Le cell
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The LE cell demonstration document describes the LE cell, which is a neutrophil that has phagocytosed nuclear material coated with antinuclear antibodies, a characteristic of lupus erythematosus. It discusses several methods for demonstrating LE cells in blood samples, including using clotted blood, defibrinated blood, or the rotary method. The rotary method involves adding glass beads to heparinized blood and rotating at 50rpm for 30 minutes at 37 degrees Celsius before preparing buffy coat smears to identify LE cells.
Le cell
- 2. The lupus erythermatosus (LE) cell: Is a mature neutrophilic polymorphonuclear leukocyte, which has phagocytized a spherical, homogeneous-appearing inclusion, itself derived from nuclear material of degenerating leukocytes and coated with antinuclear antibody; a characterisitic of lupus erythematosus
- 4. LE cell seen in….. • Systemic lupus erythematosis • Rheumatoid arthritis • Discoid lupus erythematosis
- 5. Methods of demonstration of LE cell • Clotted blood • Defibrinated blood • Citrated or heparinized blood • Rotary method
- 6. Clotted blood • Simplest method • 10 ml of clotted blood is placed in water bath at 32o C for 1 to 2hrs. • Mash the clot with fine wire • The cells expressed from the clot are placed in a Wintrobe haematocrit tube and centrifuged at high speed (2,000 r.p.m.) for 5 minutes. • The buffy coat is then ,carefully removed with a fine glass pipette and smeared on glass slides. • Any of the stains for routine blood films are satisfactory.
- 7. Defibrinated blood • The blood is collected in a flexible container (conical flask) of appropriate size into which is placed a sufficient quantity of glass beads. • Blood is added to it. • It is rotated in 8 shape for 15min. • Incubated for 2hrs at 370 • Smears are made after centrifugation same as clotted blood • Stain with wright stain.
- 8. Rotary method • 5 glass beads 3mm in diameter are added to a heparinised sample of blood. • Tube is then roated at 50rpm for 30 min at 37o • Buffy coat smears are prepared.
- 9. • LE cell are seen at the edge of a smear. • 500 polymorhs are counted before a negative result is given. • Rosette – group of polymorhs will collect around altered nuclear material.