nano-bubble
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1) Nano-bubbles are smaller than microbubbles and have a longer lifetime, lasting several months compared to microbubbles which shrink and disappear within minutes. 2) Nano-bubbles are stabilized by a mechanism where ions accumulate on their surface, preventing the gas from dispersing and allowing the bubbles to persist for longer. 3) Ozone nano-bubbles are more effective at disinfecting and sterilizing than conventional ozone water methods, able to inactivate over 99% of viruses within 1 day.
nano-bubble
- 1. Fantastic Properties of Nano-bubbles Kaneo Chiba Masayoshi Takahashi
- 3. Microbubble Collapse Physical stimulus Nano-bubble production by collapsing microbubble Radical generation ・OH Decomposition of organic chemicals Waste water treatment Physical stimulus Nanobubble production Collapse Microbubble with salt (electrolytes) Nano-bubble ・OH ４
- 5. Analysis of nano-bubble by Dynamic light scattering (DLS) 0 2 4 6 8 10 12 14 16 18 20 76 93 113 138 168 204 249 304 370 Particle size (nm) ６
- 6. Microbubble 10 ～30μm Nano-bubble < 200nm The deference of size between MB and NB ７
- 7. Shrinking Disappear Several minutes Several months Microbubble Nanobubble Continue The deference of life time between MB and NB Why ? ８
- 8. Movement of microbubbles in electrophoresis cell Light Microscope Electrode Electrophoresis cell Water + microbubble Electrode ( + ↔ − ) (− ↔ +) ９
- 9. time ζpotential（mV） Change in ζ potential of microbubble over time Diameter （μm） 0 5 10 15 20 25 30 -20 -30 -40 -50 -60 -70 -80 Accumulation of surrounding ions Ions Microbubble time Ions １０ with salt (electrolytes)
- 10. Aqueous solution Nano-bubble ＋ ＋ ＋ ＋ ＋ ＋ ＋ － － － － － － － － ＋ ＋ ＋ ＋ ＋ － ＋ － ＋ ＋ ＋ ＋ ＋ ＋ ＋ Stabilization mechanism of nanobubble (assumption) Negatively charged Surrounded by accumulated ions (counter ions) Prevention of gas dispersion Salting out １１
- 12. Normal ozone water vs. Ozone nano-bubble Ozone water ; disinfection & sterilizing （Food industry, medical ） 1 hour 100 days Bubbling Micro-bubble Nano-bubble 0.1mg/L 1.5mg/L 1.5mg/L More than 1.0mg/L Ozone Normal method Nano-bubble Collapse 13
- 13. Oyster Norovirus Norovirus symptoms: vomiting, diarrhea, a low-grade fever, chills, headache, muscle aches During cultivation How to treat the problem of norovirusHow to treat the problem of norovirus 14
- 14. Chlorine ( high concentration ) ----- not good for foods Chlorine Norovirus Conventional method Oyster Ozone water Oyster Ozone = strong oxidation ability ↓ Virus → inactivation Norovirus 15
- 15. Oysters in ozone nanobuble water for 1 day 16
- 16. Before ozone nanobubble treatment 17
- 17. More than 99% of feline calicivirus in the body --- inactivated After ozone nanobubble treatment 18
- 18. Practical method for inactivation of norovirus by ozone nano-bubble 19
- 19. Carps : recovering from Carp herpes ( serious disease ) Active effect for fishes 20
- 20. Oxidation ability of ozone nano-bubble as a form of mist 21
- 21. more than 80% of original oxidation ability reduction of smell and bacteria from atmospheres 22
- 22. Oxygen nanobubble Collapse of oxygen microbubble About 1% salt Recovery of weakened fishes Active effect for creatures 23
- 24. Orchid in oxygen NB water for more than 1 month Astonishing effects on plant Still alive 25
- 25. Tap water Oxygen nano-bubble water White radish Broccoli Broccoli White radish Initial growth of plants 26
- 26. Active effect for oysters -20℃ for 1 day in oxygen NB Room temperature After 1 hour Still alive 27
- 27. control TNF alpha With oxygen nanobubble control TNF alpha Prevention of arteriosclerosis (hardening of blood vessel ) Oxygen nanobubble inhibits mRNA expression induced by TNFα in RAOECs ICAM1 VCAM1 GAPDH Department of Cardiology, Jichi Medical University Application of nanobubble in medicine Culture aorta endothelium of rat Cytokine stimulation（TNF alpha） Heart attack Stroke 28
- 28. Vagus nerve of rat : after 1 week Isotonic NaCl solution Oxygen nanobubble Tokyo Medical and Dental University Application of nanobubble in medicine Preservation of living tissue / organ 29
- 29. 30 Thank you very much