Linked In March24v5
- 1. Engineer “Arg-Gly-Asp” (RGD) epitopes for multi-valent display
- 2. RGD: stem-cell adhesion epitope also target cancer cells that up-regulate integrin TYMV with RGD substitution at residue 44
- 3. pH-dependent enhanced stability Mutate internal scaffold N-terminus of TYMV CP Wild-type 70°C N-term mutant 70°C
- 4. Robust TYMV virus- like particles in E. coli In vitro reassembly from a denatured state
- 5. After many months crystal structure data for TYMV and mutants!
- 6. TYMV can form a molecular “container” after release of RNA, nano-inspired applications with a protein shell with a small “hole” before After freeze-thaw
- 7. A CP cleavage product formed during RNA ejection hindered in one of our “super-stable” virion mutants &! SDS-PAGE: Coomassie stain ( ! Wt Mass Spec after freeze- 26 17 % RNA release ! C thaw: intact particle ! "# $! !!ATC Virion ATC Virion / 01! / 23! Wt Ky &! SDS-PAGE: Coomassie stain (! ) *&) !!!*+, !!!&*, - , . , +! Western blot ' ! 26 1 min. / 01! %! exposure Intensity 17 ! "#$! !!ATC Virion ATC%Virion ! / 01! / 23! Wt Ky min. 10 exposure ) *&) !!!*+, !!!&*, - , . , +! / 23! ! Western blot 'pH 11.5 pH 4 pH11.5 pH 1 min. 4 %! / 01! exposure Intensity Ky Wt % min. ! +132 +132 10 # !Coomassie SDS-PAGE exposure / 23! +132 pH 11.5 pH 4 pH11.5 pH 4 %! 13 % ! 12 Ky 14Wt 15 16 Mass (Da) +132 +132 Fourier Transform-Ion Cyclotron Resonance Wt CsCl gradient fractions Coomassie SDS-PAGE #! +132 (FTICR) Mass spectrometry
- 8. TYMV dual-subgenomic RNA for expression of GFP and GUS. Future downstream use for vaccine development
- 9. Biophysical studies of TYMV Characterization empty, virion and intermediate particles Northerns, Westerns, gradient centrifugation, etc.
- 10. Previous Work (M.S degree at OHSU): Signaling of a Cytomegalovirus chemokine receptor US28: plenty of cloning, tissue culture, immuno-precipitation/fluorescence and construction of CMV knockout mutants US28 chemokine
- 11. Recent TYMV article & (cover image) Jan 2011, more publications to come