This document discusses various isolation precautions used to prevent the spread of infectious diseases in healthcare settings. It describes the goals of isolation as protecting healthcare workers, other patients, visitors, and the environment from pathogens. The key components of isolation precautions are outlined, including standard precautions used for all patient care, and transmission-based precautions for airborne, droplet, and contact infections. Hand hygiene, personal protective equipment, patient placement, transport, cleaning, and other infection control measures are discussed for different isolation scenarios. Reversed isolation used to protect immunocompromised patients from environmental pathogens is also covered.