L4 key slides ferlin
- 2. Sperm function and sperm aneuploidies evaluated on single cells selected with high magnification technique from patients with severe testicular damage Single sperm cells Group A (n 100) (nuclear vacuole) Group B (n 100) (normal sperm) Mitochondria (%) 52.2+14.7* 13.3+4.9 Acridine Orange (%) 71.9+11.1* 5.3+3.0 TUNEL (%) 40.1+11.6* 9.3+4.8 Aneuploidies (%) 5.1+3.1 0.0 Garolla et al. High-power microscopy for selecting spermatozoa for ICSI by physiological status. RBM Online, 2008
- 4. Aitken et al. Int J Androl 2009
- 7. Problems with DNA fragmentation: What is to be measured and why? Different methods Standardization problems of the tests Intra-individual variability Normal values for fertility or normozoospermia? Type of sample analysed Small numbers Subgrouping of infertile men and IVF/ICSI cycles Identification of high-risk groups Effect of abstinence time
- 8. Influence of sperm DNA damage on IVF and ICSI Fertilization rate Negative effect Larson, 2000 Tomlinson, 2001 Tomsu, 2002 Benchaib, 2003 (ICSI) Larson, 2003 Henkel, 2004 Virro, 2004 Guerin, 2005 Borini, 2006 (ICSI) Boe-Hansen, 2006 (IVF) Avendano, 2009 Tarozzi, 2009 (ICSI) No effect Seli, 2004 Bungum, 2004 Gandini, 2004 Huang, 2005 Zini, 2005 Borini, 2006 (IVF) Boe-Hansen, 2006 (ICSI) Bungum, 2007 Lin, 2008 Nucopoullos, 2008 Velez de la Calle, 2008 Tarozzi, 2009 (IVF) Positive effect Payne, 2005 Pregnancy rate Negative effect Lopes, 1998 Host, 2000 (IVF) Morris, 2002 Benchaib, 2003 Payne, 2005 Huang, 2005 Velez de la Calle, 2008 No effect Tomlinson, 2001 Tomsu, 2002 Larson, 2003 Gandini, 2004 (ICSI) Henkel, 2004 Virro, 2004 Guerin, 2005 Zini, 2005 Lin, 2008 Avendano, 2009
- 9. HA-ICSI significantly improves embryo quality and development…but not implantation rate and pregnancy
- 10. New development Array CGH on single sperm