Mol bio
- 2. • Melting temperature of DNA in different organism ranges from • 30-60% • 20-30% • 33-75% • 66-95%
- 3. • 33-75%
- 4. • AMBER refers to the codon • UAA • UAG • UGA • All of these
- 5. • UAG
- 6. • . Which amino acids has single codon? 1. Tyrosine & methionine 2. Cysteine & methionine 3. Arginine & methionine 4. Tryptophan & methionine
- 7. • Tryptophan & methionine
- 8. What is m-RNA splicing a.Addition of intron b.Addition of exon c.Removal of intron d.Removal of exon
- 9. • Removal of intron
- 10. • After maturation hn RNA gets converted into • r-RNA • t-RNA • m-RNA • Si RNA
- 11. • m-RNA
- 12. • r RNA is product of 1. RNA polymerase I 2. RNA polymerase II 3. RNA polymerase III 4. RNA polymerase IV
- 13. • RNA polymerase I
- 14. • RNA polymerase I is present in • 1. Cytoplasm 2. Mitochondria 3. Nucleoplasm 4. Nucleolus
- 15. • Nucleolus
- 16. • RNA polymerase II is present in a.Cytoplasm b.Mitochondria c.Nucleoplasm d.Nucleolus
- 17. • Nucleoplasm
- 18. • The role of RNA polymerase subunit β’ is 1. Promoter binding 2. Nucleotide binding 3. DNA template binding 4. Initiation
- 19. • DNA template binding
- 20. • The role of RNA polymerase subunit β is a.Promoter binding b.Nucleotide binding c.DNA template binding • Initiation
- 22. • In phage T2 virus the DNA is estimated to be about 200,000 deoxyribo nucleotide base pairs then what is the length in micrometer of its DNA complement. a.2.0 µm b.3.4 µm c.68 µm d.34 µm
- 23. • 68 µm
- 24. • The role of RNA in DNA replication is a.Unwinding the helix of DNA b.Activation of DNA polymerase c.Prevention of two helix to bind each other d.Act as primer
- 25. a.Act as primer
- 26. • 33. Ratio of the bases present in different samples of nucleic acid yielded the following results 1. (A+C)/ (T+G) = 1 2. (A+C)/ (U+G) = 0.8 3. (A+G) / (T+C) =1.5 • Among these conclude which one is single standed RNA and which one is single standed DNA a. All three are single standed DNA b. 2 is SS RNA & 3 is SS DNA c. 1 is SS DNA & 2 is SS RNA d. None of the above
- 27. • 2 is SS RNA & 3 is SS DNA
- 28. • . In lac operon transports of lactose into the cell is due to a. β-galactosidase b. Permease c. Transacetylase d. Both a and b
- 29. • Permease
- 30. • . The form of DNA with left hand rotation is 1. A form 2. B form 3. D form 4. Z form
- 31. • Z form
- 32. • . The form of DNA present in human is a.A form b.B form c.D form d.Z form
- 33. • B form
- 34. • Repressors bind to specific sites on the DNA. In prokaryotic cells, such binding sites, called • a. Inducer • b. Promoter • c. Operator • d. repressor
- 35. • Operator
- 36. 1. Gene whose product is required at all time • a. Housekeeping gene • b. Junk gene • c. Regulatory gene • d. Promoter gene
- 38. 1. Each cell in an individual with Down syndrome contains --- chromosomes. a. 45 b. 47 c. 22 d. 24
- 39. • 47
- 40. 1. Chargaff found that for DNA a. The ratio of A to C is close to 1:1 and the ratio of G to T is close to 1:1 b. The ratio of A to T is close to 1:1 and the ratio of G to C is close to 1:1 c. The ratio of A to G is close to 1:1 and the ratio of T to C is close to 1:1 d. A + T = G + C
- 41. • The ratio of A to T is close to 1:1 and the ratio of G to C is close to 1:1
- 42. 1. A segment of DNA that reads the same from forward and backward is called a. Junk DNA b. Palindromic DNA c. Haggles DNA d. Probe
- 44. 1. A polycistronic mRNA can be seen in a. Mitochondria b. Chloroplast c. Eukaryotes d. Prokaryotes
- 45. a. Prokaryotes
- 46. 1. polyA tail is present at a.3’ of mRNA b.5’ of mRNA c.3’ of miRNA d.5 of miRNA
- 47. • 3’ of mRNA
- 48. 1. Self-splicing can occur in a. Some precursors of tRNA b. Some precursors of rRNA c. Some precursors of mRNA d. hn RNA
- 49. • Some precursors of rRNA
- 50. • NADH2 after converting into NAD produces how many ATP a. 2 b. 3 c. 4 d. 5
- 51. • 3
- 52. • FADH2 produces how many ATP a. 2 b. 3 c. 4 d. 5
- 53. • 2
- 54. • Which RNA is most unstable in nature? • a. mRNA • b. tRNA • c. rRNA • d. SiRNA
- 55. • . mRNA
- 56. • The controversy regarding the use of Bt corn is that it 1. Is potentially harmful to monarch butterflies 2. Is a potential allergen to humans 3. Both a and b 4. contaminate groundwater
- 57. • Both a and b
- 58. 1. Si RNAs interfere at a.Transcriptional level b.Translational level c.Post transcriptional level d.Post translational level
- 59. • Post transcriptional level
- 60. 1. Inhibitory action of penicillin is a.Cell wall synthesis b.Protein synthesis c.Ribosome subunit synthesis d.RNA synthesis
- 61. • Cell wall synthesis
- 62. 1. During agrobacterium infection the plant cell begins to synthesize Arginine derivatives called as a.Octapine b.Opines c.Vir protein d.Acetosyringone
- 63. • Opines
- 64. 1. Which of the following Vir gene is acetosyringone receptor a.VirA b.VirB c.VirE d. VirC
- 65. • VirA
- 66. 1. Size of Ti plasmid of Agrobacterium tumefaciens is a.100kb b.150kb c.200kb d.250kb
- 67. a.200kb
- 68. 1. Gene produced for recombinant DNA technology contains a gene from one organism joined to the regulatory sequence of another gene. Such a gene is called a. clone b. junk gene c. chimeric gene d. assembly
- 69. a. chimeric gene