Protozoa are single-celled, microscopic eukaryotic organisms that can move independently. They are classified into four main phyla: Protozoa, Ciliophora, Sarcomastigophora, and Apicomplexa. Many protozoa are heterotrophic and either free-living or parasitic. They reproduce asexually through binary fission or sexually through conjugation. Some important human diseases caused by protozoan parasites include malaria, amebic dysentery, giardiasis, African sleeping sickness, Chagas disease, and toxoplasmosis.