Krebs cycle.pptx
- 1. The institute of integrated & honors studies SUBMITTED TO:- SUBMITTED BY:- DR. SUHANA RAO AMIT KUMAR BOTANY DEPT.(IIHS,KUK) B.SC. MEDICAL (180009) BOTANY ASSIGNMENT ON Krebs Cycle 2020-2021
- 2. Krebs Cycle
- 3. Krebs Cycle Known as the Hans Krebs cycle.
- 4. Krebs Cycle Known as the Hans Krebs cycle. (After the scientist who discover it in1937.) Hans-Adolf-Krebs (Nobel -1953)
- 5. Krebs Cycle Known as the Hans Krebs cycle. (After the scientist who discover it in1937.) Also Citric acid cycle. (The molecule Citric acid/ citrate that play central role in cycle.) TCA Cycle (tricarboxylic acid cycle). Hans-Adolf-Krebs (Nobel -1953)
- 6. Krebs Cycle Krebs Cycle is series of chemical reaction that release stored energy of carbohydrate, fat and protein through the oxidation in the form of NADH and FADH.
- 7. Krebs Cycle Krebs Cycle is series of chemical reaction that release stored energy of carbohydrate, fat and protein through the oxidation in the form of NADH and FADH. Provide raw material like amino acid, NADH etc.
- 8. Site of occurrence- Eukaryotic Cell Prokaryotic Cell
- 9. Site of occurrence- Eukaryotic Cell Prokaryotic Cell Matrix of mitochondria
- 10. Site of occurrence- Eukaryotic Cell Prokaryotic Cell
- 11. Some background knowledge about the steps taken before the Krebs cycle-
- 12. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle
- 13. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA
- 14. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA where does it's come from?
- 15. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis
- 16. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C6H12O6 6C
- 17. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C6H12O6 6C 2 x Pyruvate (3C)
- 18. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C6H12O6 6C 2 x Pyruvate (3C) Pyruvate dehydrogenase complex COA
- 19. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C6H12O6 6C 2 x Pyruvate (3C) Pyruvate dehydrogenase complex COA (2C) CO2 & NADH
- 20. Some background knowledge about the steps taken before the Krebs cycle- Krebs cycle Acetyl CoA Glycolysis C6H12O6 6C 2 x Pyruvate (3C) Pyruvate dehydrogenase complex COA (2C) CO2 & NADH This shows that how Acetyl CoA is formed. Which is 2C compound and starting point of Krebs cycle.
- 21. Steps of Krebs cycle- Acetyl-CoA 2C
- 22. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C
- 23. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase
- 24. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate
- 25. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase 6C
- 26. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C
- 27. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase
- 28. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C
- 29. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA
- 30. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C
- 31. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C
- 32. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP
- 33. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP Fumarate 4C
- 34. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP Fumarate 4C FAD+H+ FADH Succinate dehydrogenase
- 35. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP Fumarate 4C FAD+H+ FADH Succinate dehydrogenase Malate 4C
- 36. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP Fumarate 4C FAD+H+ FADH Succinate dehydrogenase Malate 4C Fumarase
- 37. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP Fumarate 4C FAD+H+ FADH Succinate dehydrogenase Malate 4C Fumarase
- 38. Steps of Krebs cycle- Acetyl-CoA 2C Oxaloacetate 4C Citrate 6C Citrate Synthase Iso-citrate Aconitase α-ketoglutrate 6C Isocitrate dehydrogenase NAD+H+ NADH CO2 5C Succinyl CoA NAD+H+ NADH CO2 α-ketoglutrate dehydrogenase 4C Succinate 4C Succinyl CoA synthase GDP+ Pi GTP Fumarate 4C FAD+H+ FADH Succinate dehydrogenase Malate 4C Fumarase Malate dehydrogenase NAD+H+ NADH
- 39. Results- Summery Reaction of Krebs Cycle- Acetyl CoA + 3 NAD + FAD + GDP + PO4 + 2H2O 2CO2 + 3NADH + FADH + GTP + CoA
- 40. Results- Summery Reaction of Krebs Cycle- Acetyl CoA + 3 NAD + FAD + GDP + PO4 + 2H2O 2CO2 + 3NADH + FADH + GTP + CoA How many energy Produce? In one rotation – 12 ATP ( 3 NADH + 1 FADH + 1GTP) NADH = 3 ATP 1 FADH = 2 ATP 1GTP = 1ATP
- 41. Results- Summery Reaction of Krebs Cycle- Acetyl CoA + 3 NAD + FAD + GDP + PO4 + 2H2O 2CO2 + 3NADH + FADH + GTP + CoA How many energy Produce? In one rotation – 12 ATP ( 3 NADH + 1 FADH + 1GTP) NADH = 3 ATP 1 FADH = 2 ATP 1GTP = 1ATP For completion of reaction rotation should be twice So net ATP form - 12 ATP x 2 = 24 ATP
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