Lecture 4.pptx
- 1. CHROMOSOMES
- 2. Introduction • The word chromosome has been derived from two Greek words "Chroma" meaning colour and "Soma" meaning body. • They are the unique cell organelles made up of chromatin material which is the most important and permanent constituent of the cell nucleus. • They are capable of self-reproduction. • They control cell's structure and metabolism. • Play an important role in the differentiation, heredity, mutation and evolution.
- 3. History • Hofmeister in 1848, discovered nuclear filaments in the nuclei of pollen mother cells of Tradescantia. • First accurate count of chromosomes was made by Flemming in 1882, in the nucleus of a cell. • In 1884, W. Flemming, Evan Beneden and E. Strasburger demonstrated that the chromosomes double in number by longitudinal division during mitosis. • Beneden in 1887 found that the number of chromosomes for each species was constant. • The term "Chromosomes" was coined in 1888 by Waldeyer for the nuclear filaments. • W.S. Sutton and T. Boveri suggested the role of chromosomes in heredity in 1902, which was confirmed by Morgan in 1933.
- 4. Structure of Chromosomes It has three types: 1. Viral Chromosomes. 2. Prokaryotic Chromosome. 3. Eukaryotic Chromosome.
- 5. Viral Chromosome • In viruses there is a single chromosome bearing a single nucleic acid molecule (DNA or RNA) surrounded by a protein coat called Capsid. • It may be linear or circular. • The viruses having DNA as genetic material are called DNA viruses and those having RNA as genetic material are known as RNA viruses. • A limited amount of genetic information is present in the viral chromosome which codes for little more than the production of more virus particles of the same kind in the host cell. • In RNA viruses, often the RNA directs the synthesis of DNA complementary to itself by reverse transcription in the host. • The RNA is then transcribed by the DNA for the formation of new virus particles. Such ribo-virus is called retrovirus. The AIDS causing virus is a retrovirus.
- 6. Prokaryotic Chromosomes • Prokaryotic chromosome (e.g., bacteria) has a single and circular two- stranded DNA molecule which is not enveloped by any membrane. • It lacks proteins and is in direct contact with the cytoplasm. • In addition to the main chromosome some extra-chromosomal DNA molecules may also be present in most of the bacterial cells they are also double stranded and circular, but are much smaller in size. They are known as plasmids. • The plasmid may occur independently in the cytoplasm or may also be found in association of main chromosomal DNA and be called as episome.
- 7. Eukaryotic Chromosomes • The eukaryotic chromosomes are present in nucleus and in certain other organelles, like mitochondria and plastids. • These chromosomes are called nuclear and extra nuclear chromosomes respectively. • Nuclear chromosomes are double stranded long DNA molecules of linear form. Proteins are associated with them. They are surrounded by nuclear envelope. • Extra nuclear chromosomes are present in mitochondria and plastids. • They are double stranded short DNA molecules of circular form. • They lack protein association.
- 8. Morphology of Chromosomes • During the interphase stage, the eukaryotic chromosomes are extended into long and thin chromatin fibers where they lie criss- cross to form the chromatin reticulum. • They replicate in the S-phase and become double. At this stage they consist of two chromatids that are held together at one point called centromere. • At the time of cell division, the chromosomes condense and tightly coil up and become distinct at metaphase stage. • The eukaryotic chromosomes vary in number, size, shape and position but they have remarkably uniform structure.
- 9. 1. Number: - Eukaryotic chromosomes vary in number from two to a few hundred in different species. -In a species all the individuals have same number of chromosomes in all of their cells, except the gametes. - Since the chromosome number is constant for a species, it is helpful in determining the phylogeny and taxonomic position of the species.
- 10. 2. Size: - In a species all the chromosomes are not of the same size. - Their size also varies from species to species. - The particular chromosome of a species however has more or less a constant size. - The organisms having fewer chromosomes have large sized chromosomes than those having many. - Generally, plant chromosomes are larger than animal chromosomes and among plants the monocots have larger chromosomes than the dicots.
- 11. 3. Shape: - The chromosomes at metaphase stage look like slender rods that may be straight or curved to form an arc or a letter S. - In anaphase stage they may assume J or V shapes, depending upon the position of the centromere.
- 12. 4. Position: - In a nucleus each chromosome is independent of all the other chromosomes in its location. - Thus, they may occupy any region of the nucleus.
- 13. 5. Structure: - At metaphase stage, since the chromosome is a highly condensed nucleoprotein filament, it contains two greatly coiled sister chromatids. - These chromatids that lie side by side along their length, are held together at a point called centromere, an area of the narrow region also called primary constriction of the metaphase chromosome. - At the centromere each chromatid has a darkly staining, disc like, fibrous structure, called kinetochore, to which spindle microtubules attach during cell division. - Kinetochores are the sites where force is exerted to pull the chromatids towards the poles.
- 14. - One or more chromosomes may have additional narrow regions called the secondary constrictions. - The part of the chromosome separated by secondary constrictions is termed as satellite. - A chromosome with a satellite is called sat chromosome. - The size and the shape of the satellite remain constant for a species. - Secondary constrictions are associated with the nucleoli, and are known as the nucleolar organizers. - The chromosomes which have nucleolar organizing regions are known as the nucleolar chromosomes.
- 15. 6. Ends: - The ends of chromosomes are called telomeres. - The function of telomere varies from the rest of the chromosome. - On exposure to X-rays a chromosome may break and its pieces may re-join, but no segment connects to the telomere, showing that the telomere has a polarity, and it, somehow "seals" the end.
- 16. 7. Chemical composition: The chromatin in the eukaryotic chromosome consists chemically of about: - 35% DNA. - 60% proteins. - 5% RNA. - Some metal ions and certain enzymes.
- 19. Types of Chromosomes • On the basis of position and number of centromeres, the chromosomes are classified as: - Metacentric. - Submetacentric. - Acrocentric. - Telocentric. - Acentric. - Monocentric. - Dicentric.
- 20. Metacentric • In metacentric chromosomes the centromere is at the middle of the chromosome, and the arms are equal. • In anaphase the chromosome appears V-shaped. • For example: Human chromosome no. 3.
- 21. Submetacentric • The centromere is near to the centre of the chromosome, and the arms are slightly unequal. • In anaphase the chromosome appears J or L shaped. • For example: Human chromosome no. 1.
- 22. Acrocentric • In this type the centromere is near to one end of the chromosome, and the arms are very unequal. • For example: Human chromosome No. 4 & 5.
- 23. Telocentric • The centromere is at one end in such chromosomes, and the arms are on one side only. • The chromosome remains rod shaped in anaphase also.
- 24. • Depending upon the number of centromeres there are three types of chromosomes: (i) Acentric: The chromosome is without a centromere, which is formed by breakage of the chromosome. It does not attach to spindle microtubules so it is lost in the cell division. (ii) Monocentric: It is the chromosome with a single centromere and it is the most common type. (iii) Dicentric: It is the chromosome with two centromeres and is formed by the fusion of two chromosome segments each having a centromere. It is unstable and may break when the two centromeres are pulled to opposite poles in mitosis.
- 25. Giant Chromosomes • Giant chromosomes are special, enormously enlarged chromosomes about 100 times thicker than the ordinary mitotic chromosomes. • These are seen in certain tissues of varied groups of animals and plants. • They are easily visible under light microscope. • The giant chromosomes are of two types: 1. Polytene. 2. Lampbrush.
- 27. Visual Aid • https://www.youtube.com/watch?v=2jGMEcr2g_4 • https://www.youtube.com/watch?v=mh7i3nhzXzk&t=33s
- 28. CELL CYCLE
- 29. Cell Cycle Stages, Mitosis & Cytokinesis
- 30. Interphase • The time between the end of telophase and the beginning of the next M phase is called the interphase. • It is a long stage that lasts for 10 to 30 hours. • During this phase the cell grows by synthesizing biological molecules such as lipids, proteins, carbohydrates, nucleic acids. • Interphase is further divided into three sub phases or periods: - First gap or G1 phase. - Synthetic or S phase. - Second gap or G2 phase.
- 31. G1 Phase • The gap between previous mitosis and beginning of DNA synthesis is represented by G1 phase. • In this stage initial growth of a newly formed cell takes place. • Various biological molecules (carbohydrates, proteins, lipids, including some non-histones, RNAs) are synthesized in this phase. • Normal metabolism is carried out for the preparation for DNA replication that is to take place next to it. • DNA synthesis does not occur in this phase.
- 33. S Phase • During this phase duplication of each chromosome take place by replication of new DNA molecule on the template of the existing DNA. • Synthesis of histone proteins and their mRNA, some non-histone proteins and formation of new nucleosome also occur in S-phase only. • In most of the eukaryotes the S-phase lasts for 6 to 8 hours.
- 34. G2 Phase • G2 phase is the gap between DNA synthesis and nuclear division. • RNA transcription and protein synthesis continues during this phase. • Further growth of the cell and preparation for its division also takes place in this stage. • During this stage the cytoplasmic organelles such as centrioles, mitochondria and Golgi apparatus are doubled, proteins for spindle are synthesized and active metabolism stores energy for mitosis. • The G2 phase in most cells lasts for 2 to 5 hours.
- 35. Mitotic Phase • Interphase is followed by mitotic phase. • During mitotic phase the already duplicated chromosomes are equally distributed to the daughter cells which contain exactly the same hereditary information as the parent cell. • Though, the other cell components (organelles and molecules) are also divided approximately equally between the daughter cells, but not as precisely as the DNA. • After the mitosis is over, the daughter cells enter the G1 phase of the next cell cycle. • During mitosis many structural and physiological changes take place in the cell, as the chromatin of the nucleus is packed into visible chromosomes, which are set free by breakdown of nuclear envelope. • An extensive reorganization of the membranous components and cytoskeletal elements takes place.
- 36. • Endoplasmic reticulum and Golgi apparatus break down into small vesicles and stops the protein movement. • Microtubules dissociate into tubulin dimers and are assembled into the spindle which occupies most of the cell and helps in the distribution of chromosomes into the daughter cells. • Actin filaments get reorganized and form a contractile ring for the cytoplasmic division.
- 38. Mitosis • A German biologist Eduard Strasburger described mitosis for the first time in 1875. • Same was described later in 1879 by Walther Flemming who also termed it "mitosis" in 1882. • It is the most common method of cell division in eukaryotes that takes place in somatic cells of the body and hence it is also known as somatic division. • In plants it takes place in the cells of meristematic tissues. • The duration of mitosis on an average is from 30 minutes to 3 hours.
- 39. • Mitosis is defined as the division of a parent cell into two identical daughter cells. • With same amount of DNA in the nucleus and the same number and kind of chromosomes. • There are two main events involved in mitosis: - Karyokinesis: Division of the nucleus. - Cytokinesis: Division of cytoplasm.
- 41. Karyokinesis • In eukaryotes, karyokinesis is a complex process due to the presence of many chromosomes. • It is a continuous process which may be divided into four stages: - Prophase. - Metaphase. - Anaphase. - Telophase.
- 42. Prophase • In an interphase cell the chromosomes are greatly extended and spread throughout the space in the nuclear compartment. • Approximately 4 meters of DNA is organized into 46 duplicated chromosomes is present in the nucleus of a human G2 cell. • The prophase is long and complex that lasts for about 50 minutes. • It is divided into 3 sub stages: - Early prophase. - Middle prophase. - Late prophase.
- 44. • In early prophase, the cell starts to break down some structures and build others up, setting the stage for division of the chromosomes. • The chromosomes start to condense (making them easier to pull apart later on). • The mitotic spindle begins to form. • The spindle is a structure made of microtubules, strong fibers that are part of the cell’s “skeleton.” • Its job is to organize the chromosomes and move them around during mitosis. The spindle grows between the centrosomes as they move apart. • The nucleolus-a part of the nucleus where ribosomes are made, disappears. This is a sign that the nucleus is getting ready to break down.
- 46. • In late prophase (sometimes also called prometaphase), the mitotic spindle begins to capture and organize the chromosomes. • The chromosomes become even more condensed, so they are very compact. • The nuclear envelope breaks down, releasing the chromosomes. • The mitotic spindle grows more, and some of the microtubules start to “capture” chromosomes.
- 47. Metaphase • In metaphase, the spindle has captured all the chromosomes and lined them up at the middle of the cell, ready to divide. • All the chromosomes align at the metaphase plate. • At this stage, the two kinetochores of each chromosome should be attached to microtubules from opposite spindle poles. • Before proceeding to anaphase, the cell will check to make sure that all the chromosomes are at the metaphase plate with their kinetochores correctly attached to microtubules. This is called the spindle checkpoint. • It helps ensure that the sister chromatids will split evenly between the two daughter cells when they separate in the next step. • If a chromosome is not properly aligned or attached, the cell will halt division until the problem is fixed.
- 49. Anaphase • In anaphase, the sister chromatids separate from each other and are pulled towards opposite ends of the cell. • The protein “glue” that holds the sister chromatids together is broken down, allowing them to separate. Each is now its own chromosome. The chromosomes of each pair are pulled towards opposite ends of the cell. • Microtubules not attached to chromosomes elongate and push apart, separating the poles and making the cell longer. • All of these processes are driven by motor proteins, molecular machines that can “walk” along microtubule tracks and carry a cargo. • In mitosis, motor proteins carry chromosomes or other microtubules as they walk.
- 51. Telophase • In telophase, the cell is nearly done dividing, and it starts to re- establish its normal structures as cytokinesis (division of the cell contents) takes place. • The mitotic spindle is broken down into its building blocks. • Two new nuclei form, one for each set of chromosomes. Nuclear membranes and nucleoli reappear. • The chromosomes begin to decondense and return to their “stringy” form.
- 53. Cytokinesis • Cytokinesis, the division of the cytoplasm to form two new cells, overlaps with the final stages of mitosis. • It may start in either anaphase or telophase, depending on the cell, and finishes shortly after telophase. • In animal cells, cytokinesis is contractile, pinching the cell in two. • Plant cells can’t be divided like this because they have a cell wall and are too stiff. • Instead, a structure called the cell plate forms down the middle of the cell, splitting it into two daughter cells separated by a new wall.
- 55. Significance of Mitosis • Maintenance of Size: Mitosis helps maintaining the size of the cell. A cell, when full grown, divides by mitosis instead of growing further. • Growth: A fertilized egg develops into an embryo and finally into an adult by repeated mitotic cell division. • Maintenance of Chromosome Number: Mitosis keeps the number of chromosomes equal in all the cells of an individual. Thus mitosis provides a complete set of genetic information to each cell, since DNA is duplicated in S phase prior to mitosis. • Repair: Mitosis provides new cells to replace the old worn out and dying cells. • Healing and Regeneration: Mitosis produces new cells for the healing of wounds and regeneration. • Reproduction: Mitosis brings about multiplication in the acellular organisms. In multicellular organisms also, it plays an important role in reproduction, asexual as well as sexual. • Evidence of Basic Relationship of Organisms: Mitosis, being essentially similar in many kinds of organisms, supports the basic relationship of all living things.