Lab summariesassignment 2 done
- 1. University of Puerto Rico - Cayey RISE Program Template for Laboratory Summaries of Assignment 1Biol. 4997-BiomedicalTechniques Due dateApril5, 2013 Reg.# 3 Name José A. Cruz Arzón Date: April 5, 2013 Summary4. Nanotechnology and Electron MicroscopyFebruary 22, 2013 Nanotechnology is technological use of particles smaller than 10nm. The purpose of this lab is to present a basic concept of nanoparticles, because of its great surface area, its ability of catalysis and chemical reactivity. Nanotechnology is used in biomedicine and can be use for metallic implants for reconstruction of bones using osteoinduction, which stimulates stem cells to become osteoblasts. Using nanotechnology in hydroxyapatite fibers, a bone component, we can help cells to regenerate accepting the implant and avoiding it to corrode. Furthermore, the fibers had to go by several steps; the first one is sputtering which deposits the fibers by shooting electrons toward the fibers using a machine called magnetron sputter system. Then, the fibers go to the metallization process using a vacuum deposition chamber creating an empty space for the fibers to metalize. After that, the fibers are ready to be studied under a TEM microscope. In conclusion, nanotechnology can one day save our lives, thanks to its impact on biomedicine by studying hydroxyapatite fibers. Word Count___167____ Summary5. Column Chromatography and SDS-PageMarch 1, 2013 As main purpose of this lab we learned how to isolate plasminogen activator using magnetic affinity nanoparticles. Proteins in pure isolated form are the ones good for studies. The main reason why magnetically isolating is better than traditional ways is because of their great surface area that lets more ligands to attach extracting more proteins. Another reason is because the ligand contains a high specificity for a protein; this permits us isolate just the protein that we want. To isolate the protein we needed to suspend and equilibrate so the ligand would attach to the MNPs, then bind the protein using HeLa broth from mammalian cell. After that, we did three washes to remove proteins that we did not want and three eluations to eluate the proteins from the MNPs. Then we did a chromatography to measure the absorbance at each volume and an electrophoresis to see how well the proteins were isolated. Future studies can be done to detect miss-expressed genes that let to wrong synthesized by isolating magnetically. Word Count___170____ Summary6. Protein – Protein Interactions: an In silicoapproachMarch 15, 2013 In silico approach allows to study protein interactions in details. The objective is to know about designing, developing and discovering drugs using computer programs. The workshop started with an introduction about how drugs have to pass the FDA before manufacturing. Between preclinical researches, development, clinical trials, and the approval of the drug, there can be from 10 to 20 years before
- 2. manufacturing. After discovered, with in silico we design the drug. Our group was in charge of identifying the target of the protein for drug development. Basically, after downloading the protein we first separate the ligand and the receptor from the protein and studied interactions. After we analyzed the grid, we looked for possible sizes for ligands to fit. Then we saved images of the grid with the ligand, and the protein with ligand and grid. After that, we did the mapping of benzene molecule and analyze it by binding energies and cluster. These techniques can be applied when looking for inhibitors to fit target of proteins of infectious diseases. Word Count___170____