Methods of microbiolgy
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This document discusses isolation and maintenance of microbial cultures. It describes the history of isolating bacteria in pure culture between 1876-1884 by Robert Koch. The three main methods of isolation are streak plating, spread plating, and pour plating. Streak plating dilutes microbes to isolate individual colonies. Spread plating spreads an inoculum uniformly over solid medium. Pour plating adds culture to molten agar before pouring in a petri dish. Microbial cultures can be maintained through lyophilization, agar slants, refrigeration, or cryopreservation. Preservation has applications in academia, research, fermentation industry, and biotechnology.
Methods of microbiolgy
- 1. ISOLATION AND MAINTENANCE OF MICROBIAL CULTURE PRESENTED BY Atish Kumar
- 2. CONTENTS Histroy Isolation Methods of isolation Maintenance of microbial culture Applications
- 3. Important terms keep in our mind I. What is that II. Why we do III. How we do IV. Type of medium V. Type of organisms VI. Site VII. Aim
- 4. HISTROY In year between 1876 AND 1884 ROBERT KOCH was the first scientist to focus on isolation of bacteria in pure culture This will give rise to ‘Germ theory’. According to this a certain micro organisms is responsible for certain diseases .
- 5. ISOLATION :- IN MICROBIOLOGY ISOLATION REFERS TO GETTING A PURE CULTURE. There are three basically methods of Isolation :- (i)streak plate (ii)spread plate (iii)pour plate
- 6. STREAK PLATE THEORY:- This is essentially a method to dilute the number of organisms, decreasing the density - individual colonies to be isolated from other colonies. Each colony is "pure," since theoretically, the colony began with an individual cell
- 7. 1.) Flame the loop, cool by touching an uninoculated portion of the surFace. 2.) Now rotate the plate. Open lid and streak again, remember: you are picking up growth from quadrant one, and using this as your inoculum for quadrant two. 3.) Flame loop; rotate plate, and repeat procedure for quadrants three and four.
- 9. This is an example of a good streak for isolation using the "four corners" method.
- 10. (II) SPREAD PLATE THEORY :- A serailly diluted inoculum is transferred over the surface of the solid medium and then spreaded uniformly with a sterile bent glass spreader. Colonies can be easily isolated from this method.
- 12. POUR PLATE THEORY:- In pour plate , a small amount of culture is added by pipette to the cenre of petri dish .cooled ,but molten agar medium in a test tube or bottle is then poured in petridish . The dish is then rotated gently ,to ensure the culture is mixed properly
- 13. MAINTENANCE OF MICROBAIL CULTURE By lyophilization By agar slants By parafin method By refrigeration By cryopeservation method
- 14. By lyophilization By parafin method
- 15. By refrigeration By cryopreservation
- 16. APPLICATIONS OF PRESERVATION Academic Use Research purpose Fermentation industry Biotechnological field