Microscope iii
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The document provides an overview of fluorescence microscopy techniques, highlighting its various applications such as determining protein localization, studying dynamics, and examining cellular structures. It discusses different types of light sources and the basic design of fluorescence microscopes. The document also mentions various filter and objective lens configurations used in microscopy.
Microscope iii
- 1. Microscope-III Md. Saiful Islam B.Pharm, M.Pharm (PCP) North South University Fb Group: Pharmacy Universe
- 2. A reminder… • Brightfield - absorption • Darkfield - scattering • Phase Contrast - phase interference • Fluorescence Contrast - fluorescence
- 3. What can you do with a fluorescence microscope? •Determine the localisation of specific (multiple) proteins •Determine the shape of organs, cells, intracellular structures •Examine the dynamics of proteins •Study protein interactions or protein conformation •Examine the ion concentration. can observe in live cells
- 4. upright microscope light path
- 5. upright microscope light path Lamp Filter cube Objective lens sample Camera For bright field microscopy
- 6. Basic design of fluorescence Objective acts as condenser; excitation light reflected away from eyes
- 7. Light source for fluorescence microscopy Arc lamps Xenon Mercury UV IR Laser types Argon 351 364 457 477 488 514 Blue diode 405 440 Helium-Cadmium 354 442 Krypton-Argon 488 569 647 Green Helium-Neon 543 Yellow Helium-Neon 594 Orange Helium-Neon 612 Red Helium-Neon 633 Red diode 635 650 Ti:Sapphire 720-980