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Lab slides review
Simple Squamous Epithelial Tissue


                               Nucleus
Location:
Mesothelium
Endothelium

Function:
Filtration
Diffusion
Secretion
Stratified Squamous Epithelial




                          These keratinized epithelial cells will
                          Be sloughed off.

                                        Apical Layer




               Mitotic cells are rounder in appearance


Basal Layer
Simple (= single layer)
   Cuboidal
• Description: Single
  layer of cube-
  shaped cells.
• Location: small
  ducts of glands,
  kidney tubules,             Cell is as wide as it is high. One
                              central nucleus
  surface of ovary.
• Function: secretion
  & absorption
Simple Cuboidal
                                        Lumen:
                                        Inside space of
• Cells closely packed                  Tubular structure
  together and touching
  one another
• Has a basal surface      Nucleus
                                                 Apical
                                                 Surface
  and apical surface          Basal
                              Surface
• Avascular
• Sits on a basement
  membrane (difficult to
  see on slide)
Non- Ciliated Simple Columnar
• Description: Single
  layer of non-ciliated
  rectangular cells;
  contain goblet cells
  and sometimes
  microvilli.
• Location: GI tract,
  ducts, gallbladder
• Function: secretion
  and absorption
• Nuclei lie near base of cell
• Goblets secrete mucous at      Simple Columnar Cells
  apical surface to lubricate    Goblet Cell
  lining of GI, respiratory,                   Nucleus
  reproductive and urinary
  systems.
• Microvilli increase surface
  area for absorption.
            Goblet Cell
Pseudostratified Ciliated Columnar
                Epithelium
• Appears stratified
  because nuclei are at
  varying levels.
• Location:
  – Ciliated lines airways
    of upper respiratory
    tract.
• Function:
                             Goblet Cells
  – Mucus from Goblets
    traps foreign
    particles and are                Cilia
    swept away by cilia.
Goblet Cells secreting mucus

                                 Cilia




                               Nuclei at varying levels
Epithelial and
                         1
    Connective
• 1 – pseudostratified
  columnar epithelial    2

• 2- simple cuboidal
  epithelial             3


• 3- hyaline cartilage
  connective tissue
Collagen Fiber
                                          (thicker pink ones)
 Loose Areolar                          GROUND SUBSTANCE
Connective Tissue
                                               FIBER
                   MATRIX



           Fibroblast CELLS


                        Elastic Fiber
Teased Dense Regular Connective Tissue
• Forms tendons and
  ligaments and
  aponeuroses.
• Matrix looks white
  and shiny.
• Mostly collagen
  fibers with
  fibroblasts between
  rows.
• Strong attachment!
Teased Dense
  Regular CT
 (magnified)
               Fibroblasts




                             Fibers are
                             mostly
                             Collagen.
Reticular
Connective Tissue                       Reticular Cells

• Network of interlacing
  reticular fibers &           Blue staining
  reticular cells              Reticular fibers

• Found in stroma
  (framework) of liver,
  spleen, lymph nodes,
  red bone marrow
• Acts as a filter in spleen
  and lymph nodes.
Reticular Connective Tissue

• Remember that
  connective tissue is
  vascular. (Avascular
  epithelial tissue
  depends on diffusion
  of nutrients and
  waste through blood                       Blood vessel:
                                            Single layer of
  vessels in CT.                            Epithelial cells
                                            forms vessel tube.
Reticular        1
       CT                3
• 1- Reticular   2
  cells

• 2- Blood
  vessel

• 3- Reticular
  fibers
Hyaline
    Cartilage
• Often called gristle
• Bluish white smokey        chondrocytes
  looking ground
  substance
• Numerous chondrocytes
• Location: ends of long                    Ground S

  bones, ribs, nose,
  trachea, larynx, bronchi
• For flexibility and
  support
• Most abundant type of
  cartilage in body
Elastic Cartilage
• Chondrocytes are
  located within
  threadlike network
  of elastic fibers
• Location: Epiglottis
  of larynx, external
  ear, Eustachian
  tubes
• For support and
  shape
Elastic vs Hyaline Cartilage
Lacuna
(hollow space
holding chondrocyte)
                                     chondrocyte




                                                   Smoother
                                                   Appearance
                                                   Of ground
                       Elastic fibers throughout
                                                   substance
                       Ground substance
Adipose
        Tissue
• Adipocytes, “signet
  ring” cells with
  peripheral nuclei.
• For storage of
  triglycerides, to
  reduce heat loss, and
  serve as an energy                      nucleus
  reserve.
• Location: under skin,
  around heart &
  kidneys, yellow         “signet ring”
  marrow, behind           adipocyte

  eyeball
Osseous Tissue (Bone)
• Compact bone consists of
  osteons (haversion
  systems) that contain:
   –   Lamellae
   –   Lacuna
   –   Osteocytes
   –   Canaliculi
   –   Central (haversion)canals
• Spongy bone consists of
  thin plates called
  trabeculae filled with red
  marrow
Lamellae



 Central(Haversion)             Haversion
       Canal                    System



                      Lacunae
Lacunae (where osteocytes reside)




                                Canaliculi: small
                                Tunnels where osteocyte
                                processes run for diffusion
                                of nutrients and wastes
Skeletal Muscle
         Tissue
• Striated
    – Proteins
       • Actin
                              Striations
       • Myosin
•   Multinucleated
•   Voluntary
•   Attach to bones
•   For movement,
    communication, posture,
    heat production
Skeletal muscle fiber
with multiple nuclei
Lab slides review
Smooth (Visceral) Muscle Tissue
• No striations
• Spindle-shaped
• Single centrally located
  nucleus
• Nucleus is long and cigar
  shaped
• Involuntary
• Viscera
• Walls of hollow structures
   –   Blood vessels
   –   Airways to lungs
   –   Stomach
   –   Intestines
   –   Gallbladder
   –   Urinary bladder
Cigar shaped nuclei of smooth
muscle tissue
Smooth Muscle
Cardiac Muscle
        Tissue
• Striated, mono-
  nucleated, branching
• Self excitatory
• Intercalated discs       Intercalated disc

  attach fibers together
  and contain gap
  junctions (help with
                            nucleus
  conduction of
  electrical signals)
Name the tissues and structures you see.

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Lab slides review

  • 2. Simple Squamous Epithelial Tissue Nucleus Location: Mesothelium Endothelium Function: Filtration Diffusion Secretion
  • 3. Stratified Squamous Epithelial These keratinized epithelial cells will Be sloughed off. Apical Layer Mitotic cells are rounder in appearance Basal Layer
  • 4. Simple (= single layer) Cuboidal • Description: Single layer of cube- shaped cells. • Location: small ducts of glands, kidney tubules, Cell is as wide as it is high. One central nucleus surface of ovary. • Function: secretion & absorption
  • 5. Simple Cuboidal Lumen: Inside space of • Cells closely packed Tubular structure together and touching one another • Has a basal surface Nucleus Apical Surface and apical surface Basal Surface • Avascular • Sits on a basement membrane (difficult to see on slide)
  • 6. Non- Ciliated Simple Columnar • Description: Single layer of non-ciliated rectangular cells; contain goblet cells and sometimes microvilli. • Location: GI tract, ducts, gallbladder • Function: secretion and absorption
  • 7. • Nuclei lie near base of cell • Goblets secrete mucous at Simple Columnar Cells apical surface to lubricate Goblet Cell lining of GI, respiratory, Nucleus reproductive and urinary systems. • Microvilli increase surface area for absorption. Goblet Cell
  • 8. Pseudostratified Ciliated Columnar Epithelium • Appears stratified because nuclei are at varying levels. • Location: – Ciliated lines airways of upper respiratory tract. • Function: Goblet Cells – Mucus from Goblets traps foreign particles and are Cilia swept away by cilia.
  • 9. Goblet Cells secreting mucus Cilia Nuclei at varying levels
  • 10. Epithelial and 1 Connective • 1 – pseudostratified columnar epithelial 2 • 2- simple cuboidal epithelial 3 • 3- hyaline cartilage connective tissue
  • 11. Collagen Fiber (thicker pink ones) Loose Areolar GROUND SUBSTANCE Connective Tissue FIBER MATRIX Fibroblast CELLS Elastic Fiber
  • 12. Teased Dense Regular Connective Tissue • Forms tendons and ligaments and aponeuroses. • Matrix looks white and shiny. • Mostly collagen fibers with fibroblasts between rows. • Strong attachment!
  • 13. Teased Dense Regular CT (magnified) Fibroblasts Fibers are mostly Collagen.
  • 14. Reticular Connective Tissue Reticular Cells • Network of interlacing reticular fibers & Blue staining reticular cells Reticular fibers • Found in stroma (framework) of liver, spleen, lymph nodes, red bone marrow • Acts as a filter in spleen and lymph nodes.
  • 15. Reticular Connective Tissue • Remember that connective tissue is vascular. (Avascular epithelial tissue depends on diffusion of nutrients and waste through blood Blood vessel: Single layer of vessels in CT. Epithelial cells forms vessel tube.
  • 16. Reticular 1 CT 3 • 1- Reticular 2 cells • 2- Blood vessel • 3- Reticular fibers
  • 17. Hyaline Cartilage • Often called gristle • Bluish white smokey chondrocytes looking ground substance • Numerous chondrocytes • Location: ends of long Ground S bones, ribs, nose, trachea, larynx, bronchi • For flexibility and support • Most abundant type of cartilage in body
  • 18. Elastic Cartilage • Chondrocytes are located within threadlike network of elastic fibers • Location: Epiglottis of larynx, external ear, Eustachian tubes • For support and shape
  • 19. Elastic vs Hyaline Cartilage Lacuna (hollow space holding chondrocyte) chondrocyte Smoother Appearance Of ground Elastic fibers throughout substance Ground substance
  • 20. Adipose Tissue • Adipocytes, “signet ring” cells with peripheral nuclei. • For storage of triglycerides, to reduce heat loss, and serve as an energy nucleus reserve. • Location: under skin, around heart & kidneys, yellow “signet ring” marrow, behind adipocyte eyeball
  • 21. Osseous Tissue (Bone) • Compact bone consists of osteons (haversion systems) that contain: – Lamellae – Lacuna – Osteocytes – Canaliculi – Central (haversion)canals • Spongy bone consists of thin plates called trabeculae filled with red marrow
  • 22. Lamellae Central(Haversion) Haversion Canal System Lacunae
  • 23. Lacunae (where osteocytes reside) Canaliculi: small Tunnels where osteocyte processes run for diffusion of nutrients and wastes
  • 24. Skeletal Muscle Tissue • Striated – Proteins • Actin Striations • Myosin • Multinucleated • Voluntary • Attach to bones • For movement, communication, posture, heat production
  • 25. Skeletal muscle fiber with multiple nuclei
  • 27. Smooth (Visceral) Muscle Tissue • No striations • Spindle-shaped • Single centrally located nucleus • Nucleus is long and cigar shaped • Involuntary • Viscera • Walls of hollow structures – Blood vessels – Airways to lungs – Stomach – Intestines – Gallbladder – Urinary bladder
  • 28. Cigar shaped nuclei of smooth muscle tissue
  • 30. Cardiac Muscle Tissue • Striated, mono- nucleated, branching • Self excitatory • Intercalated discs Intercalated disc attach fibers together and contain gap junctions (help with nucleus conduction of electrical signals)
  • 31. Name the tissues and structures you see.