Leaf analysis
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This document outlines the methodology for analyzing elements in plant leaves through both dry and wet analysis. Dry analysis uses an elemental analyzer to measure nitrogen, while wet analysis involves ashing leaves in a furnace to convert organic material to inorganic ash that can then be analyzed using an ICP instrument. The wet analysis methodology further describes preparing an ash solution, then a process for analyzing silica levels using a spectrophotometer.
Leaf analysis
- 1. MOHAMAD RAAFI B AHMAD TAIB 152036 (Practical Report Presentation)
- 3. PRINCIPAL Dry (Bomb) analysis Instrument use Elemental Analyzer (EA) For nitrogen Wet analysis (solubility) Organic material need to be converted to inorganic material Ashing (greyish-white) Instrument use furnace and ICP The elements contain in the leaves can be diluted using dilute acid.
- 5. ASHING AND PREPARATION OF ASH SOLUTION Leaf need to be dry and grind to get homogenous. Weigh about 1 to 2 g of the sample into a silica dish. Place the dish in the furnace (500⁰C for 5 hours) until greyish-white
- 6. Add 2 ml Hydrochloric acid and evaporate to dryness on a hot plate. Add 10 ml of nitric acid and digest on a water-bath for 1 hour.
- 7. Filter into a 100 ml volumetric flask using filter paper. Make up to volume with water and mix well.
- 9. PRINCIPAL Wet analysis (solubility) Inorganic material. Use spectrometer uv to get the wavelength of silica (yellow colour).
- 10. Weight sample 0.1g ,put in 250 ml Erlenmeyer flask Add 5g Sodium carbonate Add 100ml distilled water Boil on Hot plate for 15-30 min ANALYSIS OF SILICA
- 11. Pour 5 ml sulfuric acid Boil in water bath (6 hours), leave 2 days Filter samples & dilute 10 times Pipette 10 ml sample & add molybdate, acid reagent and citric acid (shake) Obtain result from spectrophotometer (410 nm yellow wavelength)