of open fires in comparison to use of wood stoves with chimneys in
Guatemalan study (Bruce et al., 1998). A study from India observed the occurrence of
respiratory symptoms over six months and reported that a greater proportion of biomass users
suffered from respiratory symptoms with decreased pulmonary function compared to kerosene or
LPG users (Dutt et al., 1996).

Chronic bronchitis, an important public health problem of developing countries, has been shown
to be related to domestic biomass use in rural Bolivian villages (Albalak et al., 1999) and hill
region villages of Nepal (Pandey, 1989). The impact of such exposures is further aggravated by
poor ventilation combined with continuous and prolonged exposure to such sources, especially in
women and children (Bruce et al., 2002).

8

Household indoor air pollution concentration was measured during unvented burning (chimneys
blocked) and vented burning (chimneys open) of bituminous coal in Xuan Wei, China.
Concentration of particulate matter with an aerodynamic diameter of 10μm or less (PM10) and
of Benzo[a]pyrene(BaP) were measured in 43 homes during normal activities.

The use of chimneys led to a significant decrease in indoor air concentration of particulate matter
with an aerodynamic diameter of 10μm or less (PM10) by 66% and Benzo[a]pyrene(BaP) by
84% with the installation of the chimneys. The reduction of indoor pollution levels by the
installation of a chimney supports the epidemiology finding on the health benefits of stove
improvement. However, even in the presence of a chimney, the indoor air concentration for both
PM10 and BaP still exceeded the indoor air quality standards of China. (Linwei Tian et al.,
2009).

Polycyclic aromatic hydrocarbons (PAHs) belong to the group of persistent organic pollutants
(POPs). These are organic contaminants that are resistant to degradation, can remain in the
environment for long period and have the potential to cause adverse environmental effects.
Some of them are susceptible to dispersion on a global scale because, in addition to having
environmental persistence, they are "semi-volatile", i.e. under environmental conditions they
move between the atmosphere and the earth's surface in repeated, temperature-driven cycles of
deposition and volatilization (Wania and Mackay, 1996).

There are thousands of PAH compounds in the environment but in practice PAH analysis is
restricted to the determination of 6 to 16 compounds. Individual PAHs differ substantially in
their physical and chemical properties (Subramanium et al., 1994, Van Jaarsveld et al., 1997,
Maliszewska-Kordybach, 1998). PAHs are listed by the Unites States Environmental Protection
Agency (EPA) and the European Commission as priority pollutants.

PAHs emitted to the air can be transported over long distances before they are deposited with
atmospheric precipitation on soils, vegetation or sea and inland waters (Wild and Jones, 1995;
Van Jaarsveld et al., 1997). The presence of PAHs in all these elements of the environment may
create a risk not only to humans but also to all living organisms.

9

PAHs enter the atmosphere from the combustion and from volatilization. They are present in the
ambient air as vapours or adsorbed into airborne particulate matter (Subramanyam et al. 1994;
Van Jaarsveld et al., 1997; Wild and Jones, 1995).

10

Methodology
1. Measurement of total suspended particulate matter in air was done by gravimetric method
using Handy sampler (HDS-8).
2. Extraction of poly aromatic hydrocarbons by High performance liquid chromatography,
3. Assesment of health status of the residents through survey and questionnaires method.
Six areas comprising thirty residences were selected randomly for the study purpose
depending upon the use of biomass for heating and frequency of use.

Handy Sampler HDS-8

Handy sampler HDS-8 is a basic instrument used primarily for measuring concentration
of suspended time. These particulates usually range from 1 micron to 100 microns in size and
may be caused by a variety of process, such as incomplete combustion of solids, liquids or
gaseous fuels.

In the Handy sampler HDS-8, the air is passed through filter paper which is placed in
filter holder kept near sampling point. The flow rate capacity of air is 0-5 L.P.M per minute. It is
also used for the analysis of gases such as chloride, hydro chlorines, bromine, ammonia and
nitrous oxide. Impinger is used in which suitable solvent is filled. When the sampled air is passed
through, impinger the gases are dissolved in solvent. The solvent is then analyzed by appropriate
methods for respective gas.

Filter Paper
Collection of Airborne Suspended Particulate Matter:
The samples were collected in different houses of Bhadravathi industrial town. The
collected samples were transferred to clean polythene and analyzed in the laboratory as per
procedure.

Gravimetric method was used to determine the concentration of suspended particulate matter. A
known volume of air is drawn through a glass fiber filter of pore size 0.7 µm of known weight
and the filter paper is reweighed after sampling under controlled condition. This gives direct

11

measurement of particulate mass. During sampling, air flow rate is monitored through Rota
meter.

Glass fiber filter was exposed to the light source and inspected for pinhole and particles
of any other importation. Good and perfect filters are selected and weighed accurately in the
fourth decimal of a gram (in order to facilitate weighing it was necessary to hold the filter
loosely without producing any cramps over it) weight and serial number of the filter paper was
recorded on the field data sheet. The filter was unfolded after weighing and placed in plastic
folder for any man-handling. Before mounting the glass fibre filter paper on the sampler, the
sampler was carefully cleaned with a dust cloth. The sampler area of the equipment was properly
wiped out and kept clean before starting the sampler. The pre-weighed filter paper was placed in
the filter paper holder.

The glass fiber has two sides, rough surface and smooth surface, rough surface is the
reverse side of the filter. The sampler was switched on for its operation. The Rota meter level
(flow rate) was checked and recorded in the field data sheet. In the present study, samples were
collected over a 1-3 hours run. The rota meter reading and the corresponding flow rate and time
of closure data were recorded in the data sheet. The filter samples were dried in hot air oven
(1030-1050c) for an hour. After one hour the filter sample was cooled and to desiccated. The
filtered sample was weighed accurately by nearest to the fourth decimal of gram and recorded in
the data sheet. Replacing it back into the sample cover for further studies preserved the filter
paper.

Calculation:
Flow rate (v) = Flow rate in cubic meter per minute.
Period of sampling (T) = Total period of net running minutes
Volume of air sampled = Flow rate (V) x Sample period (T)
Weight of the dust (W) = Difference in final & initial weight of filter
Paper in grams
Concentration of dust = W x 106 microgram/Cu.m/V x T (mg/m3)

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Extraction of PAH’s:
High Performance Liquid Chromatography.
Polyaromatic hydrocarbons were identified and evaluated in different residences. They
were extracted from the filter paper collected from the pollution source.

The Glass fiber filter was used to determine the amount of particulate collected. After
collection, filters were wrapped with aluminum foil and stored at 4 0C in refrigerator until they
were sent to the laboratory for analysis by HPLC.

Results and Discussion

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Indoor air pollution caused by burning traditional fuels such as firewood, crop residue
and dung causes considerable damage to the health particularly,women and children. There is
evidence associating the use of biomass fuel with acute respiratory tract infection in children,
chronic obstructive lung disease and pneumoconiosis. The indoor air monitoring was carried out
in different residences in Bhadhravathi Industrialtal Town,Shimoga district using glass fiber
filter paper in Handy Sampler.

The Total Suspended Particulate Matter (TSPM) is collected and analyzed. The PAHs
were analyzed using High Pressure Liquid Chromatography (HPLC). The observation of Total
Suspended Particulate Matter (TSPM) is presented in Table 1. The average Total Suspended
Particulate Matter (TSPM) showed a wide variation ranging from 83 mg/m3to 9722 mg/m3 .
According to National Ambient Air Quality Standards (NAAQS) , it was found that the SPM
levels at all the monitored locations were higher than the prescribed limits in Indoors.

The high concentration of the suspended particulates in the residences may be due to the
use of mixed type of firewood and coconut husk. The incomplete combustion of firewood and
coconut husk emits suspended particulate matter, carbon monoxide, oxides of nitrogen, sulfur,
Polyorganicmaterial (POM), Polyaromatichydrocarbon (PAH), formaldehyde, etc (Bruce et al.,
2000).
The suspended particulate matter was analyzed for its concentration and identification of
various PAHs. The range of PAH concentration in different houses has been shown in table
Table.3.
It was found that PAHs concentration was maximum in house no-4 where 8 PAH were
detected. From the survey it was revealed that the economic condition of some houses is poor
and they use more quantity of bio fuels for heating purposes. The high concentration of PAH in
these houses as compared to others is due to insufficient ventilation in these houses.

Table 1- Sampling location, duration and total suspended particulate matter.

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Sl House no Location Samling Flow Total Initial Final Difference TSPM
no duration rate volume weight weight (g) in weight (µg/m3)
(minutes) LPM of air (g) (g)
LPM

15

1 House no 1 Jannapura 60 2 120 0.05896 0.05906 0.0001 833
3 House no 3 Jannapura 60 2 120 0.05789 0.05797 8.00E-03 667
6 House no 6 Hutha colony 60 2 120 0.05602 0.05607 5.00E-05 417
11 House no 11 Bandaralli 60 2 120 0.05935 0.05939 4.00E-05 333
14 House no 14 Bandaralli 60 2 120 0.05958 0.06 0.00042 3500
15 House no 15 Bandaralli 60 2 120 0.05822 0.05858 0.00036 3000
16 House no 16 Upper Hutha 60 2 120 0.05844 0.05949 5.00E-05 417
18 House no 18 Upper Hutha 60 2 120 0.0584 0.05854 0.00014 1167
20 House no 20 Upper Hutha 60 2 120 0.05758 0.05782 0.00024 2000
21 House no 21 Zincline 180 2 360 0.0588 0.0594 0.0006 1667
22 House no 22 Zincline 180 2 360 0.05832 0.0.5997 0.00165 4583
26 House no 26 Lower Hutha 180 2 360 0.05965 0.0601 0.00045 1250

TSPM= Total suspended particulate matter.

Table-2. Minimum, maximum, average and range of TSPM.

N TSPM (µg/m3)
Min Max Average Range
30 83 9722 1725 83-9722

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N = Number of houses
TSPM= Total suspended particulate matters.
Table 3: Minimum and Maximum concentration of PAHs Identified.

List of PAH Range in ng/m3

Acenapthalene BDL

Acenapthene 96-2031

Anthracene 6-171

Benzo(a) anthracene 2-56

Benzo(a) fluoranthene 56-6033

Benzo(a) pyrene 375-14913

Benzo(ghi) pyrene BDL

Benzo(k) fluoranthene 241-14898

Chrysene 1-15

Dibenzo(a,h) anthracene BDL

Fluoranthene 28-170

Fluorene BDL

Indeno(123-cd) pyrene BDL

Napthalene BDL

Phenanthrene 16-373

Pyrene 10-19

BDL=Below detection level

PAHs specific to biomass smoke emissions (as listed in Table 3 ) are analyzed. It included
volatile PAHs (acenaphthene), particle bound PAHs (chrysene, benzo(a)pyrene) plus PAHs
occurring in gas as well as in particle bound form (phenanthrene, anthracene, pyrene). The
observed PAHs exibit both carcinogenic and non-carcinogenic activity. Among the observed
PAH, Benzo[a]pyrene (BaP) is an important carcinogen (Miller et al., 1991). Anthracene is not a
carcinogen but methyl addition renders them to be a carcinogen.

17

The obtained values were much higher than the prescribed value of 1ng/m 3 (0.001 µg/m3) set by
WHO for ambient air PAH (WHO-Euro1987).

Table 4: P A H compounds tested from the collected air samples

Chemical Structural
Structural formula Chemical
compound formula
compound

18

Acenapthene Benzo(a)
anthracene

Anthracene Benzo[a]pyrene

Benzo(k)
Benzo(a) fluoranthene
fluoranthene

Phenanthrene
Chrysene

Pyrene
Fluoranthene

Summary and Conclusion
The present study summarizes the health risk in the indoor environment of several
residences In Bhadhravathi Industrial Town,Shimoga district, Karnataka. Evaluation of the air
borne contaminant such as, total suspended particulate matter and poly aromatic hydrocarbon
(PAHs) of suspended particles was carried out.

19

The concentration of the particulates was found to be higher than the permissible limit
and the particulates contain various type of PAHs such as , Acenapthene, Anthracene, Benzo (a)
anthracene, Benzo(a)fluoranthene, Benzo(a)pyrene, Benzo(k)fluoranthene, Chrysene,
Fluoranthene, Phenanthrene & Pyrene . Among the observed PAHs Benzo[a]pyrene is
carcenogenic in nature.

Indoor air pollution in India is responsible for a high degree of morbidity warranting
immediate steps for intervention. The first and the most important step in the prevention of
illness resulting from the use of biomass fuels is to educate the public, administrators and
politicians to ensure their commitment for the improvement of public health.

Indoor air pollution can be minimized by reducing combustion products in homes. The
intervention programme should include (I) public awareness; (ii) change in pattern of fuel use;
(iii) Using Solar water heaters (iv) Improvement in the ventilation. (v)Use modern heating
equipment which are non-pollutants.

Control Measures

 Improvement in ventilation
 Discourage smoking in your homes.
 Install and use exhaust fans that are vented to the outdoors in kitchens and bathrooms and
vent clothes dryers outdoors.

20

 Ventilate the attic and crawl spaces to prevent moisture build-up.
 If using cool mist or ultrasonic humidifiers, clean appliance according to manufacturer’s
instructions and refill with fresh water daily.
 Thoroughly clean and dry water-damaged carpets and building materials (within 24 hours
if possible) or consider removal and replacement.
 Keep the house clean. House dust mites, pollens, animal dander and other allergy-causing
agents can be reduced, although not eliminated, through regular cleaning.
 Take steps to minimize biological pollutants in basements.
 Take special precautions when operating fuel-burning unvented space heaters.
 Keep woodstove emissions to a minimum. Choose properly sized new stoves that are
certified as meeting EPA emission standards.
 Have central air handling systems, including furnaces, flues and chimneys, inspected
annually and promptly repair cracks or damaged parts.
 Throw away partially, full containers of old or unneeded chemicals safely.
 Exposure to benzene to a minimum.
 Exposure to perchloroethylene emissions from newly dry-cleaned materials to a
minimum.
 Unwanted pesticides disposed safely.
 Leave lead-based paint undisturbed if it is in good condition – do not sand or burn off
paint that may contain lead.
 Do not remove lead paint yourself.

Signature of Research Supervisor

Signature of Research Scholar

Bibliography

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Introduction

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Introduction