SEMINAR REPORT ON GENETICALLY
MODIFIED CROP
SUBMITTED TO
I.K.GUJRAL PUNJAB TECHNICAL UNIVERSITY
KAPURTHALA, PUNJAB
BY
(2017-2019)
SAKSHI THAKUR
REG NO. 1732065
DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY
GENETICALLY MODIFIED FOOD
What Are Genetically Modified Foods
•Any food containing or derived from a
genetically engineering organisms (plants,
animals or microorganisms).
Background
• As we entered the 21st century, industrialized countries struggling with side effects
of,
‘GREEN-REVOLUTION’.
• Worse, many of the world’s poor have never benefitted from Green Revolution because it
did not solve underlying problem,
‘POVERTY’
• Now, WHAT CAN HELP THEM?
 Many scientist think a new ‘GENE REVOLUTION’, uses biotechnology to create a new
‘Genetically Modified’ or ‘GM CROPS’.
• But, some people worry that whether,
‘These crops are safe or not’
Source: www.biotechinstitute.org/download/files/YourWorld/yw-101-food
GM FOOD FROM GM CROPS
HOW GENETICALLY MODIFIED ORGANISMS PRODUCED?
• Genetically Modified Organisms (GMOs) are being made by inserting a gene from
an external source such as viruses, bacteria, animals or plants into usually
unrelated species.
Contd…. Types of Crop
FRUITS
VEGETABLES
ORNAMENTALS
PLANTATION CROPS
• Genetically modified crops or
Transgenic crops carrying the novel traits
and released for commercial agriculture
production.
• These include:
Pest resistant cotton, maize, canola (mainly Bt or
Bacillus thuringiensis), herbicide glyphosate resistant
soybean, cotton and viral disease resistant potatoes,
papaya and squash.
GENETICALLY MODIFIED
CROPS
VIRUS
TOLERANCE
DROUGHT
RESISTANCE
GENERATION OF GMOs
FIRST GENERATION SECOND GENERATION THIRD GENERATION
 Enhance crop productivity
 Traits such as herbicide
tolerant, better insect
tolerant, and better
tolerance to environmental
stress.
 They are not significantly
different from traditional
crops in terms of
appearance, taste and
nutrition.
 Examples: Corn, soybean
etc.
 Concerns product quality
characteristics such as
having better taste, more
nutritional content, and
having longer shelf life.
 Example: Flavr savr
tomato, Potato.
 Use of plants to make cheaper, more efficiently produced
and more plentiful industrial products, such as biosensors,
industrial enzymes and epoxies, plastics and cosmetics, and
pharmaceutical drugs such as vaccines, antibodies, and
therapeutic proteins and such crops are called Pharmacrops
 Production of spider silk proteins in tobacco and potato.
 1st human biologic drug derived from the milk of goats that
have been genetically engineered to produce human anti-
thrombin in their milk.
 In Feb 2009, the U.S. Food and Drug Administration (FDA)
approved recombinant anti-thrombin prevention of blood
clots, hereditary anti-thrombin deficiency.
My ppt of gmo
POPULATION GROWTH AND HUNGER INDEX
0 10 20 35 50
GLOBAL HUNGER INDEX SEVERITY SCALE
≤ 9.9 (low) 10.0-19.9
(moderate)
20.0-34.9
(serious)
35.0-49.9
(alarming)
50.0≤
( extremely
alarming)
My ppt of gmo
Indian Perspective
AGRICULTURE IN INDIA
• Agriculture plays a vital role in India’s economy.
• 56.7% of the population is engaged in agriculture (Kumar et
al,2012).
CONSUMPTION OF PESTICIDES
Source: Agarwal A., 2010 Source: Kumar e al., 2012
Its depressing that despite India is producing sufficient food to feed its
population, it is unable to provide access to food to a large no. of people. The
amount of food wasted in our country is staggering.
APPROVED TRANSGENIC HORTICULTURAL CROPS
CROPS TRAITS GENES INTRODUCED
APPLE MODIFIED PRODUCT QUALITY PGAS
BEAN VIRAL DISEASE RESISTANCE Ac1
EGGPLANT LEPIDOPTERAN INSECT RESISTANCE cry1Ac
MELON DELAYED RIPENING/SENESCENCE sam-k
PAPAYA VIRAL DISEASE RESISTANCE prsv_cp, prsv_re
PLUM VIRAL DISEASE RESISTANCE ppv_c
POTATO COLEOPTERAN INSECT RESISTANCE
VIRAL DISEASE RESISTANCE
cry3A
pvy_cp
SQUASH STACKED VIRAL DISEASE RESISTANCE cmv_cp, zymv_cp, wmv_cp
SUGAR BEET GLUFOSINATE HERBICIDE TOLERANCE Pat
SWEET PEPPER VIRAL DISEASE RESISTANCE cmv_cp
TOMATO
SOURCE: ISAAA GMO
DELAYED RIPENING/SENESCENCE
LEPIDOPTERAN INSECT RESISTANCE
DELAYED FRUIT SOFTENING
APPROVED DATABASE
anti-efe, sam k, pg (sense or antisense), acc
(truncated), accd, pg (sense or antisense)
cry1Ac
pg (sense or antisense)
EXAMPLES OF GM-CROPS
• Genetic engineering for Moisture deficit stress tolerance
• Genetic engineering for Salinity tolerance
CROPS GENE TARGET TRAIT REFERENCES
POTATO TPS 1 Drought tolerance Yeo et al (2000)
APPLE Osmyb4 Drought stress Pasquali et al (2008)
TOMATO CBF1 Oxidative stress as well
as water deficit stress
tolerance
Hsieh et al (2002)
BANANA MusaWRKY71 Oxidative and salt stress
tolerance
Shekhawat and
Ganapathi (2013)
CROPS GENE TARGET TRAIT REFERENCES
STRAWBERRY
Osmotin Salt tolerance Husaini (2008)
CARROT Betaine aldehyde dehydrogenase Salt tolerance Kumar et al (2004)
Capsicum annum Osmotin Salt tolerance Subramanayam et al
(2011)
TOMATO SlMBP11 Salt stress tolerance Guo et al (2016)
• Genetic engineering for Cold tolerance
• Genetic engineering for Biotic stress resistance
CROPS GENES TARGET TRAIT REFERENCES
CUCUMBER Dehydrin DHN10 DHN24 Chilling tolerance Yin et al (2004)
GRAPEVINE DREB1b Cold tolerance Jin et al (2007)
APPLE MsDREB6.2 Drought tolerance Liao et al (2016)
TOMATO codA Chilling tolerance Park et al (2004)
CROPS GENES TARGET TRAIT REFERENCES
APPLE Lytic peptide attacin E
Cysteine proteinases
Fire blight resistance
Nematode tolerance
Ko et al (2000)
Roderick et al (2012)
GRAPES Antifreezing protein Low-temperature tolerance Gutoranov et al. (2001)
PINEAPPLE
chitinase (chi), ap24 Heart and root rot resistance Espinosa et al. (2002)
SQUASH Coat protein genes CMV, ZYMV, and WMV Fuchs and Gonsalves
(2007)
Lettuce Coat protein Mirafiori lettuce virus Yoichi et al. (2009)
GLOBAL AREA OF BIOTECH CROPS IN
2017
YEAR HECTARES (million) ACRES(million)
1996 1.7 4.2
2001 52.6 130.0
2003 67.7 167.3
2004 81.0 200.2
2006 102.0 252.0
2010 148.0 365.7
2011 160.0 395.4
2012 170.3 420.8
2013 175.2 432.9
2014 181.5 448.5
2015 179.7 444.0
2016 185.1 457.4
2017* 189.8 469.0
TOTAL 2,339.5 5.780
Global Area of Biotech crops, 22 years, 1996 to 2017. (source: ISAAA, 2017)
*Global area of biotech crops in 2017 increased to 189.8 million hectares compared with 185.1 million hectares in
2016, equivalent to 3% or 4.7 million hectares (11.6 million acres).
BIOTECH CROPS IN INDUSTRIALIZED AND DEVELOPING COUNTRIES
Global Area of Biotech Crops, 2015 and 2017: Industrialized and developing
countries (Million Hectares). (Source: ISAAA, 2017)
TOP TEN BIOTECH CROP COUNTRIES (SOURCE: ISAAA, 2017)
TOP 10 COUNTRIES
Source: Noreo D., 2015
BIOTECH CROP IN INDIA- Bt COTTON
Source: Seetharam G., 2018
GOLDEN RICE
EDIBLE VACCINE
My ppt of gmo
CONs Of GM-CROPS
PROs OF GM-CROPS
Source: ISAAA Briefs, brief 53, Global Status of Commercialized Biotech/GM
Crops in 2017.
Source: Norris M.L., 2015
My ppt of gmo
HOW GMO ARE REGULATED?
Regulation of GM crops in USA and EU (Source: Lau J., 2015)
Regulation of GM crops in India (Source: Dang et al., 2015)
Various organisation perceptions
• Leading voices opposing GMOs
• Greenpeace
• Fairtrade International
Six Corporations
Monsanto
DuPont
Dow
Syngenta
Bayer
BASF
What's wrong with genetic engineering (GE)?
Arguments given by Anti-GMO Voices
• GMOs can spread through nature via cross-pollination from field to field and interbreed
with natural organisms, thereby making it impossible to truly control how GE modified
crops spread
• GMOs cannot be recalled once released into environment
• GMOs should not be released into environment since there is not an adequate scientific
understanding of their impact on environment and human health
• Oppose all patents on plants, animals and humans, as well as patents on their genes
• Biology is not an industrial commodity
• Contamination of conventional crops and wild plants, potential damage to
wildlife, and the uncertain effects on human health when consuming these
foods
• Farmers lose control of seeds they once used and are unable to continue
using ecological pest management
• Instead, farmers have no choice but to buy full package of pesticides that
need to be used with GE seeds to ensure they perform
REFERENCES
• Yeo, E.T., Kwon, H.B., Han, S.E., Lee, J.T., Ryu, J.C., Byu, M.O., 2000. Genetic
engineering o f drought resistant potato plants by introduction of the trehalose-6-
phosphate synthase (TPS1) gene from Saccharomyces cerevisiae. Mol. Cells 10, 263–268.
• Pasquali, G., Biricolti, S., Locatelli, F., et al., 2008. Osmyb4 expression improves adaptive
responses to drought and cold stress in transgenic apples. Plant Cell Rep. 27, 1677.
• Hsieh, T.H., Lee, J.T., Chang, Y.Y., Chan, M.T., 2002a. Tomato plants ectopically
expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress. Plant
Physiol. 130, 618–626.
• Shekhawat, U.K.S., Ganapathi, T.R., 2013. MusaWRKY71 overexpression in banana plants
leads to altered abiotic and biotic stress responses. PLoS One 8 (10), e75506.
• Yin, Z., Pawlowicz, I., Bartoszewski, G., Malinowski, R., Malepszy, S., Rorat, T., 2004.
Transcriptional expression of a Solanum sogarandinum GTDhn10 gene fusion in
cucumber and its correlation with chilling tolerance in transgenic seedlings. Cell. Mol.
Biol. Lett. 9, 891–902.
• Jin, C.W., You, G.Y., He, Y.F., Tang, C., Wu, P., Zheng, S.J., 2007. Iron deficiency-induced
secretion of phenolics facilitates the reutilization of root apoplastic iron in red clover.
Plant Physiol. 144, 278–285.
• Liao, X., Liu, G., Guo, X., Wang, Q., Li, T., Wang, Y., Zhao, D., Yao, L., Wang, S., 2016.
Over-expression of MsDREB6.2 results in cytokinin-deficient developmental phenotypes
and enhances drought tolerance in transgenic apple plants. Plant J. 89.
• Park, E.J., Jekni, Z., Sakamoto, A., DeNoma, J., Yuwansiri, R., Murata, N., Chen, T.H.H.,
2004. Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants,
and flowers from chilling damage. Plant J. 40, 474–487.
• Roderick, H., Tripathi, L., Babirye, A., Wang, D., Tripathi, J., Urwin, P.E., Atkinson, H.J.,
2012. Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic
nematodes. Mol. Plant Pathol. 13, 842-851.
• Ko, K., Norelli, J.L., Reynoird, J.P., Boresjza-Wysocka, E., Brown, S.K., Aldwinckle, H.S.,
2000. Effect of untranslated leader sequence of AMV RNA 4 and signal peptide of
pathogenesis-related protein 1b on attacin gene expression, and resistance to fire blight in
transgenic apple. Biotechnol. Lett. 22, 373–381.
• Gutoranov, G.P., Tsvetkov, I.J., Colova-Tsolova, V.M., Atanassov, A.I., 2001. Genetically
engineered grapevines carrying GFLV coat protein and antifreeze genes. Agric. Conspec.
Sci. 66, 71–76.
• Espinosa, P., Lorenzo, J.C., Iglesias, A., Yabor, L., Menéndez, E., Borroto, J., Hernández,
L., Arencibia, A.D., 2002. Production of pineapple transgenic plants assisted by
temporary immersion bioreactors. Plant Cell Rep. 21, 136–140.
• Fuchs, M., Gonsalves, D., 2007. Safety of virus resistant transgenic plants two decades
after their introductions: lessons from realistic field risk assessment studies. Annu. Rev.
Phytopathol. 45, 173.
• Yoichi, K., Ryoi, F., Yuji, N., 2009. Transgenic resistance to Mirafiori lettuce virus in
lettuce carrying inverted repeats of the viral coat protein gene. Transgenic Res. 18, 113–
120.
• Saraswathi M.S. et al., 2018. Critical evaluation of the benefits and risks of genetically
modified horticultural crops. Elsevier Inc. 316-351.
• Maghari et al., 2011, Genetically Modified Foods and Social Concerns. Reproductive
Biotechnology Research Center. 3, 109-117.
• : www.biotechinstitute.org/download/files/YourWorld/yw-101-food
• Buiatti M. et al., 2012. The application of GMOs in agriculture and in food production for
a better nutrition: two different scientific points of view. Genes Nutr. 8, 255-270.
• U.S. census beaureau, International database, August 2016 updates.
• Grebmer K.V. et al., 2017. Global Hunger Index: The inequality of Hunger. International
food policy research Institue.Washington. 5-46.
• Agarwal A., 2010. Water Pollution with Special Reference to Pesticide Contamination in
India. Journal of Water Resource and Protection. 05, 432-448.
• Kumar e al., 2012. Harmful effects of pesticide on Human health. Annal of Agri Bio
research, 17, 125-127.
• Seetharam G., 2018, These two issues could put the brakes on Bt cotton story. The
Economic Times.
• ISAAA Briefs, brief 53, Global Status of Commercialized Biotech/GM Crops in 2017.
• Noreo D., 2015, Latin American GMO powerhouse Brazil expands portfolio of crops with
intro of new herbicide resistant soy. Genetic Literacy project.
• Norris M.L., 2015, Will GMOs Hurt My Body? The Public’s Concerns and How Scientists
Have Addressed Them. Science in the news.
• Lau J., 2015, Same Science, Different Policies: Regulating Genetically Modified Foods in the
U.S. and Europe. Science in the news.
• Dang, H., Gilmour, B., & Kishor, N. (2015). India’s agri-biotech policies, regulations, and
decision-making. AgBioForum, 18, 87-97

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My ppt of gmo

  • 1. SEMINAR REPORT ON GENETICALLY MODIFIED CROP SUBMITTED TO I.K.GUJRAL PUNJAB TECHNICAL UNIVERSITY KAPURTHALA, PUNJAB BY (2017-2019) SAKSHI THAKUR REG NO. 1732065 DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY
  • 3. What Are Genetically Modified Foods •Any food containing or derived from a genetically engineering organisms (plants, animals or microorganisms).
  • 4. Background • As we entered the 21st century, industrialized countries struggling with side effects of, ‘GREEN-REVOLUTION’. • Worse, many of the world’s poor have never benefitted from Green Revolution because it did not solve underlying problem, ‘POVERTY’ • Now, WHAT CAN HELP THEM?  Many scientist think a new ‘GENE REVOLUTION’, uses biotechnology to create a new ‘Genetically Modified’ or ‘GM CROPS’. • But, some people worry that whether, ‘These crops are safe or not’ Source: www.biotechinstitute.org/download/files/YourWorld/yw-101-food
  • 5. GM FOOD FROM GM CROPS
  • 6. HOW GENETICALLY MODIFIED ORGANISMS PRODUCED? • Genetically Modified Organisms (GMOs) are being made by inserting a gene from an external source such as viruses, bacteria, animals or plants into usually unrelated species.
  • 7. Contd…. Types of Crop FRUITS VEGETABLES ORNAMENTALS PLANTATION CROPS • Genetically modified crops or Transgenic crops carrying the novel traits and released for commercial agriculture production. • These include: Pest resistant cotton, maize, canola (mainly Bt or Bacillus thuringiensis), herbicide glyphosate resistant soybean, cotton and viral disease resistant potatoes, papaya and squash. GENETICALLY MODIFIED CROPS VIRUS TOLERANCE DROUGHT RESISTANCE
  • 8. GENERATION OF GMOs FIRST GENERATION SECOND GENERATION THIRD GENERATION  Enhance crop productivity  Traits such as herbicide tolerant, better insect tolerant, and better tolerance to environmental stress.  They are not significantly different from traditional crops in terms of appearance, taste and nutrition.  Examples: Corn, soybean etc.  Concerns product quality characteristics such as having better taste, more nutritional content, and having longer shelf life.  Example: Flavr savr tomato, Potato.  Use of plants to make cheaper, more efficiently produced and more plentiful industrial products, such as biosensors, industrial enzymes and epoxies, plastics and cosmetics, and pharmaceutical drugs such as vaccines, antibodies, and therapeutic proteins and such crops are called Pharmacrops  Production of spider silk proteins in tobacco and potato.  1st human biologic drug derived from the milk of goats that have been genetically engineered to produce human anti- thrombin in their milk.  In Feb 2009, the U.S. Food and Drug Administration (FDA) approved recombinant anti-thrombin prevention of blood clots, hereditary anti-thrombin deficiency.
  • 10. POPULATION GROWTH AND HUNGER INDEX
  • 11. 0 10 20 35 50 GLOBAL HUNGER INDEX SEVERITY SCALE ≤ 9.9 (low) 10.0-19.9 (moderate) 20.0-34.9 (serious) 35.0-49.9 (alarming) 50.0≤ ( extremely alarming)
  • 14. AGRICULTURE IN INDIA • Agriculture plays a vital role in India’s economy. • 56.7% of the population is engaged in agriculture (Kumar et al,2012).
  • 16. Source: Agarwal A., 2010 Source: Kumar e al., 2012
  • 17. Its depressing that despite India is producing sufficient food to feed its population, it is unable to provide access to food to a large no. of people. The amount of food wasted in our country is staggering.
  • 18. APPROVED TRANSGENIC HORTICULTURAL CROPS CROPS TRAITS GENES INTRODUCED APPLE MODIFIED PRODUCT QUALITY PGAS BEAN VIRAL DISEASE RESISTANCE Ac1 EGGPLANT LEPIDOPTERAN INSECT RESISTANCE cry1Ac MELON DELAYED RIPENING/SENESCENCE sam-k PAPAYA VIRAL DISEASE RESISTANCE prsv_cp, prsv_re PLUM VIRAL DISEASE RESISTANCE ppv_c POTATO COLEOPTERAN INSECT RESISTANCE VIRAL DISEASE RESISTANCE cry3A pvy_cp SQUASH STACKED VIRAL DISEASE RESISTANCE cmv_cp, zymv_cp, wmv_cp SUGAR BEET GLUFOSINATE HERBICIDE TOLERANCE Pat SWEET PEPPER VIRAL DISEASE RESISTANCE cmv_cp TOMATO SOURCE: ISAAA GMO DELAYED RIPENING/SENESCENCE LEPIDOPTERAN INSECT RESISTANCE DELAYED FRUIT SOFTENING APPROVED DATABASE anti-efe, sam k, pg (sense or antisense), acc (truncated), accd, pg (sense or antisense) cry1Ac pg (sense or antisense)
  • 19. EXAMPLES OF GM-CROPS • Genetic engineering for Moisture deficit stress tolerance • Genetic engineering for Salinity tolerance CROPS GENE TARGET TRAIT REFERENCES POTATO TPS 1 Drought tolerance Yeo et al (2000) APPLE Osmyb4 Drought stress Pasquali et al (2008) TOMATO CBF1 Oxidative stress as well as water deficit stress tolerance Hsieh et al (2002) BANANA MusaWRKY71 Oxidative and salt stress tolerance Shekhawat and Ganapathi (2013) CROPS GENE TARGET TRAIT REFERENCES STRAWBERRY Osmotin Salt tolerance Husaini (2008) CARROT Betaine aldehyde dehydrogenase Salt tolerance Kumar et al (2004) Capsicum annum Osmotin Salt tolerance Subramanayam et al (2011) TOMATO SlMBP11 Salt stress tolerance Guo et al (2016)
  • 20. • Genetic engineering for Cold tolerance • Genetic engineering for Biotic stress resistance CROPS GENES TARGET TRAIT REFERENCES CUCUMBER Dehydrin DHN10 DHN24 Chilling tolerance Yin et al (2004) GRAPEVINE DREB1b Cold tolerance Jin et al (2007) APPLE MsDREB6.2 Drought tolerance Liao et al (2016) TOMATO codA Chilling tolerance Park et al (2004) CROPS GENES TARGET TRAIT REFERENCES APPLE Lytic peptide attacin E Cysteine proteinases Fire blight resistance Nematode tolerance Ko et al (2000) Roderick et al (2012) GRAPES Antifreezing protein Low-temperature tolerance Gutoranov et al. (2001) PINEAPPLE chitinase (chi), ap24 Heart and root rot resistance Espinosa et al. (2002) SQUASH Coat protein genes CMV, ZYMV, and WMV Fuchs and Gonsalves (2007) Lettuce Coat protein Mirafiori lettuce virus Yoichi et al. (2009)
  • 21. GLOBAL AREA OF BIOTECH CROPS IN 2017
  • 22. YEAR HECTARES (million) ACRES(million) 1996 1.7 4.2 2001 52.6 130.0 2003 67.7 167.3 2004 81.0 200.2 2006 102.0 252.0 2010 148.0 365.7 2011 160.0 395.4 2012 170.3 420.8 2013 175.2 432.9 2014 181.5 448.5 2015 179.7 444.0 2016 185.1 457.4 2017* 189.8 469.0 TOTAL 2,339.5 5.780 Global Area of Biotech crops, 22 years, 1996 to 2017. (source: ISAAA, 2017) *Global area of biotech crops in 2017 increased to 189.8 million hectares compared with 185.1 million hectares in 2016, equivalent to 3% or 4.7 million hectares (11.6 million acres).
  • 23. BIOTECH CROPS IN INDUSTRIALIZED AND DEVELOPING COUNTRIES Global Area of Biotech Crops, 2015 and 2017: Industrialized and developing countries (Million Hectares). (Source: ISAAA, 2017)
  • 24. TOP TEN BIOTECH CROP COUNTRIES (SOURCE: ISAAA, 2017) TOP 10 COUNTRIES
  • 26. BIOTECH CROP IN INDIA- Bt COTTON Source: Seetharam G., 2018
  • 32. Source: ISAAA Briefs, brief 53, Global Status of Commercialized Biotech/GM Crops in 2017. Source: Norris M.L., 2015
  • 34. HOW GMO ARE REGULATED? Regulation of GM crops in USA and EU (Source: Lau J., 2015)
  • 35. Regulation of GM crops in India (Source: Dang et al., 2015)
  • 36. Various organisation perceptions • Leading voices opposing GMOs • Greenpeace • Fairtrade International Six Corporations Monsanto DuPont Dow Syngenta Bayer BASF
  • 37. What's wrong with genetic engineering (GE)? Arguments given by Anti-GMO Voices • GMOs can spread through nature via cross-pollination from field to field and interbreed with natural organisms, thereby making it impossible to truly control how GE modified crops spread • GMOs cannot be recalled once released into environment • GMOs should not be released into environment since there is not an adequate scientific understanding of their impact on environment and human health • Oppose all patents on plants, animals and humans, as well as patents on their genes • Biology is not an industrial commodity
  • 38. • Contamination of conventional crops and wild plants, potential damage to wildlife, and the uncertain effects on human health when consuming these foods • Farmers lose control of seeds they once used and are unable to continue using ecological pest management • Instead, farmers have no choice but to buy full package of pesticides that need to be used with GE seeds to ensure they perform
  • 39. REFERENCES • Yeo, E.T., Kwon, H.B., Han, S.E., Lee, J.T., Ryu, J.C., Byu, M.O., 2000. Genetic engineering o f drought resistant potato plants by introduction of the trehalose-6- phosphate synthase (TPS1) gene from Saccharomyces cerevisiae. Mol. Cells 10, 263–268. • Pasquali, G., Biricolti, S., Locatelli, F., et al., 2008. Osmyb4 expression improves adaptive responses to drought and cold stress in transgenic apples. Plant Cell Rep. 27, 1677. • Hsieh, T.H., Lee, J.T., Chang, Y.Y., Chan, M.T., 2002a. Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress. Plant Physiol. 130, 618–626. • Shekhawat, U.K.S., Ganapathi, T.R., 2013. MusaWRKY71 overexpression in banana plants leads to altered abiotic and biotic stress responses. PLoS One 8 (10), e75506. • Yin, Z., Pawlowicz, I., Bartoszewski, G., Malinowski, R., Malepszy, S., Rorat, T., 2004. Transcriptional expression of a Solanum sogarandinum GTDhn10 gene fusion in cucumber and its correlation with chilling tolerance in transgenic seedlings. Cell. Mol. Biol. Lett. 9, 891–902. • Jin, C.W., You, G.Y., He, Y.F., Tang, C., Wu, P., Zheng, S.J., 2007. Iron deficiency-induced secretion of phenolics facilitates the reutilization of root apoplastic iron in red clover. Plant Physiol. 144, 278–285.
  • 40. • Liao, X., Liu, G., Guo, X., Wang, Q., Li, T., Wang, Y., Zhao, D., Yao, L., Wang, S., 2016. Over-expression of MsDREB6.2 results in cytokinin-deficient developmental phenotypes and enhances drought tolerance in transgenic apple plants. Plant J. 89. • Park, E.J., Jekni, Z., Sakamoto, A., DeNoma, J., Yuwansiri, R., Murata, N., Chen, T.H.H., 2004. Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage. Plant J. 40, 474–487. • Roderick, H., Tripathi, L., Babirye, A., Wang, D., Tripathi, J., Urwin, P.E., Atkinson, H.J., 2012. Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes. Mol. Plant Pathol. 13, 842-851. • Ko, K., Norelli, J.L., Reynoird, J.P., Boresjza-Wysocka, E., Brown, S.K., Aldwinckle, H.S., 2000. Effect of untranslated leader sequence of AMV RNA 4 and signal peptide of pathogenesis-related protein 1b on attacin gene expression, and resistance to fire blight in transgenic apple. Biotechnol. Lett. 22, 373–381. • Gutoranov, G.P., Tsvetkov, I.J., Colova-Tsolova, V.M., Atanassov, A.I., 2001. Genetically engineered grapevines carrying GFLV coat protein and antifreeze genes. Agric. Conspec. Sci. 66, 71–76. • Espinosa, P., Lorenzo, J.C., Iglesias, A., Yabor, L., Menéndez, E., Borroto, J., Hernández, L., Arencibia, A.D., 2002. Production of pineapple transgenic plants assisted by temporary immersion bioreactors. Plant Cell Rep. 21, 136–140.
  • 41. • Fuchs, M., Gonsalves, D., 2007. Safety of virus resistant transgenic plants two decades after their introductions: lessons from realistic field risk assessment studies. Annu. Rev. Phytopathol. 45, 173. • Yoichi, K., Ryoi, F., Yuji, N., 2009. Transgenic resistance to Mirafiori lettuce virus in lettuce carrying inverted repeats of the viral coat protein gene. Transgenic Res. 18, 113– 120. • Saraswathi M.S. et al., 2018. Critical evaluation of the benefits and risks of genetically modified horticultural crops. Elsevier Inc. 316-351. • Maghari et al., 2011, Genetically Modified Foods and Social Concerns. Reproductive Biotechnology Research Center. 3, 109-117. • : www.biotechinstitute.org/download/files/YourWorld/yw-101-food • Buiatti M. et al., 2012. The application of GMOs in agriculture and in food production for a better nutrition: two different scientific points of view. Genes Nutr. 8, 255-270. • U.S. census beaureau, International database, August 2016 updates. • Grebmer K.V. et al., 2017. Global Hunger Index: The inequality of Hunger. International food policy research Institue.Washington. 5-46. • Agarwal A., 2010. Water Pollution with Special Reference to Pesticide Contamination in India. Journal of Water Resource and Protection. 05, 432-448. • Kumar e al., 2012. Harmful effects of pesticide on Human health. Annal of Agri Bio research, 17, 125-127.
  • 42. • Seetharam G., 2018, These two issues could put the brakes on Bt cotton story. The Economic Times. • ISAAA Briefs, brief 53, Global Status of Commercialized Biotech/GM Crops in 2017. • Noreo D., 2015, Latin American GMO powerhouse Brazil expands portfolio of crops with intro of new herbicide resistant soy. Genetic Literacy project. • Norris M.L., 2015, Will GMOs Hurt My Body? The Public’s Concerns and How Scientists Have Addressed Them. Science in the news. • Lau J., 2015, Same Science, Different Policies: Regulating Genetically Modified Foods in the U.S. and Europe. Science in the news. • Dang, H., Gilmour, B., & Kishor, N. (2015). India’s agri-biotech policies, regulations, and decision-making. AgBioForum, 18, 87-97

Editor's Notes

  • #3: Goodorning one n all present here myself sakshi Thakur and my topic is about genetic modified food.
  • #4: So lets start with the basic definition of GM food. Genetically engineered organisms could be plant animals or mo.
  • #5: Lets start with the Basic Introduction.. Green revolution was initiated in 1960s in order to solve the issue of malnutrition in developing countries. Now what the green revolution is? GR technology involves the use of bioengineered seed that work in conjunction with chemical fertilizers and heavy irrigation so that we get increased yield of crop. The technology was readily adopted in India and was a great success. However there are farmers who could not afford inputs necessary to participate in green revolution like lack funds to take advantage of technology, insufficient info and resources available to small farmers to apply technology and absence of gov. support for small farmers, and gap between social classes widened as wealthy farmers got wealthier and poor farmers lagged behind. So inorder to solve these issues scientists thought a new gene revolution that uses biotechnology to create a new GM crops. But some peope worry that whetehre these crops are safe or not.
  • #6: As you can see in this table…There are gm crops and gm food derived from these crops.
  • #7: Now lets see how gmos are produced….GMO could be plant animal and mo. And natural DNA of paticular organisms has been altered via different techniques. This technology is called GE or Modern biotechnology. Now here I this picture u can see that there is a bacterial cell having a gene that produces pest killing protein which is taken out via biochemical scissors and pasted into plants DNA using molecular techniques. Now normal plant clell turned into GM cell which can produce pest resistant protein and then a whole plant can be produced from single GM cell n finally in result we got a pest resistant plant.
  • #9: Now the generation of gmos..there are three generation of gmos…The first gen is only specified to enhance crop productivity like herbicide toleratnt insect tolerant etc. In 2nd gen. product quality characters such as better taste better nutritional content longer shelf life is enhanced and in third gen plants are used for production of industrial products like biosensore plastiics cosmetics and drugs like vaccines antioboied and the crops used for such production is called Pharmacrops. Eg maize engineered to express human gastric lipase used to treat cystic fibrosis.
  • #10: Now it was all about the basic introduction of GMOS…Now come to the fact that why we need Gm crops.
  • #11: As we know the whole wolrd is facing a several challenges like population growth problem of malnutrition, climatechange , co2 conc. Isbincreasing crop loss due to pest infestation and food is not avalaible to all. So GM may be the solution for these problems.. As shown here in the graph current population is 7.3 billion which is expected to reach 8.5 billion by 2030 and 9.7 billion by 2050.
  • #12: Now here in this slide global hunger index is shown…GHI track and measure hunger globally and by country and by region. The problems of hunger is measured in 5 categories….zero is the best score and 100 is worst
  • #13: Now if we talk about the indian states then Punjab is the best best performing state in term of hunger and the worst performing states are Mp Jharkhand bihar and chatisgarh
  • #15: It is estimated that around 56.7% peopleare engaged in agriculture so agriculture is backbone of India economy. But Indian agriculture is facing many problems like crop loses due to pests as you can see crop loss due to ….And if we see loss in terms of rupees it is 60,000 crore. In other picture u can see 2 types of losses post harvest and pre harvest…So overall loss from pests are as hgh as 5%.So farmers are using pesticide to protect their food products their crops in form of herbi, weedi, fungicide etc.
  • #16: Here in the pie chart we can see In india there is 75% consumption of insecticide…but in world there less consumption of insecticide n more consumption of herbicide.The next pie chart showing statewise consumption of pesticide in india ….Ap telangana, Punjab, Maharashtra lead in consumption of pesticide
  • #17: This is showing pesticide cycle in nature..As the farmer sprayin pesticide on the plants some amount of pesticides get absorbed into soil ……and again it may come to earth surface by raining…and in result it can affect human via food chain..Now the harmful effects of pesticieds..Pesticide can get into the body by oral exposure, inhalation exposure, skin…Toxic effects depends on exposure time and concentration of pesticide. And exposure colud be chronic and acute… and can resukts into various health effects like
  • #19: This is the list of approved transgenic horticulture crops by ISAAA…ie International service for acquisition of AGRI biotech application is a non profit organisation focusing on genetic engineering.
  • #20: These are the examples of some gm crops with different target trait and different genes.
  • #24: The 3.7 million hectares increment in the industrialized countries between 2016 and 2017 is due mainly to increases in the USA at 3% and Canada at 13%, as well as small increases in Australia, Spain and Portugal. Increases in developing countries, led by India at 6%, Brazil and Pakistan at 3%, and increases in the biotech area in Bolivia, Vietnam, Chile and most especially in Bangladesh at 242%
  • #25: These are the top 10 biotech crop counries in which usa is leading which grew 75 million hectares followed by brazil argentina and Canada…India is in the 5th position
  • #26: These are crop mainly grown in these countries..In usa maize soybean cotton etc are the major gm crops and in India mainly the bt cotton is grown.
  • #27: So this is the major examples of biotech
  • #28: Golden rice is gm rice which is modified to enhance its nutritional value .it c
  • #35: Now lets see how the gmos are regulated in different countries…This picture is showing the regulation of gmo in European union and US.. US approach is to focus on the product n the U.S., the same regulations apply to GM and conventional foods because despite the different processes used to make them, the final products are considered to be similar. The document concluded that foods made with genetic engineering techniques are not fundamentally different from conventional foods in terms of overall composition, so there was no need for legislation specifically dealing with GM foods. In other words, agencies should use the same regulatory processes to evaluate conventional and GM foods. he document established that regulation should focus on the nature of the final food product rather than the process by which the food product is made. 3 federal agencies playing role FDA, USDA, EPA. The Food and Drug Administration (FDA) evaluates the safety of substances added to foods, such as color dyes, artificial sweeteners, and genetically engineered proteins. The U.S. Department of Agriculture (USDA) prevents the spread of potentially invasive new plants within the U.S., including plants imported from other countries and GM crops. The Environmental Protection Agency (EPA) determines the risks of pesticides and chemicals to human and environmental health, whether the substances are applied traditionally as a spray or expressed by the GM crop [5]. Because GM foods fall under the FDA classification of  “generally recognized as safe,” they typically do not require special labeling or premarket approval, that is, they do not have to be approved before entering the market.   The FDA recommends that companies go through a voluntary consultation process to determine whether their new GM food would require premarket approval. Approval is necessary if the GM food contains high levels of toxic substances or allergens or reduced levels of important nutrients, but this is rarely the case.  Companies can gain this exemption by demonstrating that the GM food is “substantially equivalent” to a prior existing food in terms of composition, nutrition, and safety. he USDA requires companies to submit a wide range of data before GM plants can be introduced in U.S. fields under regulated or nonregulated status. The EPA’s regulations on how pesticides affect human and environmental health extend to GM plants that produce insecticidal substances . The EU focus on the process . Compared to the U.S., the EU imposes strict regulations on GM food. In the EU, all GM food products must go through a centralized process for premarket approval and follow labeling guidelines,. . When companies submit applications for GM food approval to an EU member state, the centralized European Food Safety Authority (EFSA) conducts scientific risk assessments. The EFSA is an independent scientific committee that evaluates the human and environmental health risks posed by GM foods, using the same types of data required by the various U.S. regulatory agencies.  Following approval, GM foods on the market must adhere to labeling guidelines. Specifically, food products containing more than 0.9% GM material must be labeled as being GM foods [4].
  • #36: Now see the biotech regulatory in india…Three central governmental ministries—MOST (DBT), MOEF, and MOA—are involved in this governance, along with a number of risk-management institutions within or outside of these ministries  either the central or national level. GMOs and relevant products in India are regulated as per the Rules 1989 implemented by MOEF. These rules are enforced by both MOEF and DBT through the authorized institutions identified under the Rules, which mainly include rDNA Advisory Committee (RDAC), Institutional Biosafety Committee (IBSC), Review Committee on Genetic Manipulation (RCGM), Genetic Engineering Appraisal Committee (GEAC), State Biotech Coordination Committee (SBCC), and District Level Coordination Committee (DLCC; MOST, DBT, 2011). While the RDAC is advisory only, IBSC, RCGM, and GEAC are involved in regulatory enforcemen. RCGM brings manual and guidline forspecifying project and geac gives approval for usage of hazardus mo and gmos relase to env and experimental trial.