The document discusses isolation of the operative field in dentistry. Proper isolation through moisture control is important for operative procedures. Several components need to be controlled, including saliva, tongue and surrounding tissues. Rubber dam provides complete isolation and advantages for both patient and operator, such as a dry field. Other isolation materials include cotton rolls, throat shields and evacuators. Correct application and removal of the rubber dam is described. The goals of isolation are moisture control, retraction and prevention of harm during dental procedures.