PhyScreen IMAC Resin Screening Kit for Process Development
- 1. PhyNexus www.phynexus.com 408.267.7214 PhyScreen Ni-IMAC Resin Screening Toolkit Protein purification performance can vary depending on the protein analyzed and the affinity resin utilized. Researchers often need to settle for the best results obtained from a few available purification options. PhyNexus has developed the unique PhyTip® column technology for the small scale affinity purification of engineered proteins and antibodies, which is a powerful and efficient method for generating pure, functional protein. One of the benefits of a high throughput small-scale purification method is the ability to easily and quickly screen many purification options simultaneously. PhyNexus has made this even easier with the availability of the PhyScreen Ni-IMAC screen kit which includes columns of twelve different Ni-IMAC resins, prepared for ease of use with the MEA automated system, or other commercially available liquid handlers. Resin screening is especially valuable when affinity purifying His-tagged recombinant proteins due to the high propensity of background interactions between Ni-IMAC and naturally occurring multiple His residues. • Need a fast answer? Purify small volumes quickly and run 12 resins in parallel. • Scaling up? Optimize easily for the best resin. • Variable buffer conditions? Screen to optimize capture, wash and elution buffer conditions while screening for the best resin. • Packing resins manually? Automatable pre-made kit. • Require finishing columns? Eliminate the sample and time loss associated with running extra gel filtration and ion exchange following purification. Resin: A B C D E F G H The same E. coli lysate sample * expressing 6His-tagged protein was processed by 200µL PhyTip columns containing 5µL of 8 different IMAC resins to capture, purify and enrich the His- tagged protein. The resulting elutions were analyzed by SDS gel and stained with Coomassie. Conclusion: Of the 8 resins screened, Resin E* demonstrated superior purification of the His-tagged protein. For further support call PhyNexus at 408.267.7214 or e-mail support@phynexus.com. Copyright © 2008, PhyNexus, Inc., All Rights Reserved For further information on PhyNexus products visit our website at www.phynexus.com
- 2. PhyNexus www.phynexus.com 408.267.7214 PhyScreen Ni-IMAC Resin Screening Toolkit PhyTip columns are specifically manufactured for PhyTip columns can also be used with the ME 200 the automated MEA bench top, walk-away system. and ME 1000 semi-automated purification systems. The PhyScreen kit includes 200µL columns with 5µL of the following IMAC resins: • HIS-Select® HF Ni Affinity Gel • PrepEase® High Specificity • Ni SepharoseTM 6 FF • PrepEase® High Yield • His-Bind®, Ni Charged • His-CatchTM Metal Chelating Cellulose • Ni Chelating Superflow • Ni-NTA His-Bind® Resin • Procatch His resin • Ni-NTA His-Bind® Superflow • Ni-IDA Resin • HisPur Cobalt Resin Resins not included in this list may also be substituted as a custom order. PhyScreen Part Numbers: Automated System compatibility: PTR 92-05-NS ME or MEA systems, standard Rainin LTS tips PTB 92-05-NS Beckman FX Series PTT 92-05-NS Tecan Temo PTP 92-05-NS Packard P235 tips PTZ 92-05-NS Caliper Sciclone For further support call PhyNexus at 408.267.7214 or e-mail support@phynexus.com. Copyright © 2008, PhyNexus, Inc., All Rights Reserved For further information on PhyNexus products visit our website at www.phynexus.com PhyNexus and PhyTip are registered trademarks of PhyNexus Inc. All other trademarks are the trademarks of their respective owners.