Lecture 21 Vibrio cholerae
Download as PPT, PDF5 likes2,224 views
This document summarizes key information about the Vibrionaceae family of bacteria, focusing on the important genera of Vibrio, Aeromonas, and Plesiomonas. It provides details on Vibrio cholerae, the cause of cholera, including its morphology, identification, virulence factors, pathogenesis, diagnosis, treatment and prevention. Information is also given on Vibrio parahaemolyticus and Aeromonas species, noting their associations with seafood, gastroenteritis, and other infections.
Lecture 21 Vibrio cholerae
- 1. بسم ا الرحمن الرحيم FAMILY: VIBRIONACEAE Prof. Khalifa Sifaw Ghenghesh
- 2. 3 Important Genera: • Vibrio • Aeromonas, • Plesiomonas • Oxidase +ve Habitat:
- 3. بسم ا الرحمن الرحيم Vibrio cholerae I. V. cholera O1: • Agglutinate in Antisera Directed Against O1 Antigen. • Produce Enterotoxin. 2. Non-Toxigenic V. cholera O1: 3. Non O1 V. cholera (NCV, NAV): II. V. cholerae O139
- 5. Vibrio cholerae Leifson flagella stain
- 6. Vibrio cholerae curved rods with polar flagellum (EM)
- 7. V. cholerae O1 --------------------------------------------------Biotye Haemolysis (SRBC) VP El-tor + Classic -- + -- Resistance to PB + ----------------------------------------------------
- 8. VIRULENCE FACTORS: • Enterotoxin: CT • Adherence: Ability to Attach to the Microvilli at the Brush Borders of the Epithelial Cells. EPIDEMIOLOGY: • Causes Widespread Disease. • Human Carriers: • Modern International Travel:
- 9. PATHOGENESIS & CLINICAL INFECTION: • • • • • Infectious Dose: Incubation period: Abrupt Onset of Diarrhoea & Vomiting Stool > Watery (Rice Water), Odorless Fatality Rate:
- 10. LAB. DIAGNOSIS: • Isolation & Identification of the Organism. • Media: TCBS • Transport Media: TREATMENT: • Prompt Replacement of Fluid & Elecrolytes • Tetracycline and others PREVENTION & CONTROL: • Adequate Sewage Treatment and Water Purification Systems. • Vaccination: ??
- 11. Typical Vibrio cholera contaminated water supply
- 13. Vibrio parahaemolyticus • Halophilic vibrio • Associated with seafood • Gastroenteritis: – Outbreaks of Food Poisoning • Extraintestinal infections > wounds • Virulence Factors: – Haemolysin > – heat-stable cytotoxin – Heat-labile enterotoxin – Adherence to human intestinal cells
- 14. Aeromonas species A. hydrophila, A. sobria & A. caviae • Enterotoxins, Cytotoxins, Haemolysins, Etc… • Gastroenteritis, Septicaemia, and Skin & Wound Infections TREATMENT • TMP-SMX, Tetracycline, Gentamicin • Resistant to β-Lactam Antibiotics.