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This document provides information about Murashige and Skoog (MS) plant tissue culture media products offered by PhytoTechnology Laboratories. It describes the company's large selection of MS-based media, manufactured according to cGMP procedures. The media are tested for quality specifications and plant compatibility. Key features include standardized components, large batch sizes, custom packaging and formulations, and a media reservation program. A table lists various MS-based media products with their compositions.
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- 1. PhytoTechnology Laboratories® Helping to Build a Better Tomorrow through Plant Science™ Product Information Sheet Murashige and Skoog Media PhytoTechnology Laboratories® offers the largest selection of Murashige and Skoog-based media available to plant tissue culturists. These media range from the basic salt formulations to media complete with gelling agents, vitamins, carbohydrates and plant growth regulators. All of our media are manufactured according to cGMP’s procedures in our environmentally controlled manufacturing facility in Overland Park, KS. Each medium is then tested for specific physio-chemical specifications. The medium is also biologically tested with two commercially significant plant cell lines. PhytoTechnology Laboratories® is committed to maintaining inventory of its entire plant tissue culture media. Some features of our manufactured media include: • All media components meet USP or ACS quality standards where applicable • PhytoTechnology Laboratories® has the capacity to manufacture lots of certain media in up to 50,000 liters batch sizes. • Using Powder Media simplifies media preparation and reduces technician error when preparing media batches. • PhytoTechnology Laboratories® can custom package your media into sizes that fit your normal batch production of media. This reduces time in weighing and allows you to simply open the bottle and pour out the entire contents. • Using PhytoTechnology Laboratories® Lot Reservation Program for manufactured media allows you to use the same lot of medium for up to one year. This reservation program is offered to you at no additional cost. • Custom liquid and powdered media can be manufactured for you using your confidential formulation in lots ranging from 100 liters up to 25,000 liters. Contact us for more details. • Powdered Media has a shelf life of 2-3 years whereas stock solutions should be made fresh every 3-6 months. • There is no minimum order with PhytoTechnology Laboratories®. Note: Linsmaier & Skoog (LS) Medium contains the same inorganic salts as Murashige & Skoog (MS) Medium. LS Medium differs from MS Medium ONLY in the vitamin content. The composition of vitamins in each basal medium is noted after the MS-based media table on the following pages. PhytoTechnology Laboratories® P.O. Box 12205; Shawnee Mission, KS 66282-2205 Phone: 1-888-749-8682 or 1-913-341-5343; Fax: 1-888-449-8682 or 1-913-341-5442 Web Site: www.phytotechlab.com © 2010 PhytoTechnology Laboratories® Product Information Sheet--MS Media rev 3-2010 Page 1 of 3
- 2. PhytoTechnology Laboratories® Helping to Build a Better Tomorrow through Plant Science™ Product Information Sheet The following table is provided to aid in the selection of MS-based media for use in your research or micropropagation applications. Consult the Product Information Sheet available on the product’s web page for complete composition Prod. Sucrose Agar IAA NAA 6-BA 2iP Kinetin Product Description Vitamins Other No. g/L g/L mg/L mg/L mg/L mg/L mg/L Linsmaier & Skoog L467 30.0 7.0 LS Medium Linsmaier & Skoog L689 LS Basal Salts Modified MS Basal M153 ½ Macro and ½ Micros Salts ½ Ammonium Nitrate, Modified MS Basal M290 ½ Potassium Nitrate, Salts ½ Calcium Chloride MS Modified Basal M401 30.0 MS 0.1 1.0 Medium MS Basal Medium w/ M404 Gamborg’s Basal Salt Gamborg Vitamins Modified MS Basal Contains no Nitrogen, M407 Salt Mixture Potassium or Phosphorous MG Basal Salt Modified Murashige/ M419 Mixture Gamborg’s Basal Salts MS Macronutrient Macronutrients (Salts) M502 Salt Base Only Murashige Modified Contains 255.0 mg of M508 Fern Multiplication LS 0.1 2.0 Sodium Phosphate Medium Murashige Modified Contains 85.0 mg Sodium Gerbera Phosphate; 80.0 mg M509 MS 0.5 10.0 Multiplication Basal Adenine Hemisulfate; 100 Medium mg L-Tyrosine Murashige Modified Contains 85.0 mg Sodium M510 Gerbera Pretransplant MS 10.0 Phosphate; 100 mg L- Basal Medium Tyrosine Murashige Modified Kalanchoe M511 LS 3.0 Multiplication Basal Medium Murashige BC Potato Modified M516 0.04 Basal Medium MS Murashige Modified African Violet/ Contains 170.0 mg Sodium M517 Gloxinia LS 2.0 2.0 Phosphate, 80.0 mg Multiplication Basal Adenine Hemisulfate Medium Murashige Modified African Violet/ LS 1.0 M518 Gloxinia Pretrans- plant Basal Medium MS Basal Medium w/ M519 MS Vitamins PhytoTechnology Laboratories® P.O. Box 12205; Shawnee Mission, KS 66282-2205 Phone: 1-888-749-8682 or 1-913-341-5343; Fax: 1-888-449-8682 or 1-913-341-5442 Web Site: www.phytotechlab.com © 2010 PhytoTechnology Laboratories® Product Information Sheet--MS Media rev 3-2010 Page 2 of 3
- 3. PhytoTechnology Laboratories® Helping to Build a Better Tomorrow through Plant Science™ Product Information Sheet MS-based media continued Prod. Sucrose Agar IAA NAA 6-BA 2iP Kinetin Product Description Vitamins Other No. g/L g/L mg/L mg/L mg/L mg/L mg/L M524 MS Basal Salts Inorganic Salts Only Murashige Modified M527 Multiplication Basal LS 0.3 1.0 Medium Liquid Form; MS Micronutrient M529 Micronutrients (Salts) Stock Solution Only Modified MS Basal M531 Contains No Nitrogen Salt Mixture M533 MS Vitamin Powder MS Powder Form; 1000X MS Modified Basal Contains 80.0 mg M535 LS Medium Adenine Hemisulfate Contains 170.0 mg MS Modified Sodium Phosphate, 80.0 M536 Multiplication Basal mg Adenine Hemisulfate, Medium and 0.4 mg Thiamine Modified MS Basal Does not contain M541 Medium Potassium Phosphate Powder Form; MS MS Modified Modified M547 Vitamins w/ 1,000 mg Vitamin Powder MS Thiamine; 1000X M553 MS Vitamin Solution MS Liquid Form; 1000X MS Micronutrient Micronutrients (Salts) M554 Salt Base Only MS Modified Contains 148.0 mg M555 Multiplication 30 LS 0.1 1.0 Sodium Phosphate; 80.0 Medium mg Adenine Hemisulfate Liquid Form; 1000X; MS Modified Modified M557 MS Vitamins w/ Vitamin Solution MS 1,000 mg Thiamine Contains ½ Ammonium Modified MS Basal M561 Nitrate and ½ Potassium Salt Mixture Nitrate Modified MS Basal Does not contain M571 Salt Mixture Ammonium Nitrate Liquid Form; MS Macronutrient M654 Macronutrients (Salts) Stock Solution Only M701 MS Modified Basal Contains 30 mg Adenine LS 1.0 10.0 (M506) Medium Hemisulfate Contains 148 mg Sodium MS Modified Basal M702 30 LS 0.3 Phosphate and 80 mg Medium Adenine Hemisulfate MS Vitamins contains 2.0 mg Glycine, 0.5 mg Nicotinic Acid, 100 mg myo-Inositol, 0.5 mg Pyridoxine HCl, and 0.1 mg Thiamine HCl. LS Vitamins contains 100 mg myo-Inositol and 0.1 mg Thiamine HCl. Gamborg Vitamins contains 1.0 mg Nicotinic Acid, 100 mg myo-Inositol, 1.0 mg Pyridoxine HCl, and 10.0 mg Thiamine HCl. PhytoTechnology Laboratories® P.O. Box 12205; Shawnee Mission, KS 66282-2205 Phone: 1-888-749-8682 or 1-913-341-5343; Fax: 1-888-449-8682 or 1-913-341-5442 Web Site: www.phytotechlab.com © 2010 PhytoTechnology Laboratories® Product Information Sheet--MS Media rev 3-2010 Page 3 of 3